Label-Free Flow Multiplex Biosensing via Photonic Crystal Surface Mode Detection

Abstract Circulating cancer markers are metabolic products found in body fluids of cancer patients, which are specific for a certain type of malignant tumors. Cancer marker detection plays a key role in cancer diagnosis, treatment, and disease monitoring. The growing need for early cancer diagnosis...

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Autores principales:	Irina Petrova, Valery Konopsky, Igor Nabiev, Alyona Sukhanova
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a6c3ea36148b49839cbce6983c9111cb
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spelling	oai:doaj.org-article:a6c3ea36148b49839cbce6983c9111cb2021-12-02T15:08:48ZLabel-Free Flow Multiplex Biosensing via Photonic Crystal Surface Mode Detection10.1038/s41598-019-45166-32045-2322https://doaj.org/article/a6c3ea36148b49839cbce6983c9111cb2019-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-45166-3https://doaj.org/toc/2045-2322Abstract Circulating cancer markers are metabolic products found in body fluids of cancer patients, which are specific for a certain type of malignant tumors. Cancer marker detection plays a key role in cancer diagnosis, treatment, and disease monitoring. The growing need for early cancer diagnosis requires quick and sensitive analytical approaches to detection of cancer markers. The approach based on the photonic crystal surface mode (PC SM) detection has been developed as a label-free high-precision biosensing technique. It allows real-time monitoring of molecular and cellular interactions using independent recording of the total internal reflection angle and the excitation angle of the PC surface wave. We used the PC SM technique for simultaneous detection of the ovarian cancer marker cancer antigen 125 and two breast cancer markers, human epidermal growth factor receptor 2 and cancer antigen 15-3. The new assay is based on the real-time flow detection of specific interaction between the antigens and capture antibodies. Its particular advantage is the possibility of multichannel recording with the same chip, which can be used for multiplexed detection of several cancer markers in a single experiment. The developed approach demonstrates high specificity and sensitivity for detection of all three biomarkers.Irina PetrovaValery KonopskyIgor NabievAlyona SukhanovaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-9 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Irina Petrova Valery Konopsky Igor Nabiev Alyona Sukhanova Label-Free Flow Multiplex Biosensing via Photonic Crystal Surface Mode Detection
description	Abstract Circulating cancer markers are metabolic products found in body fluids of cancer patients, which are specific for a certain type of malignant tumors. Cancer marker detection plays a key role in cancer diagnosis, treatment, and disease monitoring. The growing need for early cancer diagnosis requires quick and sensitive analytical approaches to detection of cancer markers. The approach based on the photonic crystal surface mode (PC SM) detection has been developed as a label-free high-precision biosensing technique. It allows real-time monitoring of molecular and cellular interactions using independent recording of the total internal reflection angle and the excitation angle of the PC surface wave. We used the PC SM technique for simultaneous detection of the ovarian cancer marker cancer antigen 125 and two breast cancer markers, human epidermal growth factor receptor 2 and cancer antigen 15-3. The new assay is based on the real-time flow detection of specific interaction between the antigens and capture antibodies. Its particular advantage is the possibility of multichannel recording with the same chip, which can be used for multiplexed detection of several cancer markers in a single experiment. The developed approach demonstrates high specificity and sensitivity for detection of all three biomarkers.
format	article
author	Irina Petrova Valery Konopsky Igor Nabiev Alyona Sukhanova
author_facet	Irina Petrova Valery Konopsky Igor Nabiev Alyona Sukhanova
author_sort	Irina Petrova
title	Label-Free Flow Multiplex Biosensing via Photonic Crystal Surface Mode Detection
title_short	Label-Free Flow Multiplex Biosensing via Photonic Crystal Surface Mode Detection
title_full	Label-Free Flow Multiplex Biosensing via Photonic Crystal Surface Mode Detection
title_fullStr	Label-Free Flow Multiplex Biosensing via Photonic Crystal Surface Mode Detection
title_full_unstemmed	Label-Free Flow Multiplex Biosensing via Photonic Crystal Surface Mode Detection
title_sort	label-free flow multiplex biosensing via photonic crystal surface mode detection
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/a6c3ea36148b49839cbce6983c9111cb
work_keys_str_mv	AT irinapetrova labelfreeflowmultiplexbiosensingviaphotoniccrystalsurfacemodedetection AT valerykonopsky labelfreeflowmultiplexbiosensingviaphotoniccrystalsurfacemodedetection AT igornabiev labelfreeflowmultiplexbiosensingviaphotoniccrystalsurfacemodedetection AT alyonasukhanova labelfreeflowmultiplexbiosensingviaphotoniccrystalsurfacemodedetection
_version_	1718388018902990848

Label-Free Flow Multiplex Biosensing via Photonic Crystal Surface Mode Detection

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