ECAT11/L1td1 is enriched in ESCs and rapidly activated during iPSC generation, but it is dispensable for the maintenance and induction of pluripotency.

The principal factors that lead to proliferation and pluripotency in embryonic stem cells (ESCs) have been vigorously investigated. However, the global network of factors and their full signaling cascade is still unclear. In this study, we found that ECAT11 (L1td1) is one of the ESC-associated trans...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Kumiko A Iwabuchi, Tatsuya Yamakawa, Yoshiko Sato, Tomoko Ichisaka, Kazutoshi Takahashi, Keisuke Okita, Shinya Yamanaka
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a6f2fc3d395d45a6b849ba409d79c6fb
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a6f2fc3d395d45a6b849ba409d79c6fb
record_format	dspace
spelling	oai:doaj.org-article:a6f2fc3d395d45a6b849ba409d79c6fb2021-11-18T06:53:10ZECAT11/L1td1 is enriched in ESCs and rapidly activated during iPSC generation, but it is dispensable for the maintenance and induction of pluripotency.1932-620310.1371/journal.pone.0020461https://doaj.org/article/a6f2fc3d395d45a6b849ba409d79c6fb2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21637830/?tool=EBIhttps://doaj.org/toc/1932-6203The principal factors that lead to proliferation and pluripotency in embryonic stem cells (ESCs) have been vigorously investigated. However, the global network of factors and their full signaling cascade is still unclear. In this study, we found that ECAT11 (L1td1) is one of the ESC-associated transcripts harboring a truncated fragment of ORF-1, a component of the L1 retrotransposable element. We generated an ECAT11 knock-in mouse by replacing its coding region with green fluorescent protein. In the early stage of development, the fluorescence was observed at the inner cell mass of blastocysts and epiblasts. Despite this specific expression, ECAT11-null mice grow normally and are fertile. In addition, ECAT11 was dispensable for both the proliferation and pluripotency of ESCs.We found rapid and robust activation of ECAT11 in fibroblasts after the forced expression of transcription factors that can give rise pluripotency in somatic cells. However, iPS cells could be established from ECAT11-null fibroblasts. Our data demonstrate the dispensability of ECAT11/L1td1 in pluripotency, despite its specific expression.Kumiko A IwabuchiTatsuya YamakawaYoshiko SatoTomoko IchisakaKazutoshi TakahashiKeisuke OkitaShinya YamanakaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 5, p e20461 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kumiko A Iwabuchi Tatsuya Yamakawa Yoshiko Sato Tomoko Ichisaka Kazutoshi Takahashi Keisuke Okita Shinya Yamanaka ECAT11/L1td1 is enriched in ESCs and rapidly activated during iPSC generation, but it is dispensable for the maintenance and induction of pluripotency.
description	The principal factors that lead to proliferation and pluripotency in embryonic stem cells (ESCs) have been vigorously investigated. However, the global network of factors and their full signaling cascade is still unclear. In this study, we found that ECAT11 (L1td1) is one of the ESC-associated transcripts harboring a truncated fragment of ORF-1, a component of the L1 retrotransposable element. We generated an ECAT11 knock-in mouse by replacing its coding region with green fluorescent protein. In the early stage of development, the fluorescence was observed at the inner cell mass of blastocysts and epiblasts. Despite this specific expression, ECAT11-null mice grow normally and are fertile. In addition, ECAT11 was dispensable for both the proliferation and pluripotency of ESCs.We found rapid and robust activation of ECAT11 in fibroblasts after the forced expression of transcription factors that can give rise pluripotency in somatic cells. However, iPS cells could be established from ECAT11-null fibroblasts. Our data demonstrate the dispensability of ECAT11/L1td1 in pluripotency, despite its specific expression.
format	article
author	Kumiko A Iwabuchi Tatsuya Yamakawa Yoshiko Sato Tomoko Ichisaka Kazutoshi Takahashi Keisuke Okita Shinya Yamanaka
author_facet	Kumiko A Iwabuchi Tatsuya Yamakawa Yoshiko Sato Tomoko Ichisaka Kazutoshi Takahashi Keisuke Okita Shinya Yamanaka
author_sort	Kumiko A Iwabuchi
title	ECAT11/L1td1 is enriched in ESCs and rapidly activated during iPSC generation, but it is dispensable for the maintenance and induction of pluripotency.
title_short	ECAT11/L1td1 is enriched in ESCs and rapidly activated during iPSC generation, but it is dispensable for the maintenance and induction of pluripotency.
title_full	ECAT11/L1td1 is enriched in ESCs and rapidly activated during iPSC generation, but it is dispensable for the maintenance and induction of pluripotency.
title_fullStr	ECAT11/L1td1 is enriched in ESCs and rapidly activated during iPSC generation, but it is dispensable for the maintenance and induction of pluripotency.
title_full_unstemmed	ECAT11/L1td1 is enriched in ESCs and rapidly activated during iPSC generation, but it is dispensable for the maintenance and induction of pluripotency.
title_sort	ecat11/l1td1 is enriched in escs and rapidly activated during ipsc generation, but it is dispensable for the maintenance and induction of pluripotency.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/a6f2fc3d395d45a6b849ba409d79c6fb
work_keys_str_mv	AT kumikoaiwabuchi ecat11l1td1isenrichedinescsandrapidlyactivatedduringipscgenerationbutitisdispensableforthemaintenanceandinductionofpluripotency AT tatsuyayamakawa ecat11l1td1isenrichedinescsandrapidlyactivatedduringipscgenerationbutitisdispensableforthemaintenanceandinductionofpluripotency AT yoshikosato ecat11l1td1isenrichedinescsandrapidlyactivatedduringipscgenerationbutitisdispensableforthemaintenanceandinductionofpluripotency AT tomokoichisaka ecat11l1td1isenrichedinescsandrapidlyactivatedduringipscgenerationbutitisdispensableforthemaintenanceandinductionofpluripotency AT kazutoshitakahashi ecat11l1td1isenrichedinescsandrapidlyactivatedduringipscgenerationbutitisdispensableforthemaintenanceandinductionofpluripotency AT keisukeokita ecat11l1td1isenrichedinescsandrapidlyactivatedduringipscgenerationbutitisdispensableforthemaintenanceandinductionofpluripotency AT shinyayamanaka ecat11l1td1isenrichedinescsandrapidlyactivatedduringipscgenerationbutitisdispensableforthemaintenanceandinductionofpluripotency
_version_	1718424246367027200

ECAT11/L1td1 is enriched in ESCs and rapidly activated during iPSC generation, but it is dispensable for the maintenance and induction of pluripotency.

Ejemplares similares