A transcriptome analysis for 24-hour continuous sampled uterus reveals circadian regulation of the key pathways involved in eggshell formation of chicken

ABSTRACT: Circadian timing system controlled the rhythmic events, for example, ovulation and oviposition in chickens. However, how biological clock mediates eggshell formation remains obscure. Here, A 24-h mRNA transcriptome analysis was carried out in the uterus of 18 chickens with similar oviposit...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Zhichao Zhang, Xiaxia Du, Shuang Lai, Gang Shu, Qing Zhu, Yaofu Tian, Diyan Li, Yan Wang, Jiandong Yang, Yao Zhang, Xiaoling Zhao
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2022
Materias:	chicken eggshell uterus rhythm clock gene Animal culture SF1-1100
Acceso en línea:	https://doaj.org/article/a6dcb3b2b24a43fbbe11723df67db9bb
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a6dcb3b2b24a43fbbe11723df67db9bb
record_format	dspace
spelling	oai:doaj.org-article:a6dcb3b2b24a43fbbe11723df67db9bb2021-11-24T04:23:04ZA transcriptome analysis for 24-hour continuous sampled uterus reveals circadian regulation of the key pathways involved in eggshell formation of chicken0032-579110.1016/j.psj.2021.101531https://doaj.org/article/a6dcb3b2b24a43fbbe11723df67db9bb2022-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0032579121005538https://doaj.org/toc/0032-5791ABSTRACT: Circadian timing system controlled the rhythmic events, for example, ovulation and oviposition in chickens. However, how biological clock mediates eggshell formation remains obscure. Here, A 24-h mRNA transcriptome analysis was carried out in the uterus of 18 chickens with similar oviposition time points to identify the rhythmic genes and to reveal critical genes and biological pathways involved in the eggshell biomineralization. JTK_CYCLE analysis and real-time PCR revealed a total of 1,793 genes from the sequencing database with 23,513 genes (FPKM>1) were rhythmic genes regulating the rhythmic system and the expression of typical clock genes Per2, Cry1, Bmal1, Clock, Per3, and Rev-erbβ were rhythmically expressed, which suggested that endogenous clock in uterus might control the eggshell mineralization. Time of peak expression of the rhythmic genes was analyzed based on their acrophase. The main phases clustered at the periods from Zeitgeber time 0 (ZT0) to ZT4 (6:00–10:00) and from ZT10 to ZT14 (16:00-20:00). The rhythmic genes were annotated to the following Gene Ontology terms rhythmic process, lyase, ATP binding, cell membrane component. KEGG pathway enrichment analysis revealed the top 15 rhythmic genes were involved in vital biological pathways, including syndecan (1, 2, 3)-mediated signaling, post-translational regulation of adheres junction stability and disassembly, FoxO family signaling, TGF-β receptor and transport of small molecular pathways. 166 of total 1,235 genes (13.4%) were defined as rhythmic transfer factors (TFs) and they were investigated expression time distribution of cis-elements of circadian clock system D-box, E-box, B-site, and Y-Box within 24 h. Results indicated that rhythmic TFs at each phase are potential drivers of their circadian transcription activities. Compared with the control, the expression abundances of ion transport elements SCNN1G, CA2, SPP1, and ATP1B1 were significantly decreased after the interference of Bmal1 gene in synchronized uterine tubular gland cells. Clock genes changed their expression along with the eggshell formation, indicating that there is circadian clock in the uterus of chicken and it regulates the expression of eggshell formation genes.Zhichao ZhangXiaxia DuShuang LaiGang ShuQing ZhuYaofu TianDiyan LiYan WangJiandong YangYao ZhangXiaoling ZhaoElsevierarticlechickeneggshelluterusrhythmclock geneAnimal cultureSF1-1100ENPoultry Science, Vol 101, Iss 1, Pp 101531- (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	chicken eggshell uterus rhythm clock gene Animal culture SF1-1100
