Effects of propofol and its formulation components on macrophages and neutrophils in obese and lean animals

Abstract We hypothesized whether propofol or active propofol component (2,6‐diisopropylphenol [DIPPH] and lipid excipient [LIP‐EXC]) separately may alter inflammatory mediators expressed by macrophages and neutrophils in lean and obese rats. Male Wistar rats (n = 10) were randomly assigned to receiv...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Luciana Boavista Barros Heil, Fernanda Ferreira Cruz, Mariana Alves Antunes, Cassia Lisboa Braga, Lais Costa Agra, Rebecca Madureira Bose Leão, Soraia Carvalho Abreu, Paolo Pelosi, Pedro Leme Silva, Patricia Rieken Macedo Rocco
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	adipose tissue inflammation lung macrophages neutrophils obesity Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/a7044bc4b9fa4625b8ff2b85bdfb27b4
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a7044bc4b9fa4625b8ff2b85bdfb27b4
record_format	dspace
spelling	oai:doaj.org-article:a7044bc4b9fa4625b8ff2b85bdfb27b42021-11-16T13:45:55ZEffects of propofol and its formulation components on macrophages and neutrophils in obese and lean animals2052-170710.1002/prp2.873https://doaj.org/article/a7044bc4b9fa4625b8ff2b85bdfb27b42021-10-01T00:00:00Zhttps://doi.org/10.1002/prp2.873https://doaj.org/toc/2052-1707Abstract We hypothesized whether propofol or active propofol component (2,6‐diisopropylphenol [DIPPH] and lipid excipient [LIP‐EXC]) separately may alter inflammatory mediators expressed by macrophages and neutrophils in lean and obese rats. Male Wistar rats (n = 10) were randomly assigned to receive a standard (lean) or obesity‐inducing diet (obese) for 12 weeks. Animals were euthanized, and alveolar macrophages and neutrophils from lean and obese animals were exposed to propofol (50 µM), active propofol component (50 µM, 2,6‐DIPPH), and lipid excipient (soybean oil, purified egg phospholipid, and glycerol) for 1 h. The primary outcome was IL‐6 expression after propofol and its components exposure by alveolar macrophages extracted from bronchoalveolar lavage fluid. The secondary outcomes were the production of mediators released by macrophages from adipose tissue, and neutrophils from lung and adipose tissues, and neutrophil migration. IL‐6 increased after the exposure to both propofol (median [interquartile range] 4.14[1.95–5.20]; p = .04) and its active component (2,6‐DIPPH) (4.09[1.67–5.91]; p = .04) in alveolar macrophages from obese animals. However, only 2,6‐DIPPH increased IL‐10 expression (7.59[6.28–12.95]; p = .001) in adipose tissue‐derived macrophages. Additionally, 2,6‐DIPPH increased C‐X‐C chemokine receptor 2 and 4 (CXCR2 and CXCR4, respectively) in lung (10.08[8.23–29.01]; p = .02; 1.55[1.49–3.43]; p = .02) and adipose tissues (8.78[4.15–11.57]; p = .03; 2.86[2.17–3.71]; p = .01), as well as improved lung‐derived neutrophil migration (28.00[−3.42 to 45.07]; p = .001). In obesity, the active component of propofol affected both the M1 and M2 markers as well as neutrophils in both alveolar and adipose tissue cells, suggesting that lipid excipient may hinder the effects of active propofol.Luciana Boavista Barros HeilFernanda Ferreira CruzMariana Alves AntunesCassia Lisboa BragaLais Costa AgraRebecca Madureira Bose LeãoSoraia Carvalho AbreuPaolo PelosiPedro Leme SilvaPatricia Rieken Macedo RoccoWileyarticleadipose tissueinflammationlungmacrophagesneutrophilsobesityTherapeutics. PharmacologyRM1-950ENPharmacology Research & Perspectives, Vol 9, Iss 5, Pp n/a-n/a (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	adipose tissue inflammation lung macrophages neutrophils obesity Therapeutics. Pharmacology RM1-950
spellingShingle	adipose tissue inflammation lung macrophages neutrophils obesity Therapeutics. Pharmacology RM1-950 Luciana Boavista Barros Heil Fernanda Ferreira Cruz Mariana Alves Antunes Cassia Lisboa Braga Lais Costa Agra Rebecca Madureira Bose Leão Soraia Carvalho Abreu Paolo Pelosi Pedro Leme Silva Patricia Rieken Macedo Rocco Effects of propofol and its formulation components on macrophages and neutrophils in obese and lean animals
