A model system for in vitro studies of bank vole borne viruses.

The bank vole (Myodes glareolus) is a common small mammal in Europe and a natural host for several important emerging zoonotic viruses, e.g. Puumala hantavirus (PUUV) that causes hemorrhagic fever with renal syndrome (HFRS). Hantaviruses are known to interfere with several signaling pathways in infe...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Malin Stoltz, Karin B Sundström, Åsa Hidmark, Conny Tolf, Sirkka Vene, Clas Ahlm, A Michael Lindberg, Åke Lundkvist, Jonas Klingström
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a70765b076114cadb55fb75bf6d92cfd
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a70765b076114cadb55fb75bf6d92cfd
record_format	dspace
spelling	oai:doaj.org-article:a70765b076114cadb55fb75bf6d92cfd2021-11-18T07:31:56ZA model system for in vitro studies of bank vole borne viruses.1932-620310.1371/journal.pone.0028992https://doaj.org/article/a70765b076114cadb55fb75bf6d92cfd2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22194969/?tool=EBIhttps://doaj.org/toc/1932-6203The bank vole (Myodes glareolus) is a common small mammal in Europe and a natural host for several important emerging zoonotic viruses, e.g. Puumala hantavirus (PUUV) that causes hemorrhagic fever with renal syndrome (HFRS). Hantaviruses are known to interfere with several signaling pathways in infected human cells, and HFRS is considered an immune-mediated disease. There is no in vitro-model available for infectious experiments in bank vole cells, nor tools for analyses of bank vole immune activation and responses. Consequently, it is not known if there are any differences in the regulation of virus induced responses in humans compared to natural hosts during infection. We here present an in vitro-model for studies of bank vole borne viruses and their interactions with natural host cell innate immune responses. Bank vole embryonic fibroblasts (VEFs) were isolated and shown to be susceptible for PUUV-infection, including a wild-type PUUV strain (only passaged in bank voles). The significance of VEFs as a model system for bank vole associated viruses was further established by infection studies showing that these cells are also susceptible to tick borne encephalitis, cowpox and Ljungan virus. The genes encoding bank vole IFN-β and Mx2 were partially sequenced and protocols for semi-quantitative RT-PCR were developed. Interestingly, PUUV did not induce an increased IFN-β or Mx2 mRNA expression. Corresponding infections with CPXV and LV induced IFN-β but not Mx2, while TBEV induced both IFN-β and Mx2. In conclusion, VEFs together with protocols developed for detection of bank vole innate immune activation provide valuable tools for future studies of how PUUV and other zoonotic viruses affect cells derived from bank voles compared to human cells. Notably, wild-type PUUV which has been difficult to cultivate in vitro readily infected VEFs, suggesting that embryonic fibroblasts from natural hosts might be valuable for isolation of wild-type hantaviruses.Malin StoltzKarin B SundströmÅsa HidmarkConny TolfSirkka VeneClas AhlmA Michael LindbergÅke LundkvistJonas KlingströmPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 12, p e28992 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Malin Stoltz Karin B Sundström Åsa Hidmark Conny Tolf Sirkka Vene Clas Ahlm A Michael Lindberg Åke Lundkvist Jonas Klingström A model system for in vitro studies of bank vole borne viruses.
description	The bank vole (Myodes glareolus) is a common small mammal in Europe and a natural host for several important emerging zoonotic viruses, e.g. Puumala hantavirus (PUUV) that causes hemorrhagic fever with renal syndrome (HFRS). Hantaviruses are known to interfere with several signaling pathways in infected human cells, and HFRS is considered an immune-mediated disease. There is no in vitro-model available for infectious experiments in bank vole cells, nor tools for analyses of bank vole immune activation and responses. Consequently, it is not known if there are any differences in the regulation of virus induced responses in humans compared to natural hosts during infection. We here present an in vitro-model for studies of bank vole borne viruses and their interactions with natural host cell innate immune responses. Bank vole embryonic fibroblasts (VEFs) were isolated and shown to be susceptible for PUUV-infection, including a wild-type PUUV strain (only passaged in bank voles). The significance of VEFs as a model system for bank vole associated viruses was further established by infection studies showing that these cells are also susceptible to tick borne encephalitis, cowpox and Ljungan virus. The genes encoding bank vole IFN-β and Mx2 were partially sequenced and protocols for semi-quantitative RT-PCR were developed. Interestingly, PUUV did not induce an increased IFN-β or Mx2 mRNA expression. Corresponding infections with CPXV and LV induced IFN-β but not Mx2, while TBEV induced both IFN-β and Mx2. In conclusion, VEFs together with protocols developed for detection of bank vole innate immune activation provide valuable tools for future studies of how PUUV and other zoonotic viruses affect cells derived from bank voles compared to human cells. Notably, wild-type PUUV which has been difficult to cultivate in vitro readily infected VEFs, suggesting that embryonic fibroblasts from natural hosts might be valuable for isolation of wild-type hantaviruses.
format	article
author	Malin Stoltz Karin B Sundström Åsa Hidmark Conny Tolf Sirkka Vene Clas Ahlm A Michael Lindberg Åke Lundkvist Jonas Klingström
author_facet	Malin Stoltz Karin B Sundström Åsa Hidmark Conny Tolf Sirkka Vene Clas Ahlm A Michael Lindberg Åke Lundkvist Jonas Klingström
author_sort	Malin Stoltz
title	A model system for in vitro studies of bank vole borne viruses.
title_short	A model system for in vitro studies of bank vole borne viruses.
title_full	A model system for in vitro studies of bank vole borne viruses.
title_fullStr	A model system for in vitro studies of bank vole borne viruses.
title_full_unstemmed	A model system for in vitro studies of bank vole borne viruses.
title_sort	model system for in vitro studies of bank vole borne viruses.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/a70765b076114cadb55fb75bf6d92cfd
work_keys_str_mv	AT malinstoltz amodelsystemforinvitrostudiesofbankvoleborneviruses AT karinbsundstrom amodelsystemforinvitrostudiesofbankvoleborneviruses AT asahidmark amodelsystemforinvitrostudiesofbankvoleborneviruses AT connytolf amodelsystemforinvitrostudiesofbankvoleborneviruses AT sirkkavene amodelsystemforinvitrostudiesofbankvoleborneviruses AT clasahlm amodelsystemforinvitrostudiesofbankvoleborneviruses AT amichaellindberg amodelsystemforinvitrostudiesofbankvoleborneviruses AT akelundkvist amodelsystemforinvitrostudiesofbankvoleborneviruses AT jonasklingstrom amodelsystemforinvitrostudiesofbankvoleborneviruses AT malinstoltz modelsystemforinvitrostudiesofbankvoleborneviruses AT karinbsundstrom modelsystemforinvitrostudiesofbankvoleborneviruses AT asahidmark modelsystemforinvitrostudiesofbankvoleborneviruses AT connytolf modelsystemforinvitrostudiesofbankvoleborneviruses AT sirkkavene modelsystemforinvitrostudiesofbankvoleborneviruses AT clasahlm modelsystemforinvitrostudiesofbankvoleborneviruses AT amichaellindberg modelsystemforinvitrostudiesofbankvoleborneviruses AT akelundkvist modelsystemforinvitrostudiesofbankvoleborneviruses AT jonasklingstrom modelsystemforinvitrostudiesofbankvoleborneviruses
_version_	1718423306023993344

A model system for in vitro studies of bank vole borne viruses.

Ejemplares similares