Analysis of vascular development in the hydra sterol biosynthetic mutants of Arabidopsis.

<h4>Background</h4>The control of vascular tissue development in plants is influenced by diverse hormonal signals, but their interactions during this process are not well understood. Wild-type sterol profiles are essential for growth, tissue patterning and signalling processes in plant d...

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Autores principales:	Margaret Pullen, Nick Clark, Fatemeh Zarinkamar, Jennifer Topping, Keith Lindsey
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a6d870899ba54f74b238937a1465c555
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spelling	oai:doaj.org-article:a6d870899ba54f74b238937a1465c5552021-12-02T20:11:51ZAnalysis of vascular development in the hydra sterol biosynthetic mutants of Arabidopsis.1932-620310.1371/journal.pone.0012227https://doaj.org/article/a6d870899ba54f74b238937a1465c5552010-08-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20808926/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>The control of vascular tissue development in plants is influenced by diverse hormonal signals, but their interactions during this process are not well understood. Wild-type sterol profiles are essential for growth, tissue patterning and signalling processes in plant development, and are required for regulated vascular patterning.<h4>Methodology/principal findings</h4>Here we investigate the roles of sterols in vascular tissue development, through an analysis of the Arabidopsis mutants hydra1 and fackel/hydra2, which are defective in the enzymes sterol isomerase and sterol C-14 reductase respectively. We show that defective vascular patterning in the shoot is associated with ectopic cell divisions. Expression of the auxin-regulated AtHB8 homeobox gene is disrupted in mutant embryos and seedlings, associated with variably incomplete vascular strand formation and duplication of the longitudinal axis. Misexpression of the auxin reporter proIAA2ratioGUS and mislocalization of PIN proteins occurs in the mutants. Introduction of the ethylene-insensitive ein2 mutation partially rescues defective cell division, localization of PIN proteins, and vascular strand development.<h4>Conclusions</h4>The results support a model in which sterols are required for correct auxin and ethylene crosstalk to regulate PIN localization, auxin distribution and AtHB8 expression, necessary for correct vascular development.Margaret PullenNick ClarkFatemeh ZarinkamarJennifer ToppingKeith LindseyPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 8, p e12227 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Margaret Pullen Nick Clark Fatemeh Zarinkamar Jennifer Topping Keith Lindsey Analysis of vascular development in the hydra sterol biosynthetic mutants of Arabidopsis.
description	<h4>Background</h4>The control of vascular tissue development in plants is influenced by diverse hormonal signals, but their interactions during this process are not well understood. Wild-type sterol profiles are essential for growth, tissue patterning and signalling processes in plant development, and are required for regulated vascular patterning.<h4>Methodology/principal findings</h4>Here we investigate the roles of sterols in vascular tissue development, through an analysis of the Arabidopsis mutants hydra1 and fackel/hydra2, which are defective in the enzymes sterol isomerase and sterol C-14 reductase respectively. We show that defective vascular patterning in the shoot is associated with ectopic cell divisions. Expression of the auxin-regulated AtHB8 homeobox gene is disrupted in mutant embryos and seedlings, associated with variably incomplete vascular strand formation and duplication of the longitudinal axis. Misexpression of the auxin reporter proIAA2ratioGUS and mislocalization of PIN proteins occurs in the mutants. Introduction of the ethylene-insensitive ein2 mutation partially rescues defective cell division, localization of PIN proteins, and vascular strand development.<h4>Conclusions</h4>The results support a model in which sterols are required for correct auxin and ethylene crosstalk to regulate PIN localization, auxin distribution and AtHB8 expression, necessary for correct vascular development.
format	article
author	Margaret Pullen Nick Clark Fatemeh Zarinkamar Jennifer Topping Keith Lindsey
author_facet	Margaret Pullen Nick Clark Fatemeh Zarinkamar Jennifer Topping Keith Lindsey
author_sort	Margaret Pullen
title	Analysis of vascular development in the hydra sterol biosynthetic mutants of Arabidopsis.
title_short	Analysis of vascular development in the hydra sterol biosynthetic mutants of Arabidopsis.
title_full	Analysis of vascular development in the hydra sterol biosynthetic mutants of Arabidopsis.
title_fullStr	Analysis of vascular development in the hydra sterol biosynthetic mutants of Arabidopsis.
title_full_unstemmed	Analysis of vascular development in the hydra sterol biosynthetic mutants of Arabidopsis.
title_sort	analysis of vascular development in the hydra sterol biosynthetic mutants of arabidopsis.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/a6d870899ba54f74b238937a1465c555
work_keys_str_mv	AT margaretpullen analysisofvasculardevelopmentinthehydrasterolbiosyntheticmutantsofarabidopsis AT nickclark analysisofvasculardevelopmentinthehydrasterolbiosyntheticmutantsofarabidopsis AT fatemehzarinkamar analysisofvasculardevelopmentinthehydrasterolbiosyntheticmutantsofarabidopsis AT jennifertopping analysisofvasculardevelopmentinthehydrasterolbiosyntheticmutantsofarabidopsis AT keithlindsey analysisofvasculardevelopmentinthehydrasterolbiosyntheticmutantsofarabidopsis
_version_	1718374903485300736

Analysis of vascular development in the hydra sterol biosynthetic mutants of Arabidopsis.

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