Genomic association and further characterisation of faecal immunoglobulin A deficiency in German Shepherd dogs

Abstract Background Immunoglobulin A (IgA) deficiency, chronic enteropathies and exocrine pancreatic insufficiency (EPI) have a high prevalence in German Shepherd dogs (GSD). This prospective study determined the prevalence of faecal IgA deficiency (IgAD) in GSD and investigated several candidate ge...

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Autores principales:	Niels Grützner, Romy M. Heilmann, Ursula Tress, Iain R. Peters, Jan S. Suchodolski, Jörg M. Steiner
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	calgranulin canine trypsin‐like immunoreactivity faecal IgA German Shepherd dog intestine Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/a6b892a429d04bf3946a633c929023d6
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spelling	oai:doaj.org-article:a6b892a429d04bf3946a633c929023d62021-11-19T17:14:25ZGenomic association and further characterisation of faecal immunoglobulin A deficiency in German Shepherd dogs2053-109510.1002/vms3.603https://doaj.org/article/a6b892a429d04bf3946a633c929023d62021-11-01T00:00:00Zhttps://doi.org/10.1002/vms3.603https://doaj.org/toc/2053-1095Abstract Background Immunoglobulin A (IgA) deficiency, chronic enteropathies and exocrine pancreatic insufficiency (EPI) have a high prevalence in German Shepherd dogs (GSD). This prospective study determined the prevalence of faecal IgA deficiency (IgAD) in GSD and investigated several candidate genes and the canine genome for a region or locus co‐segregating with IgAD in GSD. Faecal IgA concentrations were quantified and genomic DNA was extracted from 8 GSD with an undetectable faecal IgA (classified as IgAD) and 80 non‐IgAD GSD. The canine minimal screening set II microsatellite markers were genotyped, with evidence of an association at p < 1.0 × 10−3. Faecal IgA concentrations were also tested for an association with patient clinical and biochemical variables. Results Allele frequencies observed using the candidate gene approach were not associated with faecal IgAD in GSD. In the genome‐wide association study (GWAS), the microsatellite marker FH2361 on canine chromosome 33 approached statistical significance for a link with IgAD in GSD (p = 1.2 × 10−3). A subsequent GWAS in 11 GSD with EPI and 80 control GSD revealed a significant association between EPI and FH2361 (p = 8.2 × 10−4). Conclusions The lack of an association with the phenotype of faecal IgAD in GSD using the candidate gene approach and GWAS might suggests that faecal IgAD in GSD is a relative or transient state of deficiency. However, the prevalence of faecal IgAD in GSD appears to be low (<3%). The relationship between faecal IgAD, EPI and loci close to FH2361 on canine chromosome 33 in GSD warrants further investigation.Niels GrütznerRomy M. HeilmannUrsula TressIain R. PetersJan S. SuchodolskiJörg M. SteinerWileyarticlecalgranulincanine trypsin‐like immunoreactivityfaecal IgAGerman Shepherd dogintestineVeterinary medicineSF600-1100ENVeterinary Medicine and Science, Vol 7, Iss 6, Pp 2144-2155 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	calgranulin canine trypsin‐like immunoreactivity faecal IgA German Shepherd dog intestine Veterinary medicine SF600-1100
spellingShingle	calgranulin canine trypsin‐like immunoreactivity faecal IgA German Shepherd dog intestine Veterinary medicine SF600-1100 Niels Grützner Romy M. Heilmann Ursula Tress Iain R. Peters Jan S. Suchodolski Jörg M. Steiner Genomic association and further characterisation of faecal immunoglobulin A deficiency in German Shepherd dogs
description	Abstract Background Immunoglobulin A (IgA) deficiency, chronic enteropathies and exocrine pancreatic insufficiency (EPI) have a high prevalence in German Shepherd dogs (GSD). This prospective study determined the prevalence of faecal IgA deficiency (IgAD) in GSD and investigated several candidate genes and the canine genome for a region or locus co‐segregating with IgAD in GSD. Faecal IgA concentrations were quantified and genomic DNA was extracted from 8 GSD with an undetectable faecal IgA (classified as IgAD) and 80 non‐IgAD GSD. The canine minimal screening set II microsatellite markers were genotyped, with evidence of an association at p < 1.0 × 10−3. Faecal IgA concentrations were also tested for an association with patient clinical and biochemical variables. Results Allele frequencies observed using the candidate gene approach were not associated with faecal IgAD in GSD. In the genome‐wide association study (GWAS), the microsatellite marker FH2361 on canine chromosome 33 approached statistical significance for a link with IgAD in GSD (p = 1.2 × 10−3). A subsequent GWAS in 11 GSD with EPI and 80 control GSD revealed a significant association between EPI and FH2361 (p = 8.2 × 10−4). Conclusions The lack of an association with the phenotype of faecal IgAD in GSD using the candidate gene approach and GWAS might suggests that faecal IgAD in GSD is a relative or transient state of deficiency. However, the prevalence of faecal IgAD in GSD appears to be low (<3%). The relationship between faecal IgAD, EPI and loci close to FH2361 on canine chromosome 33 in GSD warrants further investigation.
format	article
author	Niels Grützner Romy M. Heilmann Ursula Tress Iain R. Peters Jan S. Suchodolski Jörg M. Steiner
author_facet	Niels Grützner Romy M. Heilmann Ursula Tress Iain R. Peters Jan S. Suchodolski Jörg M. Steiner
author_sort	Niels Grützner
title	Genomic association and further characterisation of faecal immunoglobulin A deficiency in German Shepherd dogs
title_short	Genomic association and further characterisation of faecal immunoglobulin A deficiency in German Shepherd dogs
title_full	Genomic association and further characterisation of faecal immunoglobulin A deficiency in German Shepherd dogs
title_fullStr	Genomic association and further characterisation of faecal immunoglobulin A deficiency in German Shepherd dogs
title_full_unstemmed	Genomic association and further characterisation of faecal immunoglobulin A deficiency in German Shepherd dogs
title_sort	genomic association and further characterisation of faecal immunoglobulin a deficiency in german shepherd dogs
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/a6b892a429d04bf3946a633c929023d6
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Genomic association and further characterisation of faecal immunoglobulin A deficiency in German Shepherd dogs

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