Ultrasonic microbubble VEGF gene delivery improves angiogenesis of senescent endothelial progenitor cells

Abstract The therapeutic effects of ultrasonic microbubble transfection (UMT)-based vascular endothelial growth factor 165 (VEGF165) gene delivery on young and senescent endothelial progenitor cells (EPCs) were investigated. By UMT, plasmid DNA (pDNA) can be delivered into both young EPCs and senesc...

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Autores principales:	Yi-Nan Lee, Yih-Jer Wu, Hsin-I. Lee, Hsueh-Hsiao Wang, Chiung-Yin Chang, Ting-Yi Tien, Chao-Feng Lin, Cheng-Huang Su, Hung-I. Yeh
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a6debfccfd0d48b29733ab2468255380
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spelling	oai:doaj.org-article:a6debfccfd0d48b29733ab24682553802021-12-02T18:18:59ZUltrasonic microbubble VEGF gene delivery improves angiogenesis of senescent endothelial progenitor cells10.1038/s41598-021-92754-32045-2322https://doaj.org/article/a6debfccfd0d48b29733ab24682553802021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92754-3https://doaj.org/toc/2045-2322Abstract The therapeutic effects of ultrasonic microbubble transfection (UMT)-based vascular endothelial growth factor 165 (VEGF165) gene delivery on young and senescent endothelial progenitor cells (EPCs) were investigated. By UMT, plasmid DNA (pDNA) can be delivered into both young EPCs and senescent EPCs. In the UMT groups, higher pDNA-derived protein expression was found in senescent EPCs than in young EPCs. Consistent with this finding, a higher intracellular level of pDNA copy number was detected in senescent EPCs, with a peak at the 2-h time point post UMT. Ultrasonic microbubble delivery with or without VEGF improved the angiogenic properties, including the proliferation and/or migration activities, of senescent EPCs. Supernatants from young and senescent EPCs subjected to UMT-mediated VEGF transfection enhanced the proliferation and migration of human aortic endothelial cells (HAECs), and the supernatant of senescent EPCs enhanced proliferation more strongly than the supernatant from young EPCs. In the UMT groups, the stronger enhancing effect of the supernatant from senescent cells on HAEC proliferation was consistent with the higher intracellular VEGF pDNA copy number and level of protein production per cell in the supernatant from senescent cells in comparison to the supernatant from young EPCs. Given that limitations for cell therapies are the inadequate number of transplanted cells and/or insufficient cell angiogenesis, these findings provide a foundation for enhancing the therapeutic angiogenic effect of cell therapy with senescent EPCs in ischaemic cardiovascular diseases.Yi-Nan LeeYih-Jer WuHsin-I. LeeHsueh-Hsiao WangChiung-Yin ChangTing-Yi TienChao-Feng LinCheng-Huang SuHung-I. YehNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yi-Nan Lee Yih-Jer Wu Hsin-I. Lee Hsueh-Hsiao Wang Chiung-Yin Chang Ting-Yi Tien Chao-Feng Lin Cheng-Huang Su Hung-I. Yeh Ultrasonic microbubble VEGF gene delivery improves angiogenesis of senescent endothelial progenitor cells
description	Abstract The therapeutic effects of ultrasonic microbubble transfection (UMT)-based vascular endothelial growth factor 165 (VEGF165) gene delivery on young and senescent endothelial progenitor cells (EPCs) were investigated. By UMT, plasmid DNA (pDNA) can be delivered into both young EPCs and senescent EPCs. In the UMT groups, higher pDNA-derived protein expression was found in senescent EPCs than in young EPCs. Consistent with this finding, a higher intracellular level of pDNA copy number was detected in senescent EPCs, with a peak at the 2-h time point post UMT. Ultrasonic microbubble delivery with or without VEGF improved the angiogenic properties, including the proliferation and/or migration activities, of senescent EPCs. Supernatants from young and senescent EPCs subjected to UMT-mediated VEGF transfection enhanced the proliferation and migration of human aortic endothelial cells (HAECs), and the supernatant of senescent EPCs enhanced proliferation more strongly than the supernatant from young EPCs. In the UMT groups, the stronger enhancing effect of the supernatant from senescent cells on HAEC proliferation was consistent with the higher intracellular VEGF pDNA copy number and level of protein production per cell in the supernatant from senescent cells in comparison to the supernatant from young EPCs. Given that limitations for cell therapies are the inadequate number of transplanted cells and/or insufficient cell angiogenesis, these findings provide a foundation for enhancing the therapeutic angiogenic effect of cell therapy with senescent EPCs in ischaemic cardiovascular diseases.
format	article
author	Yi-Nan Lee Yih-Jer Wu Hsin-I. Lee Hsueh-Hsiao Wang Chiung-Yin Chang Ting-Yi Tien Chao-Feng Lin Cheng-Huang Su Hung-I. Yeh
author_facet	Yi-Nan Lee Yih-Jer Wu Hsin-I. Lee Hsueh-Hsiao Wang Chiung-Yin Chang Ting-Yi Tien Chao-Feng Lin Cheng-Huang Su Hung-I. Yeh
author_sort	Yi-Nan Lee
title	Ultrasonic microbubble VEGF gene delivery improves angiogenesis of senescent endothelial progenitor cells
title_short	Ultrasonic microbubble VEGF gene delivery improves angiogenesis of senescent endothelial progenitor cells
title_full	Ultrasonic microbubble VEGF gene delivery improves angiogenesis of senescent endothelial progenitor cells
title_fullStr	Ultrasonic microbubble VEGF gene delivery improves angiogenesis of senescent endothelial progenitor cells
title_full_unstemmed	Ultrasonic microbubble VEGF gene delivery improves angiogenesis of senescent endothelial progenitor cells
title_sort	ultrasonic microbubble vegf gene delivery improves angiogenesis of senescent endothelial progenitor cells
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/a6debfccfd0d48b29733ab2468255380
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Ultrasonic microbubble VEGF gene delivery improves angiogenesis of senescent endothelial progenitor cells

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