Comparative Genomic Characterization of Buffalo Fibronectin Type III Domain Proteins: Exploring the Novel Role of FNDC5/Irisin as a Ligand of Gonadal Receptors
FN-III proteins are widely distributed in mammals and are usually involved in cellular growth, differentiation, and adhesion. The FNDC5/irisin regulates energy metabolism and is present in different tissues (liver, brain, etc.). The present study aimed to investigate the physiochemical characteristi...
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2021
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oai:doaj.org-article:a6c2df83d6d6415a89da0b83a64ba6282021-11-25T16:48:06ZComparative Genomic Characterization of Buffalo Fibronectin Type III Domain Proteins: Exploring the Novel Role of FNDC5/Irisin as a Ligand of Gonadal Receptors10.3390/biology101112072079-7737https://doaj.org/article/a6c2df83d6d6415a89da0b83a64ba6282021-11-01T00:00:00Zhttps://www.mdpi.com/2079-7737/10/11/1207https://doaj.org/toc/2079-7737FN-III proteins are widely distributed in mammals and are usually involved in cellular growth, differentiation, and adhesion. The FNDC5/irisin regulates energy metabolism and is present in different tissues (liver, brain, etc.). The present study aimed to investigate the physiochemical characteristics and the evolution of FN-III proteins and FNDC5/irisin as a ligand targeting the gonadal receptors including androgen (AR), DDB1 and CUL4 associated factor 6 (DCAF6), estrogen-related receptor β (ERR-β), estrogen-related receptor γ (ERR-γ), Krüppel-like factor 15 (KLF15), and nuclear receptor subfamily 3 group C member 1 (NR3C1). Moreover, the putative role of irisin in folliculogenesis and spermatogenesis was also elucidated. We presented the molecular structure and function of 29 <i>FN-III</i> genes widely distributed in the buffalo genome. Phylogenetic analysis, motif, and conserved domain pattern demonstrated the evolutionary well-conserved nature of FN-III proteins with a variety of stable to unstable, hydrophobic to hydrophilic, and thermostable to thermo-unstable properties. The comparative structural configuration of FNDC5 revealed amino acid variations but still the FNDC5 structure of humans, buffalo, and cattle was quite similar to each other. For the first time, we predicted the binding scores and interface residues of FNDC5/irisin as a ligand for six representative receptors having a functional role in energy homeostasis, and a significant involvement in folliculogenesis and spermatogenesis in buffalo.Siwen WuFaiz-ul HassanYuhong LuoIsrar FatimaIshtiaq AhmedAwais IhsanWarda SafdarQingyou LiuSaif ur RehmanMDPI AGarticlebuffaloFN-III proteinsFNDC5/irisinreceptorsbinding affinitiesBiology (General)QH301-705.5ENBiology, Vol 10, Iss 1207, p 1207 (2021) |
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| language |
EN |
| topic |
buffalo FN-III proteins FNDC5/irisin receptors binding affinities Biology (General) QH301-705.5 |
| spellingShingle |
buffalo FN-III proteins FNDC5/irisin receptors binding affinities Biology (General) QH301-705.5 Siwen Wu Faiz-ul Hassan Yuhong Luo Israr Fatima Ishtiaq Ahmed Awais Ihsan Warda Safdar Qingyou Liu Saif ur Rehman Comparative Genomic Characterization of Buffalo Fibronectin Type III Domain Proteins: Exploring the Novel Role of FNDC5/Irisin as a Ligand of Gonadal Receptors |
| description |
FN-III proteins are widely distributed in mammals and are usually involved in cellular growth, differentiation, and adhesion. The FNDC5/irisin regulates energy metabolism and is present in different tissues (liver, brain, etc.). The present study aimed to investigate the physiochemical characteristics and the evolution of FN-III proteins and FNDC5/irisin as a ligand targeting the gonadal receptors including androgen (AR), DDB1 and CUL4 associated factor 6 (DCAF6), estrogen-related receptor β (ERR-β), estrogen-related receptor γ (ERR-γ), Krüppel-like factor 15 (KLF15), and nuclear receptor subfamily 3 group C member 1 (NR3C1). Moreover, the putative role of irisin in folliculogenesis and spermatogenesis was also elucidated. We presented the molecular structure and function of 29 <i>FN-III</i> genes widely distributed in the buffalo genome. Phylogenetic analysis, motif, and conserved domain pattern demonstrated the evolutionary well-conserved nature of FN-III proteins with a variety of stable to unstable, hydrophobic to hydrophilic, and thermostable to thermo-unstable properties. The comparative structural configuration of FNDC5 revealed amino acid variations but still the FNDC5 structure of humans, buffalo, and cattle was quite similar to each other. For the first time, we predicted the binding scores and interface residues of FNDC5/irisin as a ligand for six representative receptors having a functional role in energy homeostasis, and a significant involvement in folliculogenesis and spermatogenesis in buffalo. |
| format |
article |
| author |
Siwen Wu Faiz-ul Hassan Yuhong Luo Israr Fatima Ishtiaq Ahmed Awais Ihsan Warda Safdar Qingyou Liu Saif ur Rehman |
| author_facet |
Siwen Wu Faiz-ul Hassan Yuhong Luo Israr Fatima Ishtiaq Ahmed Awais Ihsan Warda Safdar Qingyou Liu Saif ur Rehman |
| author_sort |
Siwen Wu |
| title |
Comparative Genomic Characterization of Buffalo Fibronectin Type III Domain Proteins: Exploring the Novel Role of FNDC5/Irisin as a Ligand of Gonadal Receptors |
| title_short |
Comparative Genomic Characterization of Buffalo Fibronectin Type III Domain Proteins: Exploring the Novel Role of FNDC5/Irisin as a Ligand of Gonadal Receptors |
| title_full |
Comparative Genomic Characterization of Buffalo Fibronectin Type III Domain Proteins: Exploring the Novel Role of FNDC5/Irisin as a Ligand of Gonadal Receptors |
| title_fullStr |
Comparative Genomic Characterization of Buffalo Fibronectin Type III Domain Proteins: Exploring the Novel Role of FNDC5/Irisin as a Ligand of Gonadal Receptors |
| title_full_unstemmed |
Comparative Genomic Characterization of Buffalo Fibronectin Type III Domain Proteins: Exploring the Novel Role of FNDC5/Irisin as a Ligand of Gonadal Receptors |
| title_sort |
comparative genomic characterization of buffalo fibronectin type iii domain proteins: exploring the novel role of fndc5/irisin as a ligand of gonadal receptors |
| publisher |
MDPI AG |
| publishDate |
2021 |
| url |
https://doaj.org/article/a6c2df83d6d6415a89da0b83a64ba628 |
| work_keys_str_mv |
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| _version_ |
1718412975207874560 |