The diagnostic accuracy of isothermal nucleic acid point-of-care tests for human coronaviruses: A systematic review and meta-analysis

Abstract Many recent studies reported coronavirus point-of-care tests (POCTs) based on isothermal amplification. However, the performances of these tests have not been systematically evaluated. Cochrane Handbook for Systematic Reviews of Diagnostic Test Accuracy was used as a guideline for conductin...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Pakpoom Subsoontorn, Manupat Lohitnavy, Chuenjid Kongkaew
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a720109411a849fab6a26b9799a46ccd
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a720109411a849fab6a26b9799a46ccd
record_format	dspace
spelling	oai:doaj.org-article:a720109411a849fab6a26b9799a46ccd2021-12-02T11:57:58ZThe diagnostic accuracy of isothermal nucleic acid point-of-care tests for human coronaviruses: A systematic review and meta-analysis10.1038/s41598-020-79237-72045-2322https://doaj.org/article/a720109411a849fab6a26b9799a46ccd2020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79237-7https://doaj.org/toc/2045-2322Abstract Many recent studies reported coronavirus point-of-care tests (POCTs) based on isothermal amplification. However, the performances of these tests have not been systematically evaluated. Cochrane Handbook for Systematic Reviews of Diagnostic Test Accuracy was used as a guideline for conducting this systematic review. We searched peer-reviewed and preprint articles in PubMed, BioRxiv and MedRxiv up to 28 September 2020 to identify studies that provide data to calculate sensitivity, specificity and diagnostic odds ratio (DOR). Quality Assessment of Diagnostic Accuracy Studies 2 (QUADAS-2) was applied for assessing quality of included studies and Preferred Reporting Items for a Systematic Review and Meta-analysis of Diagnostic Test Accuracy Studies (PRISMA-DTA) was followed for reporting. We included 81 studies from 65 research articles on POCTs of SARS, MERS and COVID-19. Most studies had high risk of patient selection and index test bias but low risk in other domains. Diagnostic specificities were high (> 0.95) for included studies while sensitivities varied depending on type of assays and sample used. Most studies (n = 51) used reverse transcription loop-mediated isothermal amplification (RT-LAMP) to diagnose coronaviruses. RT-LAMP of RNA purified from COVID-19 patient samples had pooled sensitivity at 0.94 (95% CI: 0.90–0.96). RT-LAMP of crude samples had substantially lower sensitivity at 0.78 (95% CI: 0.65–0.87). Abbott ID Now performance was similar to RT-LAMP of crude samples. Diagnostic performances by CRISPR and RT-LAMP on purified RNA were similar. Other diagnostic platforms including RT- recombinase assisted amplification (RT-RAA) and SAMBA-II also offered high sensitivity (> 0.95). Future studies should focus on the use of un-bias patient cohorts, double-blinded index test and detection assays that do not require RNA extraction.Pakpoom SubsoontornManupat LohitnavyChuenjid KongkaewNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-13 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Pakpoom Subsoontorn Manupat Lohitnavy Chuenjid Kongkaew The diagnostic accuracy of isothermal nucleic acid point-of-care tests for human coronaviruses: A systematic review and meta-analysis
description	Abstract Many recent studies reported coronavirus point-of-care tests (POCTs) based on isothermal amplification. However, the performances of these tests have not been systematically evaluated. Cochrane Handbook for Systematic Reviews of Diagnostic Test Accuracy was used as a guideline for conducting this systematic review. We searched peer-reviewed and preprint articles in PubMed, BioRxiv and MedRxiv up to 28 September 2020 to identify studies that provide data to calculate sensitivity, specificity and diagnostic odds ratio (DOR). Quality Assessment of Diagnostic Accuracy Studies 2 (QUADAS-2) was applied for assessing quality of included studies and Preferred Reporting Items for a Systematic Review and Meta-analysis of Diagnostic Test Accuracy Studies (PRISMA-DTA) was followed for reporting. We included 81 studies from 65 research articles on POCTs of SARS, MERS and COVID-19. Most studies had high risk of patient selection and index test bias but low risk in other domains. Diagnostic specificities were high (> 0.95) for included studies while sensitivities varied depending on type of assays and sample used. Most studies (n = 51) used reverse transcription loop-mediated isothermal amplification (RT-LAMP) to diagnose coronaviruses. RT-LAMP of RNA purified from COVID-19 patient samples had pooled sensitivity at 0.94 (95% CI: 0.90–0.96). RT-LAMP of crude samples had substantially lower sensitivity at 0.78 (95% CI: 0.65–0.87). Abbott ID Now performance was similar to RT-LAMP of crude samples. Diagnostic performances by CRISPR and RT-LAMP on purified RNA were similar. Other diagnostic platforms including RT- recombinase assisted amplification (RT-RAA) and SAMBA-II also offered high sensitivity (> 0.95). Future studies should focus on the use of un-bias patient cohorts, double-blinded index test and detection assays that do not require RNA extraction.
format	article
author	Pakpoom Subsoontorn Manupat Lohitnavy Chuenjid Kongkaew
author_facet	Pakpoom Subsoontorn Manupat Lohitnavy Chuenjid Kongkaew
author_sort	Pakpoom Subsoontorn
title	The diagnostic accuracy of isothermal nucleic acid point-of-care tests for human coronaviruses: A systematic review and meta-analysis
title_short	The diagnostic accuracy of isothermal nucleic acid point-of-care tests for human coronaviruses: A systematic review and meta-analysis
title_full	The diagnostic accuracy of isothermal nucleic acid point-of-care tests for human coronaviruses: A systematic review and meta-analysis
title_fullStr	The diagnostic accuracy of isothermal nucleic acid point-of-care tests for human coronaviruses: A systematic review and meta-analysis
title_full_unstemmed	The diagnostic accuracy of isothermal nucleic acid point-of-care tests for human coronaviruses: A systematic review and meta-analysis
title_sort	diagnostic accuracy of isothermal nucleic acid point-of-care tests for human coronaviruses: a systematic review and meta-analysis
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/a720109411a849fab6a26b9799a46ccd
work_keys_str_mv	AT pakpoomsubsoontorn thediagnosticaccuracyofisothermalnucleicacidpointofcaretestsforhumancoronavirusesasystematicreviewandmetaanalysis AT manupatlohitnavy thediagnosticaccuracyofisothermalnucleicacidpointofcaretestsforhumancoronavirusesasystematicreviewandmetaanalysis AT chuenjidkongkaew thediagnosticaccuracyofisothermalnucleicacidpointofcaretestsforhumancoronavirusesasystematicreviewandmetaanalysis AT pakpoomsubsoontorn diagnosticaccuracyofisothermalnucleicacidpointofcaretestsforhumancoronavirusesasystematicreviewandmetaanalysis AT manupatlohitnavy diagnosticaccuracyofisothermalnucleicacidpointofcaretestsforhumancoronavirusesasystematicreviewandmetaanalysis AT chuenjidkongkaew diagnosticaccuracyofisothermalnucleicacidpointofcaretestsforhumancoronavirusesasystematicreviewandmetaanalysis
_version_	1718394767368257536

The diagnostic accuracy of isothermal nucleic acid point-of-care tests for human coronaviruses: A systematic review and meta-analysis

Ejemplares similares