Antimicrobial Resistance and Genomic Characterization of Six New Sequence Types in Multidrug-Resistant Pseudomonas aeruginosa Clinical Isolates from Pakistan

Pseudomonas aeruginosa (P. aeruginosa) is a major bacterial pathogen associated with a variety of infections with high mortality rates. Most of the clinical P. aeruginosa isolates belong to a limited number of genetic subgroups characterized b...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Sidra Irum, Kanwal Naz, Nimat Ullah, Zeeshan Mustafa, Amjad Ali, Muhammad Arslan, Kashaf Khalid, Saadia Andleeb
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Pseudomonas aeruginosa</i> whole-genome sequence new sequence types (STs) antimicrobial resistance multidrug-resistance Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/a6d37b4bd5e74cdc8bbc9232a569743a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a6d37b4bd5e74cdc8bbc9232a569743a
record_format	dspace
spelling	oai:doaj.org-article:a6d37b4bd5e74cdc8bbc9232a569743a2021-11-25T16:24:28ZAntimicrobial Resistance and Genomic Characterization of Six New Sequence Types in Multidrug-Resistant <i>Pseudomonas aeruginosa</i> Clinical Isolates from Pakistan10.3390/antibiotics101113862079-6382https://doaj.org/article/a6d37b4bd5e74cdc8bbc9232a569743a2021-11-01T00:00:00Zhttps://www.mdpi.com/2079-6382/10/11/1386https://doaj.org/toc/2079-6382<i>Pseudomonas aeruginosa</i> (<i>P. aeruginosa</i>) is a major bacterial pathogen associated with a variety of infections with high mortality rates. Most of the clinical <i>P. aeruginosa</i> isolates belong to a limited number of genetic subgroups characterized by multiple housekeeping genes’ sequences (usually 5–7) through the Multi-Locus Sequence Typing (MLST) scheme. The emergence and dissemination of novel multidrug-resistant (MDR) sequence types (ST) in <i>P. aeruginosa</i> pose serious clinical concerns. We performed whole-genome sequencing on a cohort (<i>n</i> = 160) of MDR <i>P. aeruginosa</i> isolates collected from a tertiary care hospital lab in Pakistan and found six isolates belonging to six unique MLST allelic profiles. The genomes were submitted to the PubMLST database and new ST numbers (ST3493, ST3494, ST3472, ST3489, ST3491, and ST3492) were assigned to the respective allele combinations. MLST and core-genome-based phylogenetic analysis confirmed the divergence of these isolates and positioned them in separate branches. Analysis of the resistome of the new STs isolates revealed the presence of genes <i>bla</i>OXA-50, <i>bla</i>PAO, <i>bla</i>PDC, <i>bla</i>VIM-2, <i>aph</i>(3′)-IIb, <i>aac</i>(6′)-II, <i>aac</i>(3)-Id, <i>fos</i>A, <i>cat</i>B7, <i>dfr</i>B2, <i>crp</i>P, <i>mer</i>P and a number of missense and frame-shift mutations in chromosomal genes conferring resistance to various antipseudomonal antibiotics. The <i>exo</i>S, <i>exo</i>T, <i>pvd</i>E, <i>rhl</i>I, <i>rhl</i>R, <i>las</i>A, <i>las</i>B, <i>las</i>I, and <i>las</i>R genes were the most prevalent virulence-related genes among the new ST isolates. The different genotypic features revealed the adaptation of these new clones to a variety of infections by various mutations in genes affecting antimicrobial resistance, quorum sensing and biofilm formation. Close monitoring of these antibiotic-resistant pathogens and surveillance mechanisms needs to be adopted to reduce their spread to the healthcare facilities of Pakistan. We believe that these strains can be used as reference strains for future comparative analysis of isolates belonging to the same STs.Sidra IrumKanwal NazNimat UllahZeeshan MustafaAmjad AliMuhammad ArslanKashaf KhalidSaadia AndleebMDPI AGarticle<i>Pseudomonas aeruginosa</i>whole-genome sequencenew sequence types (STs)antimicrobial resistancemultidrug-resistanceTherapeutics. PharmacologyRM1-950ENAntibiotics, Vol 10, Iss 1386, p 1386 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Pseudomonas aeruginosa</i> whole-genome sequence new sequence types (STs) antimicrobial resistance multidrug-resistance Therapeutics. Pharmacology RM1-950
spellingShingle	<i>Pseudomonas aeruginosa</i> whole-genome sequence new sequence types (STs) antimicrobial resistance multidrug-resistance Therapeutics. Pharmacology RM1-950 Sidra Irum Kanwal Naz Nimat Ullah Zeeshan Mustafa Amjad Ali Muhammad Arslan Kashaf Khalid Saadia Andleeb Antimicrobial Resistance and Genomic Characterization of Six New Sequence Types in Multidrug-Resistant <i>Pseudomonas aeruginosa</i> Clinical Isolates from Pakistan
