Structural Analysis of the Caenorhabditis elegans Dauer Larval Anterior Sensilla by Focused Ion Beam-Scanning Electron Microscopy

At the end of the first larval stage, the nematode Caenorhabditis elegans developing in harsh environmental conditions is able to choose an alternative developmental path called the dauer diapause. Dauer larvae exhibit different physiology and behaviors from non-dauer larvae. Using focused ion beam-...

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Autores principales:	Sebastian Britz, Sebastian Matthias Markert, Daniel Witvliet, Anna Maria Steyer, Sarah Tröger, Ben Mulcahy, Philip Kollmannsberger, Yannick Schwab, Mei Zhen, Christian Stigloher
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	FIB-SEM 3D reconstruction neuroanatomy IL2 branching amphids Caenorhabditis elegans (C. elegans) Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Human anatomy QM1-695
Acceso en línea:	https://doaj.org/article/a70fe88442874f8daf0bd38af6121fae
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spelling	oai:doaj.org-article:a70fe88442874f8daf0bd38af6121fae2021-11-08T08:54:56ZStructural Analysis of the Caenorhabditis elegans Dauer Larval Anterior Sensilla by Focused Ion Beam-Scanning Electron Microscopy1662-512910.3389/fnana.2021.732520https://doaj.org/article/a70fe88442874f8daf0bd38af6121fae2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fnana.2021.732520/fullhttps://doaj.org/toc/1662-5129At the end of the first larval stage, the nematode Caenorhabditis elegans developing in harsh environmental conditions is able to choose an alternative developmental path called the dauer diapause. Dauer larvae exhibit different physiology and behaviors from non-dauer larvae. Using focused ion beam-scanning electron microscopy (FIB-SEM), we volumetrically reconstructed the anterior sensory apparatus of C. elegans dauer larvae with unprecedented precision. We provide a detailed description of some neurons, focusing on structural details that were unknown or unresolved by previously published studies. They include the following: (1) dauer-specific branches of the IL2 sensory neurons project into the periphery of anterior sensilla and motor or putative sensory neurons at the sub-lateral cords; (2) ciliated endings of URX sensory neurons are supported by both ILso and AMso socket cells near the amphid openings; (3) variability in amphid sensory dendrites among dauers; and (4) somatic RIP interneurons maintain their projection into the pharyngeal nervous system. Our results support the notion that dauer larvae structurally expand their sensory system to facilitate searching for more favorable environments.Sebastian BritzSebastian Matthias MarkertDaniel WitvlietDaniel WitvlietAnna Maria SteyerSarah TrögerBen MulcahyBen MulcahyPhilip KollmannsbergerYannick SchwabMei ZhenMei ZhenChristian StigloherFrontiers Media S.A.articleFIB-SEM3D reconstructionneuroanatomyIL2 branchingamphidsCaenorhabditis elegans (C. elegans)Neurosciences. Biological psychiatry. NeuropsychiatryRC321-571Human anatomyQM1-695ENFrontiers in Neuroanatomy, Vol 15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	FIB-SEM 3D reconstruction neuroanatomy IL2 branching amphids Caenorhabditis elegans (C. elegans) Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Human anatomy QM1-695
spellingShingle	FIB-SEM 3D reconstruction neuroanatomy IL2 branching amphids Caenorhabditis elegans (C. elegans) Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Human anatomy QM1-695 Sebastian Britz Sebastian Matthias Markert Daniel Witvliet Daniel Witvliet Anna Maria Steyer Sarah Tröger Ben Mulcahy Ben Mulcahy Philip Kollmannsberger Yannick Schwab Mei Zhen Mei Zhen Christian Stigloher Structural Analysis of the Caenorhabditis elegans Dauer Larval Anterior Sensilla by Focused Ion Beam-Scanning Electron Microscopy
description	At the end of the first larval stage, the nematode Caenorhabditis elegans developing in harsh environmental conditions is able to choose an alternative developmental path called the dauer diapause. Dauer larvae exhibit different physiology and behaviors from non-dauer larvae. Using focused ion beam-scanning electron microscopy (FIB-SEM), we volumetrically reconstructed the anterior sensory apparatus of C. elegans dauer larvae with unprecedented precision. We provide a detailed description of some neurons, focusing on structural details that were unknown or unresolved by previously published studies. They include the following: (1) dauer-specific branches of the IL2 sensory neurons project into the periphery of anterior sensilla and motor or putative sensory neurons at the sub-lateral cords; (2) ciliated endings of URX sensory neurons are supported by both ILso and AMso socket cells near the amphid openings; (3) variability in amphid sensory dendrites among dauers; and (4) somatic RIP interneurons maintain their projection into the pharyngeal nervous system. Our results support the notion that dauer larvae structurally expand their sensory system to facilitate searching for more favorable environments.
format	article
author	Sebastian Britz Sebastian Matthias Markert Daniel Witvliet Daniel Witvliet Anna Maria Steyer Sarah Tröger Ben Mulcahy Ben Mulcahy Philip Kollmannsberger Yannick Schwab Mei Zhen Mei Zhen Christian Stigloher
author_facet	Sebastian Britz Sebastian Matthias Markert Daniel Witvliet Daniel Witvliet Anna Maria Steyer Sarah Tröger Ben Mulcahy Ben Mulcahy Philip Kollmannsberger Yannick Schwab Mei Zhen Mei Zhen Christian Stigloher
author_sort	Sebastian Britz
title	Structural Analysis of the Caenorhabditis elegans Dauer Larval Anterior Sensilla by Focused Ion Beam-Scanning Electron Microscopy
title_short	Structural Analysis of the Caenorhabditis elegans Dauer Larval Anterior Sensilla by Focused Ion Beam-Scanning Electron Microscopy
title_full	Structural Analysis of the Caenorhabditis elegans Dauer Larval Anterior Sensilla by Focused Ion Beam-Scanning Electron Microscopy
title_fullStr	Structural Analysis of the Caenorhabditis elegans Dauer Larval Anterior Sensilla by Focused Ion Beam-Scanning Electron Microscopy
title_full_unstemmed	Structural Analysis of the Caenorhabditis elegans Dauer Larval Anterior Sensilla by Focused Ion Beam-Scanning Electron Microscopy
title_sort	structural analysis of the caenorhabditis elegans dauer larval anterior sensilla by focused ion beam-scanning electron microscopy
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/a70fe88442874f8daf0bd38af6121fae
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Structural Analysis of the Caenorhabditis elegans Dauer Larval Anterior Sensilla by Focused Ion Beam-Scanning Electron Microscopy

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