MiR-339-5p regulates the growth, colony formation and metastasis of colorectal cancer cells by targeting PRL-1.

MicroRNAs (miRNAs) have been suggested to play a vital role in regulate tumor progression and invasion. However, the expression of miR-339-5p in colorectal cancer and its effects are not known. Here, we report that miR-339-5p is a tumor suppressor by regulating expression of PRL-1. In this study, we...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Chang Zhou, Guobing Liu, Lijing Wang, Yanxia Lu, Li Yuan, Lin Zheng, Fang Chen, Fanli Peng, Xuenong Li
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a6cef5073086479a827f3e175e548a6b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a6cef5073086479a827f3e175e548a6b
record_format	dspace
spelling	oai:doaj.org-article:a6cef5073086479a827f3e175e548a6b2021-11-18T07:45:32ZMiR-339-5p regulates the growth, colony formation and metastasis of colorectal cancer cells by targeting PRL-1.1932-620310.1371/journal.pone.0063142https://doaj.org/article/a6cef5073086479a827f3e175e548a6b2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23696794/?tool=EBIhttps://doaj.org/toc/1932-6203MicroRNAs (miRNAs) have been suggested to play a vital role in regulate tumor progression and invasion. However, the expression of miR-339-5p in colorectal cancer and its effects are not known. Here, we report that miR-339-5p is a tumor suppressor by regulating expression of PRL-1. In this study, we showed that downregulated miR-339-5p levels in colorectal cancer tissues and highly invasive CRC cell lines. Furthermore, enhancing the expression of miR-339-5p inhibited CRC cell growth, migration and invasion in vitro and suppressed tumor growth in vivo. We then screened and identified a novel miR-339-5p target, phosphatases of regenerating liver-1 1 (PRL-1), and it was further confirmed by luciferase assay. Overexpression of miR-339-5p would also reduce the expression of PRL-1 mRNA and protein. The reduced PRL-1 expression was associated with low expression of phosphorylated-extracellular signal-regulated kinase1/2 (p-ERK1/2). Conversely, reduction of miR-339-5p by inhibitors in cells stimulated these phenotypes. In conclusion, our results demonstrate that miR-339-5p functions as a tumor suppressor and plays a role in inhibiting growth and metastasis of CRC cells through targeting PRL-1 and regulating p-ERK1/2 .These findings suggest that miR-339-5p may be useful as a new potential therapeutic target for CRC.Chang ZhouGuobing LiuLijing WangYanxia LuLi YuanLin ZhengFang ChenFanli PengXuenong LiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 5, p e63142 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chang Zhou Guobing Liu Lijing Wang Yanxia Lu Li Yuan Lin Zheng Fang Chen Fanli Peng Xuenong Li MiR-339-5p regulates the growth, colony formation and metastasis of colorectal cancer cells by targeting PRL-1.
description	MicroRNAs (miRNAs) have been suggested to play a vital role in regulate tumor progression and invasion. However, the expression of miR-339-5p in colorectal cancer and its effects are not known. Here, we report that miR-339-5p is a tumor suppressor by regulating expression of PRL-1. In this study, we showed that downregulated miR-339-5p levels in colorectal cancer tissues and highly invasive CRC cell lines. Furthermore, enhancing the expression of miR-339-5p inhibited CRC cell growth, migration and invasion in vitro and suppressed tumor growth in vivo. We then screened and identified a novel miR-339-5p target, phosphatases of regenerating liver-1 1 (PRL-1), and it was further confirmed by luciferase assay. Overexpression of miR-339-5p would also reduce the expression of PRL-1 mRNA and protein. The reduced PRL-1 expression was associated with low expression of phosphorylated-extracellular signal-regulated kinase1/2 (p-ERK1/2). Conversely, reduction of miR-339-5p by inhibitors in cells stimulated these phenotypes. In conclusion, our results demonstrate that miR-339-5p functions as a tumor suppressor and plays a role in inhibiting growth and metastasis of CRC cells through targeting PRL-1 and regulating p-ERK1/2 .These findings suggest that miR-339-5p may be useful as a new potential therapeutic target for CRC.
format	article
author	Chang Zhou Guobing Liu Lijing Wang Yanxia Lu Li Yuan Lin Zheng Fang Chen Fanli Peng Xuenong Li
author_facet	Chang Zhou Guobing Liu Lijing Wang Yanxia Lu Li Yuan Lin Zheng Fang Chen Fanli Peng Xuenong Li
author_sort	Chang Zhou
title	MiR-339-5p regulates the growth, colony formation and metastasis of colorectal cancer cells by targeting PRL-1.
title_short	MiR-339-5p regulates the growth, colony formation and metastasis of colorectal cancer cells by targeting PRL-1.
title_full	MiR-339-5p regulates the growth, colony formation and metastasis of colorectal cancer cells by targeting PRL-1.
title_fullStr	MiR-339-5p regulates the growth, colony formation and metastasis of colorectal cancer cells by targeting PRL-1.
title_full_unstemmed	MiR-339-5p regulates the growth, colony formation and metastasis of colorectal cancer cells by targeting PRL-1.
title_sort	mir-339-5p regulates the growth, colony formation and metastasis of colorectal cancer cells by targeting prl-1.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/a6cef5073086479a827f3e175e548a6b
work_keys_str_mv	AT changzhou mir3395pregulatesthegrowthcolonyformationandmetastasisofcolorectalcancercellsbytargetingprl1 AT guobingliu mir3395pregulatesthegrowthcolonyformationandmetastasisofcolorectalcancercellsbytargetingprl1 AT lijingwang mir3395pregulatesthegrowthcolonyformationandmetastasisofcolorectalcancercellsbytargetingprl1 AT yanxialu mir3395pregulatesthegrowthcolonyformationandmetastasisofcolorectalcancercellsbytargetingprl1 AT liyuan mir3395pregulatesthegrowthcolonyformationandmetastasisofcolorectalcancercellsbytargetingprl1 AT linzheng mir3395pregulatesthegrowthcolonyformationandmetastasisofcolorectalcancercellsbytargetingprl1 AT fangchen mir3395pregulatesthegrowthcolonyformationandmetastasisofcolorectalcancercellsbytargetingprl1 AT fanlipeng mir3395pregulatesthegrowthcolonyformationandmetastasisofcolorectalcancercellsbytargetingprl1 AT xuenongli mir3395pregulatesthegrowthcolonyformationandmetastasisofcolorectalcancercellsbytargetingprl1
_version_	1718422993004134400

MiR-339-5p regulates the growth, colony formation and metastasis of colorectal cancer cells by targeting PRL-1.

Ejemplares similares