Study of Antibacterial and Anticancer Properties of bioAgNPs Synthesized Using <i>Streptomyces</i> sp. PBD-311B and the Application of bioAgNP-CNC/Alg as an Antibacterial Hydrogel Film against <i>P. aeruginosa</i> USM-AR2 and MRSA

Here, we report the extracellular biosynthesis of silver nanoparticles (AgNPs) and determination of their antibacterial and anticancer properties. We also explore the efficacy of bioAgNPs incorporated in cellulose nanocrystals (CNCs) and alginate (Alg) for the formation of an antibacterial hydrogel...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Hemalatha Murugaiah, Chow Lun Teh, Kai Chew Loh, Ahmad Ramli Mohamad Yahya, Nur Asshifa Md Noh, Noor Hana Hanif Abu Bakar, Daruliza Kernain, Rokiah Hashim, Yazmin Bustami
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	silver nanoparticles <i>Streptomyces</i> sp. biosynthesis antibacterial anticancer cellulose nanocrystals Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/a6bc46aaf4864a799edac219e7a571b8
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a6bc46aaf4864a799edac219e7a571b8
record_format	dspace
spelling	oai:doaj.org-article:a6bc46aaf4864a799edac219e7a571b82021-11-11T18:25:59ZStudy of Antibacterial and Anticancer Properties of bioAgNPs Synthesized Using <i>Streptomyces</i> sp. PBD-311B and the Application of bioAgNP-CNC/Alg as an Antibacterial Hydrogel Film against <i>P. aeruginosa</i> USM-AR2 and MRSA10.3390/molecules262164141420-3049https://doaj.org/article/a6bc46aaf4864a799edac219e7a571b82021-10-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/21/6414https://doaj.org/toc/1420-3049Here, we report the extracellular biosynthesis of silver nanoparticles (AgNPs) and determination of their antibacterial and anticancer properties. We also explore the efficacy of bioAgNPs incorporated in cellulose nanocrystals (CNCs) and alginate (Alg) for the formation of an antibacterial hydrogel film. <i>Streptomyces</i> sp. PBD-311B was used for the biosynthesis of AgNPs. The synthesized bioAgNPs were characterized using UV-Vis spectroscopy, TEM, XRD, and FTIR analysis. Then, the bioAgNPs’ antibacterial and anticancer properties were determined using TEMA and cytotoxicity analysis. To form the antibacterial hydrogel film, bioAgNPs were mixed with a CNC and Alg solution and further characterized using FTIR analysis and a disc diffusion test. The average size of the synthesized bioAgNPs is around 69 ± 2 nm with a spherical shape. XRD analysis confirmed the formation of silver nanocrystals. FTIR analysis showed the presence of protein capping at the bioAgNP surface and could be attributed to the extracellular protein binding to bioAgNPs. The MIC value of bioAgNPs against <i>P. aeruginosa</i> USM-AR2 and MRSA was 6.25 mg/mL and 3.13 mg/mL, respectively. In addition, the bioAgNPs displayed cytotoxicity effects against cancer cells (DBTRG-0.5MG and MCF-7) and showed minimal effects against normal cells (SVG-p12 and MCF-10A), conferring selective toxicity. Interestingly, the bioAgNPs still exhibited inhibition activity when incorporated into CNC/Alg, which implies that the hydrogel film has antibacterial properties. It was also found that bioAgNP-CNC/Alg displayed a minimal or slow release of bioAgNPs owing to the intermolecular interaction and the hydrogel’s properties. Overall, bioAgNP-CNC/Alg is a promising antibacterial hydrogel film that showed inhibition against the