Spectral characteristics of voltage-sensitive indocyanine green fluorescence in the heart

Abstract Indocyanine green (ICG) fluorescent dye has been approved by the FDA for use in medical diagnostics. Recently, we demonstrated that ICG dye has voltage-sensitive properties with a dual-component (fast and slow) response in the Langendorff-perfused rabbit heart. Here, we extended our studies...

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Autores principales:	Regina Mačianskienė, Mantė Almanaitytė, Rimantas Treinys, Antanas Navalinskas, Rimantas Benetis, Jonas Jurevičius
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a721c7140429411aa78e7365214ea1ce
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spelling	oai:doaj.org-article:a721c7140429411aa78e7365214ea1ce2021-12-02T16:06:17ZSpectral characteristics of voltage-sensitive indocyanine green fluorescence in the heart10.1038/s41598-017-08168-72045-2322https://doaj.org/article/a721c7140429411aa78e7365214ea1ce2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08168-7https://doaj.org/toc/2045-2322Abstract Indocyanine green (ICG) fluorescent dye has been approved by the FDA for use in medical diagnostics. Recently, we demonstrated that ICG dye has voltage-sensitive properties with a dual-component (fast and slow) response in the Langendorff-perfused rabbit heart. Here, we extended our studies by showing the different spectral properties of both components for analysis of the fractional change in ICG fluorescence in response to voltage changes. We used light from four LEDs to obtain excitation; emission was measured using an EMCCD camera with band-pass filters and a spectrometer. We applied a graphical model with Gaussian functions to construct and evaluate the individual emission curves and calculated the voltage-sensitive portion of each component of the ICG fluorescence in the rabbit heart. The results revealed that each isolated component (fast and slow) emanates from a unique ICG pool in a different environment within the cell membrane and that each component is also composed of two constituents (ICG-monomeric and ICG-aggregated). We propose the existence of different voltage-sensitive mechanisms for the components: (I) electrochromism and field-induced reorientation for the fast component; and (II) field-induced dye squeezing that amplifies intermolecular interactions, resulting in self-quenching of the dye fluorescence, for the slow component.Regina MačianskienėMantė AlmanaitytėRimantas TreinysAntanas NavalinskasRimantas BenetisJonas JurevičiusNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Regina Mačianskienė Mantė Almanaitytė Rimantas Treinys Antanas Navalinskas Rimantas Benetis Jonas Jurevičius Spectral characteristics of voltage-sensitive indocyanine green fluorescence in the heart
description	Abstract Indocyanine green (ICG) fluorescent dye has been approved by the FDA for use in medical diagnostics. Recently, we demonstrated that ICG dye has voltage-sensitive properties with a dual-component (fast and slow) response in the Langendorff-perfused rabbit heart. Here, we extended our studies by showing the different spectral properties of both components for analysis of the fractional change in ICG fluorescence in response to voltage changes. We used light from four LEDs to obtain excitation; emission was measured using an EMCCD camera with band-pass filters and a spectrometer. We applied a graphical model with Gaussian functions to construct and evaluate the individual emission curves and calculated the voltage-sensitive portion of each component of the ICG fluorescence in the rabbit heart. The results revealed that each isolated component (fast and slow) emanates from a unique ICG pool in a different environment within the cell membrane and that each component is also composed of two constituents (ICG-monomeric and ICG-aggregated). We propose the existence of different voltage-sensitive mechanisms for the components: (I) electrochromism and field-induced reorientation for the fast component; and (II) field-induced dye squeezing that amplifies intermolecular interactions, resulting in self-quenching of the dye fluorescence, for the slow component.
format	article
author	Regina Mačianskienė Mantė Almanaitytė Rimantas Treinys Antanas Navalinskas Rimantas Benetis Jonas Jurevičius
author_facet	Regina Mačianskienė Mantė Almanaitytė Rimantas Treinys Antanas Navalinskas Rimantas Benetis Jonas Jurevičius
author_sort	Regina Mačianskienė
title	Spectral characteristics of voltage-sensitive indocyanine green fluorescence in the heart
title_short	Spectral characteristics of voltage-sensitive indocyanine green fluorescence in the heart
title_full	Spectral characteristics of voltage-sensitive indocyanine green fluorescence in the heart
title_fullStr	Spectral characteristics of voltage-sensitive indocyanine green fluorescence in the heart
title_full_unstemmed	Spectral characteristics of voltage-sensitive indocyanine green fluorescence in the heart
title_sort	spectral characteristics of voltage-sensitive indocyanine green fluorescence in the heart
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/a721c7140429411aa78e7365214ea1ce
work_keys_str_mv	AT reginamacianskiene spectralcharacteristicsofvoltagesensitiveindocyaninegreenfluorescenceintheheart AT mantealmanaityte spectralcharacteristicsofvoltagesensitiveindocyaninegreenfluorescenceintheheart AT rimantastreinys spectralcharacteristicsofvoltagesensitiveindocyaninegreenfluorescenceintheheart AT antanasnavalinskas spectralcharacteristicsofvoltagesensitiveindocyaninegreenfluorescenceintheheart AT rimantasbenetis spectralcharacteristicsofvoltagesensitiveindocyaninegreenfluorescenceintheheart AT jonasjurevicius spectralcharacteristicsofvoltagesensitiveindocyaninegreenfluorescenceintheheart
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Spectral characteristics of voltage-sensitive indocyanine green fluorescence in the heart

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