Multi-color live-cell super-resolution volume imaging with multi-angle interference microscopy

3D super-resolution imaging of dynamic processes in live cells is still challenging, especially in a large field of view. Here the authors combine SIM with multi-angle evanescent light illumination and achieve improved lateral and axial resolution, with stack acquisition time in the range of 1–2 s....

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Auteurs principaux: Youhua Chen, Wenjie Liu, Zhimin Zhang, Cheng Zheng, Yujia Huang, Ruizhi Cao, Dazhao Zhu, Liang Xu, Meng Zhang, Yu-Hui Zhang, Jiannan Fan, Luhong Jin, Yingke Xu, Cuifang Kuang, Xu Liu
Format: article
Langue:EN
Publié: Nature Portfolio 2018
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Accès en ligne:https://doaj.org/article/a700a165b25346efab2d752e26bb7ddf
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Résumé:3D super-resolution imaging of dynamic processes in live cells is still challenging, especially in a large field of view. Here the authors combine SIM with multi-angle evanescent light illumination and achieve improved lateral and axial resolution, with stack acquisition time in the range of 1–2 s.