Lipotoxic stress alters the membrane lipid profile of extracellular vesicles released by Huh-7 hepatocarcinoma cells

Abstract Extracellular vesicles (EVs) are well-known mediators in intercellular communication playing pivotal roles in promoting liver inflammation and fibrosis, events associated to hepatic lipotoxicity caused by saturated free fatty acid overloading. However, despite the importance of lipids in EV...

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Autores principales:	Sandra Buratta, Y. Shimanaka, E. Costanzi, S. Ni, L. Urbanelli, N. Kono, F. Morena, K. Sagini, S. Giovagnoli, R. Romani, M. Gargaro, H. Arai, C. Emiliani
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a6d1b94e294d4a239d5ffe634057b60f
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spelling	oai:doaj.org-article:a6d1b94e294d4a239d5ffe634057b60f2021-12-02T15:53:42ZLipotoxic stress alters the membrane lipid profile of extracellular vesicles released by Huh-7 hepatocarcinoma cells10.1038/s41598-021-84268-92045-2322https://doaj.org/article/a6d1b94e294d4a239d5ffe634057b60f2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84268-9https://doaj.org/toc/2045-2322Abstract Extracellular vesicles (EVs) are well-known mediators in intercellular communication playing pivotal roles in promoting liver inflammation and fibrosis, events associated to hepatic lipotoxicity caused by saturated free fatty acid overloading. However, despite the importance of lipids in EV membrane architecture which, in turn, affects EV biophysical and biological properties, little is known about the lipid asset of EVs released under these conditions. Here, we analyzed phospholipid profile alterations of EVs released by hepatocarcinoma Huh-7 cells under increased membrane lipid saturation induced by supplementation with saturated fatty acid palmitate or Δ9 desaturase inhibition, using oleate, a nontoxic monounsaturated fatty acid, as control. As an increase of membrane lipid saturation induces endoplasmic reticulum (ER) stress, we also analyzed phospholipid rearrangements in EVs released by Huh-7 cells treated with thapsigargin, a conventional ER stress inducer. Results demonstrate that lipotoxic and/or ER stress conditions induced rearrangements not only into cell membrane phospholipids but also into the released EVs. Thus, cell membrane saturation level and/or ER stress are crucial to determine which lipids are discarded via EVs and EV lipid cargos might be useful to discriminate hepatic lipid overloading and ER stress.Sandra BurattaY. ShimanakaE. CostanziS. NiL. UrbanelliN. KonoF. MorenaK. SaginiS. GiovagnoliR. RomaniM. GargaroH. AraiC. EmilianiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sandra Buratta Y. Shimanaka E. Costanzi S. Ni L. Urbanelli N. Kono F. Morena K. Sagini S. Giovagnoli R. Romani M. Gargaro H. Arai C. Emiliani Lipotoxic stress alters the membrane lipid profile of extracellular vesicles released by Huh-7 hepatocarcinoma cells
description	Abstract Extracellular vesicles (EVs) are well-known mediators in intercellular communication playing pivotal roles in promoting liver inflammation and fibrosis, events associated to hepatic lipotoxicity caused by saturated free fatty acid overloading. However, despite the importance of lipids in EV membrane architecture which, in turn, affects EV biophysical and biological properties, little is known about the lipid asset of EVs released under these conditions. Here, we analyzed phospholipid profile alterations of EVs released by hepatocarcinoma Huh-7 cells under increased membrane lipid saturation induced by supplementation with saturated fatty acid palmitate or Δ9 desaturase inhibition, using oleate, a nontoxic monounsaturated fatty acid, as control. As an increase of membrane lipid saturation induces endoplasmic reticulum (ER) stress, we also analyzed phospholipid rearrangements in EVs released by Huh-7 cells treated with thapsigargin, a conventional ER stress inducer. Results demonstrate that lipotoxic and/or ER stress conditions induced rearrangements not only into cell membrane phospholipids but also into the released EVs. Thus, cell membrane saturation level and/or ER stress are crucial to determine which lipids are discarded via EVs and EV lipid cargos might be useful to discriminate hepatic lipid overloading and ER stress.
format	article
author	Sandra Buratta Y. Shimanaka E. Costanzi S. Ni L. Urbanelli N. Kono F. Morena K. Sagini S. Giovagnoli R. Romani M. Gargaro H. Arai C. Emiliani
author_facet	Sandra Buratta Y. Shimanaka E. Costanzi S. Ni L. Urbanelli N. Kono F. Morena K. Sagini S. Giovagnoli R. Romani M. Gargaro H. Arai C. Emiliani
author_sort	Sandra Buratta
title	Lipotoxic stress alters the membrane lipid profile of extracellular vesicles released by Huh-7 hepatocarcinoma cells
title_short	Lipotoxic stress alters the membrane lipid profile of extracellular vesicles released by Huh-7 hepatocarcinoma cells
title_full	Lipotoxic stress alters the membrane lipid profile of extracellular vesicles released by Huh-7 hepatocarcinoma cells
title_fullStr	Lipotoxic stress alters the membrane lipid profile of extracellular vesicles released by Huh-7 hepatocarcinoma cells
title_full_unstemmed	Lipotoxic stress alters the membrane lipid profile of extracellular vesicles released by Huh-7 hepatocarcinoma cells
title_sort	lipotoxic stress alters the membrane lipid profile of extracellular vesicles released by huh-7 hepatocarcinoma cells
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/a6d1b94e294d4a239d5ffe634057b60f
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Lipotoxic stress alters the membrane lipid profile of extracellular vesicles released by Huh-7 hepatocarcinoma cells

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