description	Abstract We hypothesized whether propofol or active propofol component (2,6‐diisopropylphenol [DIPPH] and lipid excipient [LIP‐EXC]) separately may alter inflammatory mediators expressed by macrophages and neutrophils in lean and obese rats. Male Wistar rats (n = 10) were randomly assigned to receive a standard (lean) or obesity‐inducing diet (obese) for 12 weeks. Animals were euthanized, and alveolar macrophages and neutrophils from lean and obese animals were exposed to propofol (50 µM), active propofol component (50 µM, 2,6‐DIPPH), and lipid excipient (soybean oil, purified egg phospholipid, and glycerol) for 1 h. The primary outcome was IL‐6 expression after propofol and its components exposure by alveolar macrophages extracted from bronchoalveolar lavage fluid. The secondary outcomes were the production of mediators released by macrophages from adipose tissue, and neutrophils from lung and adipose tissues, and neutrophil migration. IL‐6 increased after the exposure to both propofol (median [interquartile range] 4.14[1.95–5.20]; p = .04) and its active component (2,6‐DIPPH) (4.09[1.67–5.91]; p = .04) in alveolar macrophages from obese animals. However, only 2,6‐DIPPH increased IL‐10 expression (7.59[6.28–12.95]; p = .001) in adipose tissue‐derived macrophages. Additionally, 2,6‐DIPPH increased C‐X‐C chemokine receptor 2 and 4 (CXCR2 and CXCR4, respectively) in lung (10.08[8.23–29.01]; p = .02; 1.55[1.49–3.43]; p = .02) and adipose tissues (8.78[4.15–11.57]; p = .03; 2.86[2.17–3.71]; p = .01), as well as improved lung‐derived neutrophil migration (28.00[−3.42 to 45.07]; p = .001). In obesity, the active component of propofol affected both the M1 and M2 markers as well as neutrophils in both alveolar and adipose tissue cells, suggesting that lipid excipient may hinder the effects of active propofol.
format	article
author	Luciana Boavista Barros Heil Fernanda Ferreira Cruz Mariana Alves Antunes Cassia Lisboa Braga Lais Costa Agra Rebecca Madureira Bose Leão Soraia Carvalho Abreu Paolo Pelosi Pedro Leme Silva Patricia Rieken Macedo Rocco
author_facet	Luciana Boavista Barros Heil Fernanda Ferreira Cruz Mariana Alves Antunes Cassia Lisboa Braga Lais Costa Agra Rebecca Madureira Bose Leão Soraia Carvalho Abreu Paolo Pelosi Pedro Leme Silva Patricia Rieken Macedo Rocco
author_sort	Luciana Boavista Barros Heil
title	Effects of propofol and its formulation components on macrophages and neutrophils in obese and lean animals
title_short	Effects of propofol and its formulation components on macrophages and neutrophils in obese and lean animals
title_full	Effects of propofol and its formulation components on macrophages and neutrophils in obese and lean animals
title_fullStr	Effects of propofol and its formulation components on macrophages and neutrophils in obese and lean animals
title_full_unstemmed	Effects of propofol and its formulation components on macrophages and neutrophils in obese and lean animals
title_sort	effects of propofol and its formulation components on macrophages and neutrophils in obese and lean animals
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/a7044bc4b9fa4625b8ff2b85bdfb27b4
work_keys_str_mv	AT lucianaboavistabarrosheil effectsofpropofolanditsformulationcomponentsonmacrophagesandneutrophilsinobeseandleananimals AT fernandaferreiracruz effectsofpropofolanditsformulationcomponentsonmacrophagesandneutrophilsinobeseandleananimals AT marianaalvesantunes effectsofpropofolanditsformulationcomponentsonmacrophagesandneutrophilsinobeseandleananimals AT cassialisboabraga effectsofpropofolanditsformulationcomponentsonmacrophagesandneutrophilsinobeseandleananimals AT laiscostaagra effectsofpropofolanditsformulationcomponentsonmacrophagesandneutrophilsinobeseandleananimals AT rebeccamadureiraboseleao effectsofpropofolanditsformulationcomponentsonmacrophagesandneutrophilsinobeseandleananimals AT soraiacarvalhoabreu effectsofpropofolanditsformulationcomponentsonmacrophagesandneutrophilsinobeseandleananimals AT paolopelosi effectsofpropofolanditsformulationcomponentsonmacrophagesandneutrophilsinobeseandleananimals AT pedrolemesilva effectsofpropofolanditsformulationcomponentsonmacrophagesandneutrophilsinobeseandleananimals AT patriciariekenmacedorocco effectsofpropofolanditsformulationcomponentsonmacrophagesandneutrophilsinobeseandleananimals
_version_	1718426497260191744

Effects of propofol and its formulation components on macrophages and neutrophils in obese and lean animals

Ejemplares similares