pathogenic bacteria <i>P. aeruginosa</i> and MRSA and its application can be further evaluated for the inhibition of cancer cells. It showed benefits for surgical resection of a tumor to avoid post-operative wound infection and tumor recurrence at the surgical site.Hemalatha MurugaiahChow Lun TehKai Chew LohAhmad Ramli Mohamad YahyaNur Asshifa Md NohNoor Hana Hanif Abu BakarDaruliza KernainRokiah HashimYazmin BustamiMDPI AGarticlesilver nanoparticles<i>Streptomyces</i> sp.biosynthesisantibacterialanticancercellulose nanocrystalsOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 6414, p 6414 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	silver nanoparticles <i>Streptomyces</i> sp. biosynthesis antibacterial anticancer cellulose nanocrystals Organic chemistry QD241-441
spellingShingle	silver nanoparticles <i>Streptomyces</i> sp. biosynthesis antibacterial anticancer cellulose nanocrystals Organic chemistry QD241-441 Hemalatha Murugaiah Chow Lun Teh Kai Chew Loh Ahmad Ramli Mohamad Yahya Nur Asshifa Md Noh Noor Hana Hanif Abu Bakar Daruliza Kernain Rokiah Hashim Yazmin Bustami Study of Antibacterial and Anticancer Properties of bioAgNPs Synthesized Using <i>Streptomyces</i> sp. PBD-311B and the Application of bioAgNP-CNC/Alg as an Antibacterial Hydrogel Film against <i>P. aeruginosa</i> USM-AR2 and MRSA
description	Here, we report the extracellular biosynthesis of silver nanoparticles (AgNPs) and determination of their antibacterial and anticancer properties. We also explore the efficacy of bioAgNPs incorporated in cellulose nanocrystals (CNCs) and alginate (Alg) for the formation of an antibacterial hydrogel film. <i>Streptomyces</i> sp. PBD-311B was used for the biosynthesis of AgNPs. The synthesized bioAgNPs were characterized using UV-Vis spectroscopy, TEM, XRD, and FTIR analysis. Then, the bioAgNPs’ antibacterial and anticancer properties were determined using TEMA and cytotoxicity analysis. To form the antibacterial hydrogel film, bioAgNPs were mixed with a CNC and Alg solution and further characterized using FTIR analysis and a disc diffusion test. The average size of the synthesized bioAgNPs is around 69 ± 2 nm with a spherical shape. XRD analysis confirmed the formation of silver nanocrystals. FTIR analysis showed the presence of protein capping at the bioAgNP surface and could be attributed to the extracellular protein binding to bioAgNPs. The MIC value of bioAgNPs against <i>P. aeruginosa</i> USM-AR2 and MRSA was 6.25 mg/mL and 3.13 mg/mL, respectively. In addition, the bioAgNPs displayed cytotoxicity effects against cancer cells (DBTRG-0.5MG and MCF-7) and showed minimal effects against normal cells (SVG-p12 and MCF-10A), conferring selective toxicity. Interestingly, the bioAgNPs still exhibited inhibition activity when incorporated into CNC/Alg, which implies that the hydrogel film has antibacterial properties. It was also found that bioAgNP-CNC/Alg displayed a minimal or slow release of bioAgNPs owing to the intermolecular interaction and the hydrogel’s properties. Overall, bioAgNP-CNC/Alg is a promising antibacterial hydrogel film that showed inhibition against the pathogenic bacteria <i>P. aeruginosa</i> and MRSA and its application can be further evaluated for the inhibition of cancer cells. It showed benefits for surgical resection of a tumor to avoid post-operative wound infection and tumor recurrence at the surgical site.
