Serum peptidome variations in a healthy population: reference to identify cancer-specific peptides.

The emergence of mass spectrometry (MS)-based signatures as biomarkers has generated considerable enthusiasm among oncologists. However, variations in normal individuals also exist, and a better understanding of serum peptide patterns of healthy individuals will be important for further exploring di...

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Autores principales:	Kun He, Xin-Yu Wen, Ai-Ling Li, Tao Li, Jie Wang, Hong-Xia Wang, Na Wang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a702d2c0dad940c2bb6e9af1e1f6bea8
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spelling	oai:doaj.org-article:a702d2c0dad940c2bb6e9af1e1f6bea82021-11-18T07:46:18ZSerum peptidome variations in a healthy population: reference to identify cancer-specific peptides.1932-620310.1371/journal.pone.0063724https://doaj.org/article/a702d2c0dad940c2bb6e9af1e1f6bea82013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23667664/?tool=EBIhttps://doaj.org/toc/1932-6203The emergence of mass spectrometry (MS)-based signatures as biomarkers has generated considerable enthusiasm among oncologists. However, variations in normal individuals also exist, and a better understanding of serum peptide patterns of healthy individuals will be important for further exploring disease-specific serum peptide patterns. Following development of a serum peptide pattern platform, we analyzed 500 serum samples obtained from healthy individuals. Samples from breast (n = 84), lung (n = 70), and rectal (n = 30) cancer patients were also examined. Extensive data analysis revealed negligible contributions of age to serum peptide patterns except in healthy individuals between 20-30 and 60+ years of age. Gender-related variations in the serum patterns of healthy individuals were only observed in 20-30 year-old individuals. Our results revealed substantial variation in individual peptide profiles, but 65 peptides were detected at a 20% higher frequency in the healthy population. A peptide profile was developed for each type of cancer, containing 10 discriminating peptides not prevalent in healthy individuals. Sequence identification of 111 signature peptides revealed that they fell into several tight clusters and most were exopeptidase products of serum resident proteins. We have obtained a MS-based serum peptide profile for healthy individuals, providing a reference for observing the occurrence of cancer-specific peptides.Kun HeXin-Yu WenAi-Ling LiTao LiJie WangHong-Xia WangNa WangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 5, p e63724 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kun He Xin-Yu Wen Ai-Ling Li Tao Li Jie Wang Hong-Xia Wang Na Wang Serum peptidome variations in a healthy population: reference to identify cancer-specific peptides.
description	The emergence of mass spectrometry (MS)-based signatures as biomarkers has generated considerable enthusiasm among oncologists. However, variations in normal individuals also exist, and a better understanding of serum peptide patterns of healthy individuals will be important for further exploring disease-specific serum peptide patterns. Following development of a serum peptide pattern platform, we analyzed 500 serum samples obtained from healthy individuals. Samples from breast (n = 84), lung (n = 70), and rectal (n = 30) cancer patients were also examined. Extensive data analysis revealed negligible contributions of age to serum peptide patterns except in healthy individuals between 20-30 and 60+ years of age. Gender-related variations in the serum patterns of healthy individuals were only observed in 20-30 year-old individuals. Our results revealed substantial variation in individual peptide profiles, but 65 peptides were detected at a 20% higher frequency in the healthy population. A peptide profile was developed for each type of cancer, containing 10 discriminating peptides not prevalent in healthy individuals. Sequence identification of 111 signature peptides revealed that they fell into several tight clusters and most were exopeptidase products of serum resident proteins. We have obtained a MS-based serum peptide profile for healthy individuals, providing a reference for observing the occurrence of cancer-specific peptides.
format	article
author	Kun He Xin-Yu Wen Ai-Ling Li Tao Li Jie Wang Hong-Xia Wang Na Wang
author_facet	Kun He Xin-Yu Wen Ai-Ling Li Tao Li Jie Wang Hong-Xia Wang Na Wang
author_sort	Kun He
title	Serum peptidome variations in a healthy population: reference to identify cancer-specific peptides.
title_short	Serum peptidome variations in a healthy population: reference to identify cancer-specific peptides.
title_full	Serum peptidome variations in a healthy population: reference to identify cancer-specific peptides.
title_fullStr	Serum peptidome variations in a healthy population: reference to identify cancer-specific peptides.
title_full_unstemmed	Serum peptidome variations in a healthy population: reference to identify cancer-specific peptides.
title_sort	serum peptidome variations in a healthy population: reference to identify cancer-specific peptides.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/a702d2c0dad940c2bb6e9af1e1f6bea8
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Serum peptidome variations in a healthy population: reference to identify cancer-specific peptides.

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