spellingShingle	chicken eggshell uterus rhythm clock gene Animal culture SF1-1100 Zhichao Zhang Xiaxia Du Shuang Lai Gang Shu Qing Zhu Yaofu Tian Diyan Li Yan Wang Jiandong Yang Yao Zhang Xiaoling Zhao A transcriptome analysis for 24-hour continuous sampled uterus reveals circadian regulation of the key pathways involved in eggshell formation of chicken
description	ABSTRACT: Circadian timing system controlled the rhythmic events, for example, ovulation and oviposition in chickens. However, how biological clock mediates eggshell formation remains obscure. Here, A 24-h mRNA transcriptome analysis was carried out in the uterus of 18 chickens with similar oviposition time points to identify the rhythmic genes and to reveal critical genes and biological pathways involved in the eggshell biomineralization. JTK_CYCLE analysis and real-time PCR revealed a total of 1,793 genes from the sequencing database with 23,513 genes (FPKM>1) were rhythmic genes regulating the rhythmic system and the expression of typical clock genes Per2, Cry1, Bmal1, Clock, Per3, and Rev-erbβ were rhythmically expressed, which suggested that endogenous clock in uterus might control the eggshell mineralization. Time of peak expression of the rhythmic genes was analyzed based on their acrophase. The main phases clustered at the periods from Zeitgeber time 0 (ZT0) to ZT4 (6:00–10:00) and from ZT10 to ZT14 (16:00-20:00). The rhythmic genes were annotated to the following Gene Ontology terms rhythmic process, lyase, ATP binding, cell membrane component. KEGG pathway enrichment analysis revealed the top 15 rhythmic genes were involved in vital biological pathways, including syndecan (1, 2, 3)-mediated signaling, post-translational regulation of adheres junction stability and disassembly, FoxO family signaling, TGF-β receptor and transport of small molecular pathways. 166 of total 1,235 genes (13.4%) were defined as rhythmic transfer factors (TFs) and they were investigated expression time distribution of cis-elements of circadian clock system D-box, E-box, B-site, and Y-Box within 24 h. Results indicated that rhythmic TFs at each phase are potential drivers of their circadian transcription activities. Compared with the control, the expression abundances of ion transport elements SCNN1G, CA2, SPP1, and ATP1B1 were significantly decreased after the interference of Bmal1 gene in synchronized uterine tubular gland cells. Clock genes changed their expression along with the eggshell formation, indicating that there is circadian clock in the uterus of chicken and it regulates the expression of eggshell formation genes.
format	article
author	Zhichao Zhang Xiaxia Du Shuang Lai Gang Shu Qing Zhu Yaofu Tian Diyan Li Yan Wang Jiandong Yang Yao Zhang Xiaoling Zhao
author_facet	Zhichao Zhang Xiaxia Du Shuang Lai Gang Shu Qing Zhu Yaofu Tian Diyan Li Yan Wang Jiandong Yang Yao Zhang Xiaoling Zhao
author_sort	Zhichao Zhang
title	A transcriptome analysis for 24-hour continuous sampled uterus reveals circadian regulation of the key pathways involved in eggshell formation of chicken
title_short	A transcriptome analysis for 24-hour continuous sampled uterus reveals circadian regulation of the key pathways involved in eggshell formation of chicken
title_full	A transcriptome analysis for 24-hour continuous sampled uterus reveals circadian regulation of the key pathways involved in eggshell formation of chicken
title_fullStr	A transcriptome analysis for 24-hour continuous sampled uterus reveals circadian regulation of the key pathways involved in eggshell formation of chicken