format	article
author	Hemalatha Murugaiah Chow Lun Teh Kai Chew Loh Ahmad Ramli Mohamad Yahya Nur Asshifa Md Noh Noor Hana Hanif Abu Bakar Daruliza Kernain Rokiah Hashim Yazmin Bustami
author_facet	Hemalatha Murugaiah Chow Lun Teh Kai Chew Loh Ahmad Ramli Mohamad Yahya Nur Asshifa Md Noh Noor Hana Hanif Abu Bakar Daruliza Kernain Rokiah Hashim Yazmin Bustami
author_sort	Hemalatha Murugaiah
title	Study of Antibacterial and Anticancer Properties of bioAgNPs Synthesized Using <i>Streptomyces</i> sp. PBD-311B and the Application of bioAgNP-CNC/Alg as an Antibacterial Hydrogel Film against <i>P. aeruginosa</i> USM-AR2 and MRSA
title_short	Study of Antibacterial and Anticancer Properties of bioAgNPs Synthesized Using <i>Streptomyces</i> sp. PBD-311B and the Application of bioAgNP-CNC/Alg as an Antibacterial Hydrogel Film against <i>P. aeruginosa</i> USM-AR2 and MRSA
title_full	Study of Antibacterial and Anticancer Properties of bioAgNPs Synthesized Using <i>Streptomyces</i> sp. PBD-311B and the Application of bioAgNP-CNC/Alg as an Antibacterial Hydrogel Film against <i>P. aeruginosa</i> USM-AR2 and MRSA
title_fullStr	Study of Antibacterial and Anticancer Properties of bioAgNPs Synthesized Using <i>Streptomyces</i> sp. PBD-311B and the Application of bioAgNP-CNC/Alg as an Antibacterial Hydrogel Film against <i>P. aeruginosa</i> USM-AR2 and MRSA
title_full_unstemmed	Study of Antibacterial and Anticancer Properties of bioAgNPs Synthesized Using <i>Streptomyces</i> sp. PBD-311B and the Application of bioAgNP-CNC/Alg as an Antibacterial Hydrogel Film against <i>P. aeruginosa</i> USM-AR2 and MRSA
title_sort	study of antibacterial and anticancer properties of bioagnps synthesized using <i>streptomyces</i> sp. pbd-311b and the application of bioagnp-cnc/alg as an antibacterial hydrogel film against <i>p. aeruginosa</i> usm-ar2 and mrsa
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/a6bc46aaf4864a799edac219e7a571b8
work_keys_str_mv	AT hemalathamurugaiah studyofantibacterialandanticancerpropertiesofbioagnpssynthesizedusingistreptomycesisppbd311bandtheapplicationofbioagnpcncalgasanantibacterialhydrogelfilmagainstipaeruginosaiusmar2andmrsa AT chowlunteh studyofantibacterialandanticancerpropertiesofbioagnpssynthesizedusingistreptomycesisppbd311bandtheapplicationofbioagnpcncalgasanantibacterialhydrogelfilmagainstipaeruginosaiusmar2andmrsa AT kaichewloh studyofantibacterialandanticancerpropertiesofbioagnpssynthesizedusingistreptomycesisppbd311bandtheapplicationofbioagnpcncalgasanantibacterialhydrogelfilmagainstipaeruginosaiusmar2andmrsa AT ahmadramlimohamadyahya studyofantibacterialandanticancerpropertiesofbioagnpssynthesizedusingistreptomycesisppbd311bandtheapplicationofbioagnpcncalgasanantibacterialhydrogelfilmagainstipaeruginosaiusmar2andmrsa AT nurasshifamdnoh studyofantibacterialandanticancerpropertiesofbioagnpssynthesizedusingistreptomycesisppbd311bandtheapplicationofbioagnpcncalgasanantibacterialhydrogelfilmagainstipaeruginosaiusmar2andmrsa AT noorhanahanifabubakar studyofantibacterialandanticancerpropertiesofbioagnpssynthesizedusingistreptomycesisppbd311bandtheapplicationofbioagnpcncalgasanantibacterialhydrogelfilmagainstipaeruginosaiusmar2andmrsa AT darulizakernain studyofantibacterialandanticancerpropertiesofbioagnpssynthesizedusingistreptomycesisppbd311bandtheapplicationofbioagnpcncalgasanantibacterialhydrogelfilmagainstipaeruginosaiusmar2andmrsa AT rokiahhashim studyofantibacterialandanticancerpropertiesofbioagnpssynthesizedusingistreptomycesisppbd311bandtheapplicationofbioagnpcncalgasanantibacterialhydrogelfilmagainstipaeruginosaiusmar2andmrsa AT yazminbustami studyofantibacterialandanticancerpropertiesofbioagnpssynthesizedusingistreptomycesisppbd311bandtheapplicationofbioagnpcncalgasanantibacterialhydrogelfilmagainstipaeruginosaiusmar2andmrsa
_version_	1718431842198093824

Study of Antibacterial and Anticancer Properties of bioAgNPs Synthesized Using <i>Streptomyces</i> sp. PBD-311B and the Application of bioAgNP-CNC/Alg as an Antibacterial Hydrogel Film against <i>P. aeruginosa</i> USM-AR2 and MRSA

Ejemplares similares