The utility of micro-computed tomography for the non-destructive study of eye microstructure in snails

Abstract Molluscan eyes exhibit an enormous range of morphological variation, ranging from tiny pigment-cup eyes in limpets, compound eyes in ark clams and pinhole eyes in Nautilus, through to concave mirror eyes in scallops and the large camera-type eyes of the more derived cephalopods. Here we ass...

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Autores principales:	Lauren Sumner-Rooney, Nathan J. Kenny, Farah Ahmed, Suzanne T. Williams
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a70b5c1403dd4794bea035430a25f152
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spelling	oai:doaj.org-article:a70b5c1403dd4794bea035430a25f1522021-12-02T15:09:49ZThe utility of micro-computed tomography for the non-destructive study of eye microstructure in snails10.1038/s41598-019-51909-z2045-2322https://doaj.org/article/a70b5c1403dd4794bea035430a25f1522019-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-51909-zhttps://doaj.org/toc/2045-2322Abstract Molluscan eyes exhibit an enormous range of morphological variation, ranging from tiny pigment-cup eyes in limpets, compound eyes in ark clams and pinhole eyes in Nautilus, through to concave mirror eyes in scallops and the large camera-type eyes of the more derived cephalopods. Here we assess the potential of non-destructive micro-computed tomography (µ-CT) for investigating the anatomy of molluscan eyes in three species of the family Solariellidae, a group of small, deep-sea gastropods. We compare our results directly with those from traditional histological methods applied to the same specimens, and show not only that eye microstructure can be visualised in sufficient detail for meaningful comparison even in very small animals, but also that μ-CT can provide additional insight into gross neuroanatomy without damaging rare and precious specimens. Data from μ-CT scans also show that neurological innervation of eyes is reduced in dark-adapted snails when compared with the innervation of cephalic tentacles, which are involved in mechanoreception and possibly chemoreception. Molecular tests also show that the use of µ-CT and phosphotungstic acid stain do not prevent successful downstream DNA extraction, PCR amplification or sequencing. The use of µ-CT methods is therefore highly recommended for the investigation of difficult-to-collect or unique specimens.Lauren Sumner-RooneyNathan J. KennyFarah AhmedSuzanne T. WilliamsNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-9 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lauren Sumner-Rooney Nathan J. Kenny Farah Ahmed Suzanne T. Williams The utility of micro-computed tomography for the non-destructive study of eye microstructure in snails
description	Abstract Molluscan eyes exhibit an enormous range of morphological variation, ranging from tiny pigment-cup eyes in limpets, compound eyes in ark clams and pinhole eyes in Nautilus, through to concave mirror eyes in scallops and the large camera-type eyes of the more derived cephalopods. Here we assess the potential of non-destructive micro-computed tomography (µ-CT) for investigating the anatomy of molluscan eyes in three species of the family Solariellidae, a group of small, deep-sea gastropods. We compare our results directly with those from traditional histological methods applied to the same specimens, and show not only that eye microstructure can be visualised in sufficient detail for meaningful comparison even in very small animals, but also that μ-CT can provide additional insight into gross neuroanatomy without damaging rare and precious specimens. Data from μ-CT scans also show that neurological innervation of eyes is reduced in dark-adapted snails when compared with the innervation of cephalic tentacles, which are involved in mechanoreception and possibly chemoreception. Molecular tests also show that the use of µ-CT and phosphotungstic acid stain do not prevent successful downstream DNA extraction, PCR amplification or sequencing. The use of µ-CT methods is therefore highly recommended for the investigation of difficult-to-collect or unique specimens.
format	article
author	Lauren Sumner-Rooney Nathan J. Kenny Farah Ahmed Suzanne T. Williams
author_facet	Lauren Sumner-Rooney Nathan J. Kenny Farah Ahmed Suzanne T. Williams
author_sort	Lauren Sumner-Rooney
title	The utility of micro-computed tomography for the non-destructive study of eye microstructure in snails
title_short	The utility of micro-computed tomography for the non-destructive study of eye microstructure in snails
title_full	The utility of micro-computed tomography for the non-destructive study of eye microstructure in snails
title_fullStr	The utility of micro-computed tomography for the non-destructive study of eye microstructure in snails
title_full_unstemmed	The utility of micro-computed tomography for the non-destructive study of eye microstructure in snails
title_sort	utility of micro-computed tomography for the non-destructive study of eye microstructure in snails
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/a70b5c1403dd4794bea035430a25f152
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The utility of micro-computed tomography for the non-destructive study of eye microstructure in snails

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