RNA Interference Suppression of v-ATPase B and Juvenile Hormone Binding Protein Genes Through Topically Applied dsRNA on Tomato Leaves: Developing Biopesticides to Control the South American Pinworm, Tuta absoluta (Lepidoptera: Gelechiidae)

The South American pinworm Tuta absoluta (Meyrick) (Family: Gelechiidae) is one of the most devastating lepidopteran pests in the developing countries of South America, Africa, and Asia. This pest is classified as the most serious threat for tomato production worldwide. In the present study, we anal...

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Autores principales:	Govindaraju Ramkumar, Ramasamy Asokan, N. R. Prasannakumar, B. Kariyanna, Sengodan Karthi, Mona S. Alwahibi, Mohamed Soliman Elshikh, Ahmed Abdel-Megeed, Aml Ghaith, Sengottayan Senthil-Nathan, Kandaswamy Kalaivani, Wayne Brian Hunter, Patcharin Krutmuang
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	tomato leaf miner v-ATPase juvenile hormone binding protein JHBP dsRNA RNAi Physiology QP1-981
Acceso en línea:	https://doaj.org/article/a71d7da2d26b4180b258c494b88f841f
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spelling	oai:doaj.org-article:a71d7da2d26b4180b258c494b88f841f2021-11-18T08:05:13ZRNA Interference Suppression of v-ATPase B and Juvenile Hormone Binding Protein Genes Through Topically Applied dsRNA on Tomato Leaves: Developing Biopesticides to Control the South American Pinworm, Tuta absoluta (Lepidoptera: Gelechiidae)1664-042X10.3389/fphys.2021.742871https://doaj.org/article/a71d7da2d26b4180b258c494b88f841f2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fphys.2021.742871/fullhttps://doaj.org/toc/1664-042XThe South American pinworm Tuta absoluta (Meyrick) (Family: Gelechiidae) is one of the most devastating lepidopteran pests in the developing countries of South America, Africa, and Asia. This pest is classified as the most serious threat for tomato production worldwide. In the present study, we analyzed RNAi-mediated control through exogenously applied dsRNA delivery on tomato. The dsRNA treatments were made to target the juvenile hormone binding protein and the v-ATPase B. Both mRNA targets were cloned, validated by sequencing, and used to produce each dsRNA. After treatments the relative transcript expression was analyzed using qRTPCR to assess to efficacy of RNAi. A leaf-dip assay was used to provide late 2nd instar larvae three feeding access periods: 24, 48, and 72 h, to evaluate the effect of gene silencing of each target. Larvae were fed tomato leaves coated with five different RNAi concentrations (10, 20, 30, 40, and 50 micrograms/centimeter-squared), that suppressed two genes (juvenile hormone protein, JHBP, and vacuolar-type adenosine triphosphatase enzyme, v-ATPase). Treatments with dsRNA showed a significant increase in mortality at 24, 48, and 72 h after ingestion (P < 0.01, α = 0.05), along with reduced leaf damage, and increased feeding deterrence. The results suggest that these two RNAi products may provide a suitable treatment for control of this and other lepidopteran pests.Govindaraju RamkumarRamasamy AsokanN. R. PrasannakumarB. KariyannaSengodan KarthiMona S. AlwahibiMohamed Soliman ElshikhAhmed Abdel-MegeedAml GhaithSengottayan Senthil-NathanKandaswamy KalaivaniWayne Brian HunterPatcharin KrutmuangPatcharin KrutmuangFrontiers Media S.A.articletomato leaf minerv-ATPasejuvenile hormone binding proteinJHBPdsRNARNAiPhysiologyQP1-981ENFrontiers in Physiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	tomato leaf miner v-ATPase juvenile hormone binding protein JHBP dsRNA RNAi Physiology QP1-981
spellingShingle	tomato leaf miner v-ATPase juvenile hormone binding protein JHBP dsRNA RNAi Physiology QP1-981 Govindaraju Ramkumar Ramasamy Asokan N. R. Prasannakumar B. Kariyanna Sengodan Karthi Mona S. Alwahibi Mohamed Soliman Elshikh Ahmed Abdel-Megeed Aml Ghaith Sengottayan Senthil-Nathan Kandaswamy Kalaivani Wayne Brian Hunter Patcharin Krutmuang Patcharin Krutmuang RNA Interference Suppression of v-ATPase B and Juvenile Hormone Binding Protein Genes Through Topically Applied dsRNA on Tomato Leaves: Developing Biopesticides to Control the South American Pinworm, Tuta absoluta (Lepidoptera: Gelechiidae)