title_full_unstemmed	A transcriptome analysis for 24-hour continuous sampled uterus reveals circadian regulation of the key pathways involved in eggshell formation of chicken
title_sort	transcriptome analysis for 24-hour continuous sampled uterus reveals circadian regulation of the key pathways involved in eggshell formation of chicken
publisher	Elsevier
publishDate	2022
url	https://doaj.org/article/a6dcb3b2b24a43fbbe11723df67db9bb
work_keys_str_mv	AT zhichaozhang atranscriptomeanalysisfor24hourcontinuoussampleduterusrevealscircadianregulationofthekeypathwaysinvolvedineggshellformationofchicken AT xiaxiadu atranscriptomeanalysisfor24hourcontinuoussampleduterusrevealscircadianregulationofthekeypathwaysinvolvedineggshellformationofchicken AT shuanglai atranscriptomeanalysisfor24hourcontinuoussampleduterusrevealscircadianregulationofthekeypathwaysinvolvedineggshellformationofchicken AT gangshu atranscriptomeanalysisfor24hourcontinuoussampleduterusrevealscircadianregulationofthekeypathwaysinvolvedineggshellformationofchicken AT qingzhu atranscriptomeanalysisfor24hourcontinuoussampleduterusrevealscircadianregulationofthekeypathwaysinvolvedineggshellformationofchicken AT yaofutian atranscriptomeanalysisfor24hourcontinuoussampleduterusrevealscircadianregulationofthekeypathwaysinvolvedineggshellformationofchicken AT diyanli atranscriptomeanalysisfor24hourcontinuoussampleduterusrevealscircadianregulationofthekeypathwaysinvolvedineggshellformationofchicken AT yanwang atranscriptomeanalysisfor24hourcontinuoussampleduterusrevealscircadianregulationofthekeypathwaysinvolvedineggshellformationofchicken AT jiandongyang atranscriptomeanalysisfor24hourcontinuoussampleduterusrevealscircadianregulationofthekeypathwaysinvolvedineggshellformationofchicken AT yaozhang atranscriptomeanalysisfor24hourcontinuoussampleduterusrevealscircadianregulationofthekeypathwaysinvolvedineggshellformationofchicken AT xiaolingzhao atranscriptomeanalysisfor24hourcontinuoussampleduterusrevealscircadianregulationofthekeypathwaysinvolvedineggshellformationofchicken AT zhichaozhang transcriptomeanalysisfor24hourcontinuoussampleduterusrevealscircadianregulationofthekeypathwaysinvolvedineggshellformationofchicken AT xiaxiadu transcriptomeanalysisfor24hourcontinuoussampleduterusrevealscircadianregulationofthekeypathwaysinvolvedineggshellformationofchicken AT shuanglai transcriptomeanalysisfor24hourcontinuoussampleduterusrevealscircadianregulationofthekeypathwaysinvolvedineggshellformationofchicken AT gangshu transcriptomeanalysisfor24hourcontinuoussampleduterusrevealscircadianregulationofthekeypathwaysinvolvedineggshellformationofchicken AT qingzhu transcriptomeanalysisfor24hourcontinuoussampleduterusrevealscircadianregulationofthekeypathwaysinvolvedineggshellformationofchicken AT yaofutian transcriptomeanalysisfor24hourcontinuoussampleduterusrevealscircadianregulationofthekeypathwaysinvolvedineggshellformationofchicken AT diyanli transcriptomeanalysisfor24hourcontinuoussampleduterusrevealscircadianregulationofthekeypathwaysinvolvedineggshellformationofchicken AT yanwang transcriptomeanalysisfor24hourcontinuoussampleduterusrevealscircadianregulationofthekeypathwaysinvolvedineggshellformationofchicken AT jiandongyang transcriptomeanalysisfor24hourcontinuoussampleduterusrevealscircadianregulationofthekeypathwaysinvolvedineggshellformationofchicken AT yaozhang transcriptomeanalysisfor24hourcontinuoussampleduterusrevealscircadianregulationofthekeypathwaysinvolvedineggshellformationofchicken AT xiaolingzhao transcriptomeanalysisfor24hourcontinuoussampleduterusrevealscircadianregulationofthekeypathwaysinvolvedineggshellformationofchicken
_version_	1718416052843446272

A transcriptome analysis for 24-hour continuous sampled uterus reveals circadian regulation of the key pathways involved in eggshell formation of chicken

Ejemplares similares