Curcumin improves prostanoid ratio in diabetic mesenteric arteries associated with cyclooxygenase-2 and NF-κB suppression
Sirada Rungseesantivanon1, Naris Thengchaisri4, Preecha Ruangvejvorachai2, Suthiluk Patumraj31Interdepartment of Physiology, Graduate School, 2Department of Pathology, 3Department of Physiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand; 4Department of Companion Animal Clinica...
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Dove Medical Press
2010
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oai:doaj.org-article:a718b8a1bcb04a7a82f517229c85302b2021-12-02T07:45:50ZCurcumin improves prostanoid ratio in diabetic mesenteric arteries associated with cyclooxygenase-2 and NF-κB suppression1178-7007https://doaj.org/article/a718b8a1bcb04a7a82f517229c85302b2010-12-01T00:00:00Zhttp://www.dovepress.com/curcumin-improves-prostanoid-ratio-in-diabetic-mesenteric-arteries-ass-a5819https://doaj.org/toc/1178-7007Sirada Rungseesantivanon1, Naris Thengchaisri4, Preecha Ruangvejvorachai2, Suthiluk Patumraj31Interdepartment of Physiology, Graduate School, 2Department of Pathology, 3Department of Physiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand; 4Department of Companion Animal Clinical Sciences, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, ThailandBackground: Curcumin, the active ingredient from turmeric rhizomes, has been shown to have a wide range of pharmacological properties including antioxidant and anti-inflammatory effects. Curcumin has been reviewed for its multiple molecular action on inhibiting tumor angiogenesis via its mechanisms of cyclooxygenase (COX)-2, and vascular endothelial growth factor (VEGF) inhibition. In this present study, we aimed to assess the effects of curcumin on preventing diabetes-induced vascular dysfunction in association with COX-2, nuclear factor-κB (NF-κB) expression, and prostanoid production.Methods: Twelve-week-old male Wistar rats were separated into five groups: 1) diabetes with 0.9% normal saline (DM-NSS; n = 10), 2) diabetes treated with curcumin 30 mg/kg (n = 10), 3) diabetes treated with curcumin 300 mg/kg (n = 10), 4) the control with 0.9% normal saline (n = 10), and 5) the control treated with 300 mg/kg (n = 10). Daily oral feeding of curcumin was started at 6 weeks after the streptozotocin injection. Levels of 6-keto prostaglandin (PG) F1α and thromboxane (TX) B2 were determined from mesenteric perfusates using enzyme immunoassay kits. Protein kinase C (PKC)-ßII and COX-2 with NF-κB levels were analyzed in the mesenteric arteries by immunofluorescent staining and immunohistochemistry, respectively.Results: The ratio of 6-keto-PGF1α and TXB2 was significantly decreased in DM-NSS compared with the control (P < 0.05). Double-immunofluorescent staining with specific antibodies for PKC-βII and a-smooth muscle actins showed that the diabetic mesenteric arteries contained increased of PKC-βII within the vascular wall. Also, COX-2 expression and activated NF-κB in the small mesenteric artery of diabetes mellitus rats were markedly increased when compared with the control. Interestingly, curcumin could inhibit the upregulation of all of these biomarkers.Conclusion: These findings show that curcumin can attenuate diabetes-induced vascular dysfunction in association with its potential for COX-2 and NF-κB suppression, PKC inhibition, and improving the ratio of prostanoid products PGI2/TXA2.Keywords: diabetes, endothelial dysfunction, COX-2, prostanoids Sirada RungseesantivanonNaris ThengchaisriPreecha Ruangvejvorachaiet alDove Medical PressarticleSpecialties of internal medicineRC581-951ENDiabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Vol 2010, Iss default, Pp 421-429 (2010) |
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Specialties of internal medicine RC581-951 |
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Specialties of internal medicine RC581-951 Sirada Rungseesantivanon Naris Thengchaisri Preecha Ruangvejvorachai et al Curcumin improves prostanoid ratio in diabetic mesenteric arteries associated with cyclooxygenase-2 and NF-κB suppression |
