Longitudinal evaluation of azithromycin and cytokine concentrations in amniotic fluid following one‐time oral dosing in pregnancy

Abstract To utilize noninvasive collection of amniotic fluid in the setting of preterm premature rupture of membranes (PPROMs) to report the time concentration profile of azithromycin in amniotic fluid over 7 days from a single dose, and evaluate the correlation between azithromycin concentration an...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Rupsa C. Boelig, Edwin Lam, Ankit Rochani, Gagan Kaushal, Amanda Roman, Walter K. Kraft
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	Therapeutics. Pharmacology RM1-950 Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/a71008a06db44a15a5f2fef1738f16d5
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a71008a06db44a15a5f2fef1738f16d5
record_format	dspace
spelling	oai:doaj.org-article:a71008a06db44a15a5f2fef1738f16d52021-11-19T17:51:35ZLongitudinal evaluation of azithromycin and cytokine concentrations in amniotic fluid following one‐time oral dosing in pregnancy1752-80621752-805410.1111/cts.13111https://doaj.org/article/a71008a06db44a15a5f2fef1738f16d52021-11-01T00:00:00Zhttps://doi.org/10.1111/cts.13111https://doaj.org/toc/1752-8054https://doaj.org/toc/1752-8062Abstract To utilize noninvasive collection of amniotic fluid in the setting of preterm premature rupture of membranes (PPROMs) to report the time concentration profile of azithromycin in amniotic fluid over 7 days from a single dose, and evaluate the correlation between azithromycin concentration and inflammatory markers in amniotic fluid. Prospective cohort study of five pregnant patients admitted with PPROMs and treated with a single 1 g oral azithromycin dose. Amniotic fluid was collected from pads and used to quantify azithromycin concentration as well as TNFa, IL‐1a, IL‐1b, IL‐6, IL‐8, and IL‐10 concentrations. Primary outcome was time/concentration profile of azithromycin in amniotic fluid. Secondary outcome included correlation between azithromycin concentration and cytokine concentrations. Five patients were enrolled. Mean gestational age on admission with PPROM was 27.5 ± 2.3 weeks with a median latency of 7 days (interquartile range [IQR] = 4–13). A median of two samples/day (IQR = 1–3) were collected per participant. Azithromycin was quantified in duplicate; intra‐assay coefficient of variation was 17%. Azithromycin concentration was less than 60 ng/ml after day 3. Azithromycin concentration was positively correlated with IL‐8 (r = 0.38, p = 0.03), IL1a (r = 0.39, p = 0.03), and IL‐1b (r = 0.36, p = 0.04) in amniotic fluid. Azithromycin is detectable in amniotic fluid over 7 days from a single 1 g maternal dose, however, it is not sustained over the range of minimum inhibitory concentration for common genitourinary flora. Based on correlation with specific cytokines, azithromycin penetration in amniotic fluid may relate to maternal monocyte concentration in amniotic fluid in the setting of PPROM.Rupsa C. BoeligEdwin LamAnkit RochaniGagan KaushalAmanda RomanWalter K. KraftWileyarticleTherapeutics. PharmacologyRM1-950Public aspects of medicineRA1-1270ENClinical and Translational Science, Vol 14, Iss 6, Pp 2431-2439 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Therapeutics. Pharmacology RM1-950 Public aspects of medicine RA1-1270
spellingShingle	Therapeutics. Pharmacology RM1-950 Public aspects of medicine RA1-1270 Rupsa C. Boelig Edwin Lam Ankit Rochani Gagan Kaushal Amanda Roman Walter K. Kraft Longitudinal evaluation of azithromycin and cytokine concentrations in amniotic fluid following one‐time oral dosing in pregnancy
description	Abstract To utilize noninvasive collection of amniotic fluid in the setting of preterm premature rupture of membranes (PPROMs) to report the time concentration profile of azithromycin in amniotic fluid over 7 days from a single dose, and evaluate the correlation between azithromycin concentration and inflammatory markers in amniotic fluid. Prospective cohort study of five pregnant patients admitted with PPROMs and treated with a single 1 g oral azithromycin dose. Amniotic fluid was collected from pads and used to quantify azithromycin concentration as well as TNFa, IL‐1a, IL‐1b, IL‐6, IL‐8, and IL‐10 concentrations. Primary outcome was time/concentration profile of azithromycin in amniotic fluid. Secondary outcome included correlation between azithromycin concentration and cytokine concentrations. Five patients were enrolled. Mean gestational age on admission with PPROM was 27.5 ± 2.3 weeks with a median latency of 7 days (interquartile range [IQR] = 4–13). A median of two samples/day (IQR = 1–3) were collected per participant. Azithromycin was quantified in duplicate; intra‐assay coefficient of variation was 17%. Azithromycin concentration was less than 60 ng/ml after day 3. Azithromycin concentration was positively correlated with IL‐8 (r = 0.38, p = 0.03), IL1a (r = 0.39, p = 0.03), and IL‐1b (r = 0.36, p = 0.04) in amniotic fluid. Azithromycin is detectable in amniotic fluid over 7 days from a single 1 g maternal dose, however, it is not sustained over the range of minimum inhibitory concentration for common genitourinary flora. Based on correlation with specific cytokines, azithromycin penetration in amniotic fluid may relate to maternal monocyte concentration in amniotic fluid in the setting of PPROM.
format	article
author	Rupsa C. Boelig Edwin Lam Ankit Rochani Gagan Kaushal Amanda Roman Walter K. Kraft
author_facet	Rupsa C. Boelig Edwin Lam Ankit Rochani Gagan Kaushal Amanda Roman Walter K. Kraft
author_sort	Rupsa C. Boelig
title	Longitudinal evaluation of azithromycin and cytokine concentrations in amniotic fluid following one‐time oral dosing in pregnancy
title_short	Longitudinal evaluation of azithromycin and cytokine concentrations in amniotic fluid following one‐time oral dosing in pregnancy
title_full	Longitudinal evaluation of azithromycin and cytokine concentrations in amniotic fluid following one‐time oral dosing in pregnancy
title_fullStr	Longitudinal evaluation of azithromycin and cytokine concentrations in amniotic fluid following one‐time oral dosing in pregnancy
title_full_unstemmed	Longitudinal evaluation of azithromycin and cytokine concentrations in amniotic fluid following one‐time oral dosing in pregnancy
title_sort	longitudinal evaluation of azithromycin and cytokine concentrations in amniotic fluid following one‐time oral dosing in pregnancy
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/a71008a06db44a15a5f2fef1738f16d5
work_keys_str_mv	AT rupsacboelig longitudinalevaluationofazithromycinandcytokineconcentrationsinamnioticfluidfollowingonetimeoraldosinginpregnancy AT edwinlam longitudinalevaluationofazithromycinandcytokineconcentrationsinamnioticfluidfollowingonetimeoraldosinginpregnancy AT ankitrochani longitudinalevaluationofazithromycinandcytokineconcentrationsinamnioticfluidfollowingonetimeoraldosinginpregnancy AT gagankaushal longitudinalevaluationofazithromycinandcytokineconcentrationsinamnioticfluidfollowingonetimeoraldosinginpregnancy AT amandaroman longitudinalevaluationofazithromycinandcytokineconcentrationsinamnioticfluidfollowingonetimeoraldosinginpregnancy AT walterkkraft longitudinalevaluationofazithromycinandcytokineconcentrationsinamnioticfluidfollowingonetimeoraldosinginpregnancy
_version_	1718420032340361216

Longitudinal evaluation of azithromycin and cytokine concentrations in amniotic fluid following one‐time oral dosing in pregnancy

Ejemplares similares