RNA oxidation in chromatin modification and DNA-damage response following exposure to formaldehyde

Abstract Formaldehyde is an environmental and occupational chemical carcinogen implicated in the damage of proteins and nucleic acids. However, whether formaldehyde provokes modifications of RNAs such as 8-oxo-7,8-dihydroguanine (8-oxoG) and the role that these modifications play on conferring long-...

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Autores principales:	Juan C. Gonzalez-Rivera, Mark W. Sherman, Dongyu S. Wang, Jamie C. L. Chuvalo-Abraham, Lea Hildebrandt Ruiz, Lydia M. Contreras
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a6b0a7d779c54fffba24d73f343cad82
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spelling	oai:doaj.org-article:a6b0a7d779c54fffba24d73f343cad822021-12-02T18:37:06ZRNA oxidation in chromatin modification and DNA-damage response following exposure to formaldehyde10.1038/s41598-020-73376-72045-2322https://doaj.org/article/a6b0a7d779c54fffba24d73f343cad822020-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-73376-7https://doaj.org/toc/2045-2322Abstract Formaldehyde is an environmental and occupational chemical carcinogen implicated in the damage of proteins and nucleic acids. However, whether formaldehyde provokes modifications of RNAs such as 8-oxo-7,8-dihydroguanine (8-oxoG) and the role that these modifications play on conferring long-term adverse health effects remains unexplored. Here, we profile 8-oxoG modifications using RNA-immunoprecipitation and RNA sequencing (8-oxoG RIP-seq) to identify 343 RNA transcripts heavily enriched in oxidations in human bronchial epithelial BEAS-2B cell cultures exposed to 1 ppm formaldehyde for 2 h. RNA oxidation altered expression of many transcripts involved in chromatin modification and p53-mediated DNA-damage responses, two pathways that play key roles in sustaining genome integrity and typically deregulated in tumorigenesis. Given that these observations were identified in normal cells exhibiting minimal cell stress and death phenotypes (for example, lack of nuclear shrinkage, F-actin alterations or increased LDH activity); we hypothesize that oxidative modification of specific RNA transcripts following formaldehyde exposure denotes an early process occurring in carcinogenesis analogous to the oxidative events surfacing at early stages of neurodegenerative diseases. As such, we provide initial investigations of RNA oxidation as a potentially novel mechanism underlying formaldehyde-induced tumorigenesis.Juan C. Gonzalez-RiveraMark W. ShermanDongyu S. WangJamie C. L. Chuvalo-AbrahamLea Hildebrandt RuizLydia M. ContrerasNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-16 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Juan C. Gonzalez-Rivera Mark W. Sherman Dongyu S. Wang Jamie C. L. Chuvalo-Abraham Lea Hildebrandt Ruiz Lydia M. Contreras RNA oxidation in chromatin modification and DNA-damage response following exposure to formaldehyde
description	Abstract Formaldehyde is an environmental and occupational chemical carcinogen implicated in the damage of proteins and nucleic acids. However, whether formaldehyde provokes modifications of RNAs such as 8-oxo-7,8-dihydroguanine (8-oxoG) and the role that these modifications play on conferring long-term adverse health effects remains unexplored. Here, we profile 8-oxoG modifications using RNA-immunoprecipitation and RNA sequencing (8-oxoG RIP-seq) to identify 343 RNA transcripts heavily enriched in oxidations in human bronchial epithelial BEAS-2B cell cultures exposed to 1 ppm formaldehyde for 2 h. RNA oxidation altered expression of many transcripts involved in chromatin modification and p53-mediated DNA-damage responses, two pathways that play key roles in sustaining genome integrity and typically deregulated in tumorigenesis. Given that these observations were identified in normal cells exhibiting minimal cell stress and death phenotypes (for example, lack of nuclear shrinkage, F-actin alterations or increased LDH activity); we hypothesize that oxidative modification of specific RNA transcripts following formaldehyde exposure denotes an early process occurring in carcinogenesis analogous to the oxidative events surfacing at early stages of neurodegenerative diseases. As such, we provide initial investigations of RNA oxidation as a potentially novel mechanism underlying formaldehyde-induced tumorigenesis.
format	article
author	Juan C. Gonzalez-Rivera Mark W. Sherman Dongyu S. Wang Jamie C. L. Chuvalo-Abraham Lea Hildebrandt Ruiz Lydia M. Contreras
author_facet	Juan C. Gonzalez-Rivera Mark W. Sherman Dongyu S. Wang Jamie C. L. Chuvalo-Abraham Lea Hildebrandt Ruiz Lydia M. Contreras
author_sort	Juan C. Gonzalez-Rivera
title	RNA oxidation in chromatin modification and DNA-damage response following exposure to formaldehyde
title_short	RNA oxidation in chromatin modification and DNA-damage response following exposure to formaldehyde
title_full	RNA oxidation in chromatin modification and DNA-damage response following exposure to formaldehyde
title_fullStr	RNA oxidation in chromatin modification and DNA-damage response following exposure to formaldehyde
title_full_unstemmed	RNA oxidation in chromatin modification and DNA-damage response following exposure to formaldehyde
title_sort	rna oxidation in chromatin modification and dna-damage response following exposure to formaldehyde
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/a6b0a7d779c54fffba24d73f343cad82
work_keys_str_mv	AT juancgonzalezrivera rnaoxidationinchromatinmodificationanddnadamageresponsefollowingexposuretoformaldehyde AT markwsherman rnaoxidationinchromatinmodificationanddnadamageresponsefollowingexposuretoformaldehyde AT dongyuswang rnaoxidationinchromatinmodificationanddnadamageresponsefollowingexposuretoformaldehyde AT jamieclchuvaloabraham rnaoxidationinchromatinmodificationanddnadamageresponsefollowingexposuretoformaldehyde AT leahildebrandtruiz rnaoxidationinchromatinmodificationanddnadamageresponsefollowingexposuretoformaldehyde AT lydiamcontreras rnaoxidationinchromatinmodificationanddnadamageresponsefollowingexposuretoformaldehyde
_version_	1718377818480443392

RNA oxidation in chromatin modification and DNA-damage response following exposure to formaldehyde

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