description	<i>Pseudomonas aeruginosa</i> (<i>P. aeruginosa</i>) is a major bacterial pathogen associated with a variety of infections with high mortality rates. Most of the clinical <i>P. aeruginosa</i> isolates belong to a limited number of genetic subgroups characterized by multiple housekeeping genes’ sequences (usually 5–7) through the Multi-Locus Sequence Typing (MLST) scheme. The emergence and dissemination of novel multidrug-resistant (MDR) sequence types (ST) in <i>P. aeruginosa</i> pose serious clinical concerns. We performed whole-genome sequencing on a cohort (<i>n</i> = 160) of MDR <i>P. aeruginosa</i> isolates collected from a tertiary care hospital lab in Pakistan and found six isolates belonging to six unique MLST allelic profiles. The genomes were submitted to the PubMLST database and new ST numbers (ST3493, ST3494, ST3472, ST3489, ST3491, and ST3492) were assigned to the respective allele combinations. MLST and core-genome-based phylogenetic analysis confirmed the divergence of these isolates and positioned them in separate branches. Analysis of the resistome of the new STs isolates revealed the presence of genes <i>bla</i>OXA-50, <i>bla</i>PAO, <i>bla</i>PDC, <i>bla</i>VIM-2, <i>aph</i>(3′)-IIb, <i>aac</i>(6′)-II, <i>aac</i>(3)-Id, <i>fos</i>A, <i>cat</i>B7, <i>dfr</i>B2, <i>crp</i>P, <i>mer</i>P and a number of missense and frame-shift mutations in chromosomal genes conferring resistance to various antipseudomonal antibiotics. The <i>exo</i>S, <i>exo</i>T, <i>pvd</i>E, <i>rhl</i>I, <i>rhl</i>R, <i>las</i>A, <i>las</i>B, <i>las</i>I, and <i>las</i>R genes were the most prevalent virulence-related genes among the new ST isolates. The different genotypic features revealed the adaptation of these new clones to a variety of infections by various mutations in genes affecting antimicrobial resistance, quorum sensing and biofilm formation. Close monitoring of these antibiotic-resistant pathogens and surveillance mechanisms needs to be adopted to reduce their spread to the healthcare facilities of Pakistan. We believe that these strains can be used as reference strains for future comparative analysis of isolates belonging to the same STs.
format	article
author	Sidra Irum Kanwal Naz Nimat Ullah Zeeshan Mustafa Amjad Ali Muhammad Arslan Kashaf Khalid Saadia Andleeb
author_facet	Sidra Irum Kanwal Naz Nimat Ullah Zeeshan Mustafa Amjad Ali Muhammad Arslan Kashaf Khalid Saadia Andleeb
author_sort	Sidra Irum
title	Antimicrobial Resistance and Genomic Characterization of Six New Sequence Types in Multidrug-Resistant <i>Pseudomonas aeruginosa</i> Clinical Isolates from Pakistan
title_short	Antimicrobial Resistance and Genomic Characterization of Six New Sequence Types in Multidrug-Resistant <i>Pseudomonas aeruginosa</i> Clinical Isolates from Pakistan
title_full	Antimicrobial Resistance and Genomic Characterization of Six New Sequence Types in Multidrug-Resistant <i>Pseudomonas aeruginosa</i> Clinical Isolates from Pakistan
title_fullStr	Antimicrobial Resistance and Genomic Characterization of Six New Sequence Types in Multidrug-Resistant <i>Pseudomonas aeruginosa</i> Clinical Isolates from Pakistan
title_full_unstemmed	Antimicrobial Resistance and Genomic Characterization of Six New Sequence Types in Multidrug-Resistant <i>Pseudomonas aeruginosa</i> Clinical Isolates from Pakistan
title_sort	antimicrobial resistance and genomic characterization of six new sequence types in multidrug-resistant <i>pseudomonas aeruginosa</i> clinical isolates from pakistan
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/a6d37b4bd5e74cdc8bbc9232a569743a
work_keys_str_mv	AT sidrairum antimicrobialresistanceandgenomiccharacterizationofsixnewsequencetypesinmultidrugresistantipseudomonasaeruginosaiclinicalisolatesfrompakistan AT kanwalnaz antimicrobialresistanceandgenomiccharacterizationofsixnewsequencetypesinmultidrugresistantipseudomonasaeruginosaiclinicalisolatesfrompakistan AT nimatullah antimicrobialresistanceandgenomiccharacterizationofsixnewsequencetypesinmultidrugresistantipseudomonasaeruginosaiclinicalisolatesfrompakistan AT zeeshanmustafa antimicrobialresistanceandgenomiccharacterizationofsixnewsequencetypesinmultidrugresistantipseudomonasaeruginosaiclinicalisolatesfrompakistan AT amjadali antimicrobialresistanceandgenomiccharacterizationofsixnewsequencetypesinmultidrugresistantipseudomonasaeruginosaiclinicalisolatesfrompakistan AT muhammadarslan antimicrobialresistanceandgenomiccharacterizationofsixnewsequencetypesinmultidrugresistantipseudomonasaeruginosaiclinicalisolatesfrompakistan AT kashafkhalid antimicrobialresistanceandgenomiccharacterizationofsixnewsequencetypesinmultidrugresistantipseudomonasaeruginosaiclinicalisolatesfrompakistan AT saadiaandleeb antimicrobialresistanceandgenomiccharacterizationofsixnewsequencetypesinmultidrugresistantipseudomonasaeruginosaiclinicalisolatesfrompakistan
_version_	1718413217710997504

Antimicrobial Resistance and Genomic Characterization of Six New Sequence Types in Multidrug-Resistant <i>Pseudomonas aeruginosa</i> Clinical Isolates from Pakistan

Ejemplares similares