Plk1 bound to Bub1 contributes to spindle assembly checkpoint activity during mitosis

Abstract For faithful chromosome segregation, the formation of stable kinetochore–microtubule attachment and its monitoring by the spindle assembly checkpoint (SAC) are coordinately regulated by mechanisms that are currently ill-defined. Here, we show that polo-like kinase 1 (Plk1), which is instrum...

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Autores principales:	Masanori Ikeda, Kozo Tanaka
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a6bc99e2b9d1486c985b8a8f2a6afb60
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spelling	oai:doaj.org-article:a6bc99e2b9d1486c985b8a8f2a6afb602021-12-02T11:40:33ZPlk1 bound to Bub1 contributes to spindle assembly checkpoint activity during mitosis10.1038/s41598-017-09114-32045-2322https://doaj.org/article/a6bc99e2b9d1486c985b8a8f2a6afb602017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-09114-3https://doaj.org/toc/2045-2322Abstract For faithful chromosome segregation, the formation of stable kinetochore–microtubule attachment and its monitoring by the spindle assembly checkpoint (SAC) are coordinately regulated by mechanisms that are currently ill-defined. Here, we show that polo-like kinase 1 (Plk1), which is instrumental in forming stable kinetochore–microtubule attachments, is also involved in the maintenance of SAC activity by binding to Bub1, but not by binding to CLASP2 or CLIP-170. The effect of Plk1 on the SAC was found to be mediated through phosphorylation of Mps1, an essential kinase for the SAC, as well as through phosphorylation of the MELT repeats in Knl1. Bub1 acts as a platform for assembling other SAC components on the phosphorylated MELT repeats. We propose that Bub1-bound Plk1 is important for the maintenance of SAC activity by supporting Bub1 localization to kinetochores in prometaphase, a time when the kinetochore Mps1 level is reduced, until the formation of stable kinetochore-microtubule attachment is completed. Our study reveals an intricate mechanism for coordinating the formation of stable kinetochore–microtubule attachment and SAC activity.Masanori IkedaKozo TanakaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Masanori Ikeda Kozo Tanaka Plk1 bound to Bub1 contributes to spindle assembly checkpoint activity during mitosis
description	Abstract For faithful chromosome segregation, the formation of stable kinetochore–microtubule attachment and its monitoring by the spindle assembly checkpoint (SAC) are coordinately regulated by mechanisms that are currently ill-defined. Here, we show that polo-like kinase 1 (Plk1), which is instrumental in forming stable kinetochore–microtubule attachments, is also involved in the maintenance of SAC activity by binding to Bub1, but not by binding to CLASP2 or CLIP-170. The effect of Plk1 on the SAC was found to be mediated through phosphorylation of Mps1, an essential kinase for the SAC, as well as through phosphorylation of the MELT repeats in Knl1. Bub1 acts as a platform for assembling other SAC components on the phosphorylated MELT repeats. We propose that Bub1-bound Plk1 is important for the maintenance of SAC activity by supporting Bub1 localization to kinetochores in prometaphase, a time when the kinetochore Mps1 level is reduced, until the formation of stable kinetochore-microtubule attachment is completed. Our study reveals an intricate mechanism for coordinating the formation of stable kinetochore–microtubule attachment and SAC activity.
format	article
author	Masanori Ikeda Kozo Tanaka
author_facet	Masanori Ikeda Kozo Tanaka
author_sort	Masanori Ikeda
title	Plk1 bound to Bub1 contributes to spindle assembly checkpoint activity during mitosis
title_short	Plk1 bound to Bub1 contributes to spindle assembly checkpoint activity during mitosis
title_full	Plk1 bound to Bub1 contributes to spindle assembly checkpoint activity during mitosis
title_fullStr	Plk1 bound to Bub1 contributes to spindle assembly checkpoint activity during mitosis
title_full_unstemmed	Plk1 bound to Bub1 contributes to spindle assembly checkpoint activity during mitosis
title_sort	plk1 bound to bub1 contributes to spindle assembly checkpoint activity during mitosis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/a6bc99e2b9d1486c985b8a8f2a6afb60
work_keys_str_mv	AT masanoriikeda plk1boundtobub1contributestospindleassemblycheckpointactivityduringmitosis AT kozotanaka plk1boundtobub1contributestospindleassemblycheckpointactivityduringmitosis
_version_	1718395600104325120

Plk1 bound to Bub1 contributes to spindle assembly checkpoint activity during mitosis

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