Reversible Signal Binding by the <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content> Quorum-Sensing Signal Receptor LasR

ABSTRACT Many members of the LuxR family of acyl-homoserine lactone (acyl-HSL)-dependent quorum-sensing transcriptional activators are thought to have the unusual characteristics of requiring the signal ligand during polypeptide synthesis to fold into an active conformation and of binding signal ex...

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Autores principales:	Kaia J. Sappington, Ajai A. Dandekar, Ken-Ichi Oinuma, E. Peter Greenberg
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2011
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/a71f810e1e4f474cae352faa96b9de16
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spelling	oai:doaj.org-article:a71f810e1e4f474cae352faa96b9de162021-11-15T15:38:46ZReversible Signal Binding by the <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content> Quorum-Sensing Signal Receptor LasR10.1128/mBio.00011-112150-7511https://doaj.org/article/a71f810e1e4f474cae352faa96b9de162011-03-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00011-11https://doaj.org/toc/2150-7511ABSTRACT Many members of the LuxR family of acyl-homoserine lactone (acyl-HSL)-dependent quorum-sensing transcriptional activators are thought to have the unusual characteristics of requiring the signal ligand during polypeptide synthesis to fold into an active conformation and of binding signal extraordinarily tightly. This is the case for the N-3-oxo-dodecanoyl-HSL-dependent Pseudomonas aeruginosa virulence regulator LasR. We present evidence that LasR can fold into an active conformation in vivo in the absence of the acyl-HSL ligand. We also present evidence indicating that in the cellular environment, LasR and N-3-oxo-dodecanoyl-HSL readily dissociate. After dissociation, LasR can remain in a properly folded conformation capable of reassociating with signal. We present a new model for the folding and signal binding of LasR and other members of the family of transcription factors to which LasR belongs. Our findings have important implications concerning the cellular responses to decreased environmental concentrations of signals and have implications about potential quorum-sensing inhibition strategies. IMPORTANCE The opportunistic pathogen Pseudomonas aeruginosa causes difficult to treat and incurable diseases. Quorum sensing controls expression of many virulence factors in this bacterium, and the quorum-sensing signal receptor LasR has been targeted for anti-quorum-sensing therapeutic development. The work described here changes the current view about how LasR interacts with the quorum-sensing signal to which it responds. This is of importance for therapeutic development. The experiments also address a poorly studied aspect of quorum sensing: the response to decreases in quorum-sensing signals. This has importance with respect to understanding the ecology and evolution of quorum sensing.Kaia J. SappingtonAjai A. DandekarKen-Ichi OinumaE. Peter GreenbergAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 2, Iss 1 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Kaia J. Sappington Ajai A. Dandekar Ken-Ichi Oinuma E. Peter Greenberg Reversible Signal Binding by the <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content> Quorum-Sensing Signal Receptor LasR
description	ABSTRACT Many members of the LuxR family of acyl-homoserine lactone (acyl-HSL)-dependent quorum-sensing transcriptional activators are thought to have the unusual characteristics of requiring the signal ligand during polypeptide synthesis to fold into an active conformation and of binding signal extraordinarily tightly. This is the case for the N-3-oxo-dodecanoyl-HSL-dependent Pseudomonas aeruginosa virulence regulator LasR. We present evidence that LasR can fold into an active conformation in vivo in the absence of the acyl-HSL ligand. We also present evidence indicating that in the cellular environment, LasR and N-3-oxo-dodecanoyl-HSL readily dissociate. After dissociation, LasR can remain in a properly folded conformation capable of reassociating with signal. We present a new model for the folding and signal binding of LasR and other members of the family of transcription factors to which LasR belongs. Our findings have important implications concerning the cellular responses to decreased environmental concentrations of signals and have implications about potential quorum-sensing inhibition strategies. IMPORTANCE The opportunistic pathogen Pseudomonas aeruginosa causes difficult to treat and incurable diseases. Quorum sensing controls expression of many virulence factors in this bacterium, and the quorum-sensing signal receptor LasR has been targeted for anti-quorum-sensing therapeutic development. The work described here changes the current view about how LasR interacts with the quorum-sensing signal to which it responds. This is of importance for therapeutic development. The experiments also address a poorly studied aspect of quorum sensing: the response to decreases in quorum-sensing signals. This has importance with respect to understanding the ecology and evolution of quorum sensing.
format	article
author	Kaia J. Sappington Ajai A. Dandekar Ken-Ichi Oinuma E. Peter Greenberg
author_facet	Kaia J. Sappington Ajai A. Dandekar Ken-Ichi Oinuma E. Peter Greenberg
author_sort	Kaia J. Sappington
title	Reversible Signal Binding by the <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content> Quorum-Sensing Signal Receptor LasR
title_short	Reversible Signal Binding by the <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content> Quorum-Sensing Signal Receptor LasR
title_full	Reversible Signal Binding by the <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content> Quorum-Sensing Signal Receptor LasR
title_fullStr	Reversible Signal Binding by the <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content> Quorum-Sensing Signal Receptor LasR
title_full_unstemmed	Reversible Signal Binding by the <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content> Quorum-Sensing Signal Receptor LasR
title_sort	reversible signal binding by the <named-content content-type="genus-species">pseudomonas aeruginosa</named-content> quorum-sensing signal receptor lasr
publisher	American Society for Microbiology
publishDate	2011
url	https://doaj.org/article/a71f810e1e4f474cae352faa96b9de16
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Reversible Signal Binding by the <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content> Quorum-Sensing Signal Receptor LasR

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