description	The South American pinworm Tuta absoluta (Meyrick) (Family: Gelechiidae) is one of the most devastating lepidopteran pests in the developing countries of South America, Africa, and Asia. This pest is classified as the most serious threat for tomato production worldwide. In the present study, we analyzed RNAi-mediated control through exogenously applied dsRNA delivery on tomato. The dsRNA treatments were made to target the juvenile hormone binding protein and the v-ATPase B. Both mRNA targets were cloned, validated by sequencing, and used to produce each dsRNA. After treatments the relative transcript expression was analyzed using qRTPCR to assess to efficacy of RNAi. A leaf-dip assay was used to provide late 2nd instar larvae three feeding access periods: 24, 48, and 72 h, to evaluate the effect of gene silencing of each target. Larvae were fed tomato leaves coated with five different RNAi concentrations (10, 20, 30, 40, and 50 micrograms/centimeter-squared), that suppressed two genes (juvenile hormone protein, JHBP, and vacuolar-type adenosine triphosphatase enzyme, v-ATPase). Treatments with dsRNA showed a significant increase in mortality at 24, 48, and 72 h after ingestion (P < 0.01, α = 0.05), along with reduced leaf damage, and increased feeding deterrence. The results suggest that these two RNAi products may provide a suitable treatment for control of this and other lepidopteran pests.
format	article
author	Govindaraju Ramkumar Ramasamy Asokan N. R. Prasannakumar B. Kariyanna Sengodan Karthi Mona S. Alwahibi Mohamed Soliman Elshikh Ahmed Abdel-Megeed Aml Ghaith Sengottayan Senthil-Nathan Kandaswamy Kalaivani Wayne Brian Hunter Patcharin Krutmuang Patcharin Krutmuang
author_facet	Govindaraju Ramkumar Ramasamy Asokan N. R. Prasannakumar B. Kariyanna Sengodan Karthi Mona S. Alwahibi Mohamed Soliman Elshikh Ahmed Abdel-Megeed Aml Ghaith Sengottayan Senthil-Nathan Kandaswamy Kalaivani Wayne Brian Hunter Patcharin Krutmuang Patcharin Krutmuang
author_sort	Govindaraju Ramkumar
title	RNA Interference Suppression of v-ATPase B and Juvenile Hormone Binding Protein Genes Through Topically Applied dsRNA on Tomato Leaves: Developing Biopesticides to Control the South American Pinworm, Tuta absoluta (Lepidoptera: Gelechiidae)
title_short	RNA Interference Suppression of v-ATPase B and Juvenile Hormone Binding Protein Genes Through Topically Applied dsRNA on Tomato Leaves: Developing Biopesticides to Control the South American Pinworm, Tuta absoluta (Lepidoptera: Gelechiidae)
title_full	RNA Interference Suppression of v-ATPase B and Juvenile Hormone Binding Protein Genes Through Topically Applied dsRNA on Tomato Leaves: Developing Biopesticides to Control the South American Pinworm, Tuta absoluta (Lepidoptera: Gelechiidae)
title_fullStr	RNA Interference Suppression of v-ATPase B and Juvenile Hormone Binding Protein Genes Through Topically Applied dsRNA on Tomato Leaves: Developing Biopesticides to Control the South American Pinworm, Tuta absoluta (Lepidoptera: Gelechiidae)
title_full_unstemmed	RNA Interference Suppression of v-ATPase B and Juvenile Hormone Binding Protein Genes Through Topically Applied dsRNA on Tomato Leaves: Developing Biopesticides to Control the South American Pinworm, Tuta absoluta (Lepidoptera: Gelechiidae)
title_sort	rna interference suppression of v-atpase b and juvenile hormone binding protein genes through topically applied dsrna on tomato leaves: developing biopesticides to control the south american pinworm, tuta absoluta (lepidoptera: gelechiidae)
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/a71d7da2d26b4180b258c494b88f841f
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RNA Interference Suppression of v-ATPase B and Juvenile Hormone Binding Protein Genes Through Topically Applied dsRNA on Tomato Leaves: Developing Biopesticides to Control the South American Pinworm, Tuta absoluta (Lepidoptera: Gelechiidae)

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