Experimental Adaptation of the Yellow Fever Virus to the Mosquito Aedes albopictus and Potential risk of urban epidemics in Brazil, South America

Abstract Despite the availability of an efficient vaccine, Yellow fever (YF), a viral disease transmitted by mosquitoes, is still a threat. In Brazil, the yellow fever virus (YFV) has been restricted to a jungle cycle for more than 70 years. However, YFV has recently invaded populated cities in the...

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Autores principales:	Fadila Amraoui, Adrien Pain, Géraldine Piorkowski, Marie Vazeille, Dinair Couto-Lima, Xavier de Lamballerie, Ricardo Lourenço-de-Oliveira, Anna-Bella Failloux
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Yellow Fever Virus (YFV) Jungle Cycle Manaus Mosquito Saliva Codon Usage Bias Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a6f8627a38394888b4b1ae1c25d87369
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spelling	oai:doaj.org-article:a6f8627a38394888b4b1ae1c25d873692021-12-02T15:09:03ZExperimental Adaptation of the Yellow Fever Virus to the Mosquito Aedes albopictus and Potential risk of urban epidemics in Brazil, South America10.1038/s41598-018-32198-42045-2322https://doaj.org/article/a6f8627a38394888b4b1ae1c25d873692018-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-32198-4https://doaj.org/toc/2045-2322Abstract Despite the availability of an efficient vaccine, Yellow fever (YF), a viral disease transmitted by mosquitoes, is still a threat. In Brazil, the yellow fever virus (YFV) has been restricted to a jungle cycle for more than 70 years. However, YFV has recently invaded populated cities in the Southeast such as Rio de Janeiro where the opportunistic mosquito Aedes albopictus is well established. Using in vivo passages of YFV in Ae. albopictus, we have selected viral strains presenting substitutions in NS1 gene. We did 10 passages of YFV-74018 on two distinct Ae. albopictus populations: (i) Manaus collected from a YFV-endemic area in Amazonia and (ii) PNMNI from a YFV-free area in the state of Rio de Janeiro. Full viral genomes were deep sequenced at each passage. We obtained two YFV strains presenting a non-synonymous substitution in the NS1 gene. Interestingly, they intervened at two different positions in NS1 gene according to the mosquito population: I2772T in Ae. albopictus Manaus and S3303N in Ae. albopictus PNMNI. Both substitutions reached fixation at the passage 10. Our data suggest that YFV has the potential for adaption to Ae. albopictus thereby posing a threat to most cities in South America where this mosquito is present.Fadila AmraouiAdrien PainGéraldine PiorkowskiMarie VazeilleDinair Couto-LimaXavier de LamballerieRicardo Lourenço-de-OliveiraAnna-Bella FaillouxNature PortfolioarticleYellow Fever Virus (YFV)Jungle CycleManausMosquito SalivaCodon Usage BiasMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-8 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Yellow Fever Virus (YFV) Jungle Cycle Manaus Mosquito Saliva Codon Usage Bias Medicine R Science Q
spellingShingle	Yellow Fever Virus (YFV) Jungle Cycle Manaus Mosquito Saliva Codon Usage Bias Medicine R Science Q Fadila Amraoui Adrien Pain Géraldine Piorkowski Marie Vazeille Dinair Couto-Lima Xavier de Lamballerie Ricardo Lourenço-de-Oliveira Anna-Bella Failloux Experimental Adaptation of the Yellow Fever Virus to the Mosquito Aedes albopictus and Potential risk of urban epidemics in Brazil, South America
description	Abstract Despite the availability of an efficient vaccine, Yellow fever (YF), a viral disease transmitted by mosquitoes, is still a threat. In Brazil, the yellow fever virus (YFV) has been restricted to a jungle cycle for more than 70 years. However, YFV has recently invaded populated cities in the Southeast such as Rio de Janeiro where the opportunistic mosquito Aedes albopictus is well established. Using in vivo passages of YFV in Ae. albopictus, we have selected viral strains presenting substitutions in NS1 gene. We did 10 passages of YFV-74018 on two distinct Ae. albopictus populations: (i) Manaus collected from a YFV-endemic area in Amazonia and (ii) PNMNI from a YFV-free area in the state of Rio de Janeiro. Full viral genomes were deep sequenced at each passage. We obtained two YFV strains presenting a non-synonymous substitution in the NS1 gene. Interestingly, they intervened at two different positions in NS1 gene according to the mosquito population: I2772T in Ae. albopictus Manaus and S3303N in Ae. albopictus PNMNI. Both substitutions reached fixation at the passage 10. Our data suggest that YFV has the potential for adaption to Ae. albopictus thereby posing a threat to most cities in South America where this mosquito is present.
format	article
author	Fadila Amraoui Adrien Pain Géraldine Piorkowski Marie Vazeille Dinair Couto-Lima Xavier de Lamballerie Ricardo Lourenço-de-Oliveira Anna-Bella Failloux
author_facet	Fadila Amraoui Adrien Pain Géraldine Piorkowski Marie Vazeille Dinair Couto-Lima Xavier de Lamballerie Ricardo Lourenço-de-Oliveira Anna-Bella Failloux
author_sort	Fadila Amraoui
title	Experimental Adaptation of the Yellow Fever Virus to the Mosquito Aedes albopictus and Potential risk of urban epidemics in Brazil, South America
title_short	Experimental Adaptation of the Yellow Fever Virus to the Mosquito Aedes albopictus and Potential risk of urban epidemics in Brazil, South America
title_full	Experimental Adaptation of the Yellow Fever Virus to the Mosquito Aedes albopictus and Potential risk of urban epidemics in Brazil, South America
title_fullStr	Experimental Adaptation of the Yellow Fever Virus to the Mosquito Aedes albopictus and Potential risk of urban epidemics in Brazil, South America
title_full_unstemmed	Experimental Adaptation of the Yellow Fever Virus to the Mosquito Aedes albopictus and Potential risk of urban epidemics in Brazil, South America
title_sort	experimental adaptation of the yellow fever virus to the mosquito aedes albopictus and potential risk of urban epidemics in brazil, south america
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/a6f8627a38394888b4b1ae1c25d87369
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Experimental Adaptation of the Yellow Fever Virus to the Mosquito Aedes albopictus and Potential risk of urban epidemics in Brazil, South America

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