Development of Plant–Fungal Endophyte Associations to Suppress Phoma Stem Canker in <i>Brassica</i>

Endophytic microorganisms are found within the tissues of many plants species, with some conferring several benefits to the host plant including resistance to plant diseases. In this study, two putative endophytic fungi that were previously isolated from wild seeds of <i>Brassica</i>, id...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Davood Roodi, James P. Millner, Craig R. McGill, Richard D. Johnson, Shen-Yan Hea, Jenny J. Brookes, Travis R. Glare, Stuart D. Card
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Beauveria bassiana</i> biological control brassicaceae cordycipitaceae leptosphaeriaceae <i>Leptosphaeria maculans</i> Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/a72dd5d17f684de691ece20b4cf70461
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a72dd5d17f684de691ece20b4cf70461
record_format	dspace
spelling	oai:doaj.org-article:a72dd5d17f684de691ece20b4cf704612021-11-25T18:25:36ZDevelopment of Plant–Fungal Endophyte Associations to Suppress Phoma Stem Canker in <i>Brassica</i>10.3390/microorganisms91123872076-2607https://doaj.org/article/a72dd5d17f684de691ece20b4cf704612021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2387https://doaj.org/toc/2076-2607Endophytic microorganisms are found within the tissues of many plants species, with some conferring several benefits to the host plant including resistance to plant diseases. In this study, two putative endophytic fungi that were previously isolated from wild seeds of <i>Brassica</i>, identified as <i>Beauveria bassiana</i> and <i>Pseudogymnoascus pannorum</i>, were inoculated into cultivars of three <i>Brassica</i> species—<i>Brassica napus</i>, <i>Br. rapa</i> and <i>Br. oleracea</i>. Both fungal endophytes were reisolated from above- and below-ground tissues of inoculated plants at four different plant-growth stages, including cotyledon, one-leaf, two-leaf, and four-leaf stages. None of the plants colonised by these fungi exhibited any obvious disease symptoms, indicating the formation of novel mutualistic associations. These novel plant–endophyte associations formed between <i>Brassica</i> plants and <i>Be. bassiana</i> significantly inhibited phoma stem canker, a devastating disease of <i>Brassica</i> crops worldwide, caused by the fungal pathogen <i>Leptosphaeria maculans</i>. The novel association formed with <i>P. pannorum</i> significantly suppressed the amount of disease caused by <i>L. maculans</i> in one out of two experiments. Although biological control is not a new strategy, endophytic fungi with both antiinsect and antifungal activity are a highly conceivable, sustainable option to manage pests and diseases of economically important crops.Davood RoodiJames P. MillnerCraig R. McGillRichard D. JohnsonShen-Yan HeaJenny J. BrookesTravis R. GlareStuart D. CardMDPI AGarticle<i>Beauveria bassiana</i>biological controlbrassicaceaecordycipitaceaeleptosphaeriaceae<i>Leptosphaeria maculans</i>Biology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2387, p 2387 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Beauveria bassiana</i> biological control brassicaceae cordycipitaceae leptosphaeriaceae <i>Leptosphaeria maculans</i> Biology (General) QH301-705.5
spellingShingle	<i>Beauveria bassiana</i> biological control brassicaceae cordycipitaceae leptosphaeriaceae <i>Leptosphaeria maculans</i> Biology (General) QH301-705.5 Davood Roodi James P. Millner Craig R. McGill Richard D. Johnson Shen-Yan Hea Jenny J. Brookes Travis R. Glare Stuart D. Card Development of Plant–Fungal Endophyte Associations to Suppress Phoma Stem Canker in <i>Brassica</i>
description	Endophytic microorganisms are found within the tissues of many plants species, with some conferring several benefits to the host plant including resistance to plant diseases. In this study, two putative endophytic fungi that were previously isolated from wild seeds of <i>Brassica</i>, identified as <i>Beauveria bassiana</i> and <i>Pseudogymnoascus pannorum</i>, were inoculated into cultivars of three <i>Brassica</i> species—<i>Brassica napus</i>, <i>Br. rapa</i> and <i>Br. oleracea</i>. Both fungal endophytes were reisolated from above- and below-ground tissues of inoculated plants at four different plant-growth stages, including cotyledon, one-leaf, two-leaf, and four-leaf stages. None of the plants colonised by these fungi exhibited any obvious disease symptoms, indicating the formation of novel mutualistic associations. These novel plant–endophyte associations formed between <i>Brassica</i> plants and <i>Be. bassiana</i> significantly inhibited phoma stem canker, a devastating disease of <i>Brassica</i> crops worldwide, caused by the fungal pathogen <i>Leptosphaeria maculans</i>. The novel association formed with <i>P. pannorum</i> significantly suppressed the amount of disease caused by <i>L. maculans</i> in one out of two experiments. Although biological control is not a new strategy, endophytic fungi with both antiinsect and antifungal activity are a highly conceivable, sustainable option to manage pests and diseases of economically important crops.
format	article
author	Davood Roodi James P. Millner Craig R. McGill Richard D. Johnson Shen-Yan Hea Jenny J. Brookes Travis R. Glare Stuart D. Card
author_facet	Davood Roodi James P. Millner Craig R. McGill Richard D. Johnson Shen-Yan Hea Jenny J. Brookes Travis R. Glare Stuart D. Card
author_sort	Davood Roodi
title	Development of Plant–Fungal Endophyte Associations to Suppress Phoma Stem Canker in <i>Brassica</i>
title_short	Development of Plant–Fungal Endophyte Associations to Suppress Phoma Stem Canker in <i>Brassica</i>
title_full	Development of Plant–Fungal Endophyte Associations to Suppress Phoma Stem Canker in <i>Brassica</i>
title_fullStr	Development of Plant–Fungal Endophyte Associations to Suppress Phoma Stem Canker in <i>Brassica</i>
title_full_unstemmed	Development of Plant–Fungal Endophyte Associations to Suppress Phoma Stem Canker in <i>Brassica</i>
title_sort	development of plant–fungal endophyte associations to suppress phoma stem canker in <i>brassica</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/a72dd5d17f684de691ece20b4cf70461
work_keys_str_mv	AT davoodroodi developmentofplantfungalendophyteassociationstosuppressphomastemcankerinibrassicai AT jamespmillner developmentofplantfungalendophyteassociationstosuppressphomastemcankerinibrassicai AT craigrmcgill developmentofplantfungalendophyteassociationstosuppressphomastemcankerinibrassicai AT richarddjohnson developmentofplantfungalendophyteassociationstosuppressphomastemcankerinibrassicai AT shenyanhea developmentofplantfungalendophyteassociationstosuppressphomastemcankerinibrassicai AT jennyjbrookes developmentofplantfungalendophyteassociationstosuppressphomastemcankerinibrassicai AT travisrglare developmentofplantfungalendophyteassociationstosuppressphomastemcankerinibrassicai AT stuartdcard developmentofplantfungalendophyteassociationstosuppressphomastemcankerinibrassicai
_version_	1718411214714830848

Development of Plant–Fungal Endophyte Associations to Suppress Phoma Stem Canker in <i>Brassica</i>

Ejemplares similares