IN VITRO GENERATION AND CHARACTERIZATION OF HUMAN REGULATORY T-CELLS

Abstract. The aim of present study was to evaluate efficacy of generation and characterization of regulatory T cells (Treg). The Treg were derived from peripheral mononuclear cells of healthy donors, using activation with anti-CD3mAb and IL-2, either in absence (model 1), or in the presence of immun...

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Autores principales:	E. V. Kurganova, E. Ya. Shevela, M. A. Tihonova, A. A. Ostanin, E. R. Chernykh
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2014
Materias:	тreg cd4cd25 cd4foxp3 il-10 vitamin d3 dexamethasone Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/a6ff34451673448cbc832128ac821826
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spelling	oai:doaj.org-article:a6ff34451673448cbc832128ac8218262021-11-18T08:03:38ZIN VITRO GENERATION AND CHARACTERIZATION OF HUMAN REGULATORY T-CELLS1563-06252313-741X10.15789/1563-0625-2008-2-3-173-180https://doaj.org/article/a6ff34451673448cbc832128ac8218262014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/122https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XAbstract. The aim of present study was to evaluate efficacy of generation and characterization of regulatory T cells (Treg). The Treg were derived from peripheral mononuclear cells of healthy donors, using activation with anti-CD3mAb and IL-2, either in absence (model 1), or in the presence of immunosuppressive agents (vitamin D3 and dexamethasone, model 2). It was shown, that the Treg, obtained in model 1, are characterized by decreased anti-CD3-induced proliferative response and lack of suppressive activity. Treg, obtained in presence of vitamin D3 and dexamethasone, proved to be profoundly anergic. Such a state may be partially abolished by exogenic IL-2. These cells are also characterized by suppressive activity mediated by both cell-to-cell contacts and soluble factors, such as IL-10. Hence, the regulatory T cells generated in model 2, can be referred as inducible Treg (Tr1). Nevertheless, increased ratio of cells expressing CD4+FOXP3+ among Treg obtained by model 2, may also suggest an ability to generate natural regulatory cells. (Med. Immunol., 2008, vol. 10, n 2-3, pp 173-180).E. V. KurganovaE. Ya. ShevelaM. A. TihonovaA. A. OstaninE. R. ChernykhSPb RAACIarticleтregcd4cd25cd4foxp3il-10vitamin d3dexamethasoneImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 10, Iss 2-3, Pp 173-180 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	тreg cd4cd25 cd4foxp3 il-10 vitamin d3 dexamethasone Immunologic diseases. Allergy RC581-607
spellingShingle	тreg cd4cd25 cd4foxp3 il-10 vitamin d3 dexamethasone Immunologic diseases. Allergy RC581-607 E. V. Kurganova E. Ya. Shevela M. A. Tihonova A. A. Ostanin E. R. Chernykh IN VITRO GENERATION AND CHARACTERIZATION OF HUMAN REGULATORY T-CELLS
description	Abstract. The aim of present study was to evaluate efficacy of generation and characterization of regulatory T cells (Treg). The Treg were derived from peripheral mononuclear cells of healthy donors, using activation with anti-CD3mAb and IL-2, either in absence (model 1), or in the presence of immunosuppressive agents (vitamin D3 and dexamethasone, model 2). It was shown, that the Treg, obtained in model 1, are characterized by decreased anti-CD3-induced proliferative response and lack of suppressive activity. Treg, obtained in presence of vitamin D3 and dexamethasone, proved to be profoundly anergic. Such a state may be partially abolished by exogenic IL-2. These cells are also characterized by suppressive activity mediated by both cell-to-cell contacts and soluble factors, such as IL-10. Hence, the regulatory T cells generated in model 2, can be referred as inducible Treg (Tr1). Nevertheless, increased ratio of cells expressing CD4+FOXP3+ among Treg obtained by model 2, may also suggest an ability to generate natural regulatory cells. (Med. Immunol., 2008, vol. 10, n 2-3, pp 173-180).
format	article
author	E. V. Kurganova E. Ya. Shevela M. A. Tihonova A. A. Ostanin E. R. Chernykh
author_facet	E. V. Kurganova E. Ya. Shevela M. A. Tihonova A. A. Ostanin E. R. Chernykh
author_sort	E. V. Kurganova
title	IN VITRO GENERATION AND CHARACTERIZATION OF HUMAN REGULATORY T-CELLS
title_short	IN VITRO GENERATION AND CHARACTERIZATION OF HUMAN REGULATORY T-CELLS
title_full	IN VITRO GENERATION AND CHARACTERIZATION OF HUMAN REGULATORY T-CELLS
title_fullStr	IN VITRO GENERATION AND CHARACTERIZATION OF HUMAN REGULATORY T-CELLS
title_full_unstemmed	IN VITRO GENERATION AND CHARACTERIZATION OF HUMAN REGULATORY T-CELLS
title_sort	in vitro generation and characterization of human regulatory t-cells
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/a6ff34451673448cbc832128ac821826
work_keys_str_mv	AT evkurganova invitrogenerationandcharacterizationofhumanregulatorytcells AT eyashevela invitrogenerationandcharacterizationofhumanregulatorytcells AT matihonova invitrogenerationandcharacterizationofhumanregulatorytcells AT aaostanin invitrogenerationandcharacterizationofhumanregulatorytcells AT erchernykh invitrogenerationandcharacterizationofhumanregulatorytcells
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IN VITRO GENERATION AND CHARACTERIZATION OF HUMAN REGULATORY T-CELLS

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