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Sirada Rungseesantivanon1, Naris Thengchaisri4, Preecha Ruangvejvorachai2, Suthiluk Patumraj31Interdepartment of Physiology, Graduate School, 2Department of Pathology, 3Department of Physiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand; 4Department of Companion Animal Clinical Sciences, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, ThailandBackground: Curcumin, the active ingredient from turmeric rhizomes, has been shown to have a wide range of pharmacological properties including antioxidant and anti-inflammatory effects. Curcumin has been reviewed for its multiple molecular action on inhibiting tumor angiogenesis via its mechanisms of cyclooxygenase (COX)-2, and vascular endothelial growth factor (VEGF) inhibition. In this present study, we aimed to assess the effects of curcumin on preventing diabetes-induced vascular dysfunction in association with COX-2, nuclear factor-κB (NF-κB) expression, and prostanoid production.Methods: Twelve-week-old male Wistar rats were separated into five groups: 1) diabetes with 0.9% normal saline (DM-NSS; n = 10), 2) diabetes treated with curcumin 30 mg/kg (n = 10), 3) diabetes treated with curcumin 300 mg/kg (n = 10), 4) the control with 0.9% normal saline (n = 10), and 5) the control treated with 300 mg/kg (n = 10). Daily oral feeding of curcumin was started at 6 weeks after the streptozotocin injection. Levels of 6-keto prostaglandin (PG) F1α and thromboxane (TX) B2 were determined from mesenteric perfusates using enzyme immunoassay kits. Protein kinase C (PKC)-ßII and COX-2 with NF-κB levels were analyzed in the mesenteric arteries by immunofluorescent staining and immunohistochemistry, respectively.Results: The ratio of 6-keto-PGF1α and TXB2 was significantly decreased in DM-NSS compared with the control (P < 0.05). Double-immunofluorescent staining with specific antibodies for PKC-βII and a-smooth muscle actins showed that the diabetic mesenteric arteries contained increased of PKC-βII within the vascular wall. Also, COX-2 expression and activated NF-κB in the small mesenteric artery of diabetes mellitus rats were markedly increased when compared with the control. Interestingly, curcumin could inhibit the upregulation of all of these biomarkers.Conclusion: These findings show that curcumin can attenuate diabetes-induced vascular dysfunction in association with its potential for COX-2 and NF-κB suppression, PKC inhibition, and improving the ratio of prostanoid products PGI2/TXA2.Keywords: diabetes, endothelial dysfunction, COX-2, prostanoids |
| format |
article |
| author |
Sirada Rungseesantivanon Naris Thengchaisri Preecha Ruangvejvorachai et al |
| author_facet |
Sirada Rungseesantivanon Naris Thengchaisri Preecha Ruangvejvorachai et al |
| author_sort |
Sirada Rungseesantivanon |
| title |
Curcumin improves prostanoid ratio in diabetic mesenteric arteries associated with cyclooxygenase-2 and NF-κB suppression |
| title_short |
Curcumin improves prostanoid ratio in diabetic mesenteric arteries associated with cyclooxygenase-2 and NF-κB suppression |
| title_full |
Curcumin improves prostanoid ratio in diabetic mesenteric arteries associated with cyclooxygenase-2 and NF-κB suppression |
| title_fullStr |
Curcumin improves prostanoid ratio in diabetic mesenteric arteries associated with cyclooxygenase-2 and NF-κB suppression |
| title_full_unstemmed |
Curcumin improves prostanoid ratio in diabetic mesenteric arteries associated with cyclooxygenase-2 and NF-κB suppression |
| title_sort |
curcumin improves prostanoid ratio in diabetic mesenteric arteries associated with cyclooxygenase-2 and nf-κb suppression |
| publisher |
Dove Medical Press |
| publishDate |
2010 |
| url |
https://doaj.org/article/a718b8a1bcb04a7a82f517229c85302b |
| work_keys_str_mv |
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| _version_ |
1718399179226611712 |