EFFICIENCY OF APPLICATION OF ISOTHERMAL AMPLIFICATION AT INSPECTION OF PATIENTS WITH WHOOPING COUGH

Purpose: efficiency isothermal amplification (LAMP) at inspection of patients with whooping cough in clinical conditions. Materials and methods. Examination of 262 patients aged from 0 months up to 30 years hospitalized in Infectious diseases clinical hospital No. 1 of the Moscow Department of Healt...

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Autores principales:	A. S. Pimenova, O. Yu. Borisova, M. S. Petrova, I. S. Voronina, A. B. Borisova, O. V. Shamsheva, S. S. Afanasiev, E. V. Vlasov, V. A. Aleshkin
Formato:	article
Lenguaje:	RU
Publicado:	Sankt-Peterburg : NIIÈM imeni Pastera 2018
Materias:	whooping cough isothermal amplification patients age period of disease Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/a7310805f37a425198e1ea853e50c079
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spelling	oai:doaj.org-article:a7310805f37a425198e1ea853e50c0792021-11-22T07:09:49ZEFFICIENCY OF APPLICATION OF ISOTHERMAL AMPLIFICATION AT INSPECTION OF PATIENTS WITH WHOOPING COUGH2220-76192313-739810.15789/2220-7619-2018-3-361-368https://doaj.org/article/a7310805f37a425198e1ea853e50c0792018-11-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/788https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398Purpose: efficiency isothermal amplification (LAMP) at inspection of patients with whooping cough in clinical conditions. Materials and methods. Examination of 262 patients aged from 0 months up to 30 years hospitalized in Infectious diseases clinical hospital No. 1 of the Moscow Department of Healthcare is conducted. Clinical specimens (pharyngeal swabs) were collected according to MR 3.1.2.0072-13. Extraction DNA of B. pertussiswas carried out by means of the АmplyPrime® DNA-sorb-AM. Identification of specific fragments of a genome of B. pertussiswas performed by PCR-real time by means of the АmplySens ® Bordetella Multi-FL set (a comparison method) and by the LAMP with a phoresis and intercalating dye. Results.When using of the optimized method LAMP the DNA of B. pertussisis found at 252 (96.2%) patients. The method was effective at any forms of whooping cough —DNA of B. pertussisis found in all patients with a severe form, in 95.8% of cases — in patients with medium-weight and in 95.3% of cases — in patients with an easy form. The DNA of B. pertussisis found in clinical specimens received from patients on different terms from the beginning of a disease — from 92.3% on the 1st week up to 96% of cases — on the 5 th and more weeks of a disease. The DNA of B. pertussisis found in high percent of cases (96.7–95.9%) and did not depend on acceptance of antibacterial therapy. Children till 1 year are the main age group which is subject to hospitalization at suspicion of whooping cough and in which the highest risk of development of complications and severe forms of a clinical disease. At inspection of 169 children from 0 to 12 months by means of the optimized method LAMP, DNA of B. pertussisit is found in 98.6% of cases in children of patients with whooping cough aged from 0–3 months, in 98.4% of cases — children of 4–7 months and in 94.6% of cases — at children have 8–12 months. The efficiency of detection of DNA of B. pertussisat patients with whooping cough of children aged till 1 year by means of the optimized LAMP method was 97.6%.A. S. PimenovaO. Yu. BorisovaM. S. PetrovaI. S. VoroninaA. B. BorisovaO. V. ShamshevaS. S. AfanasievE. V. VlasovV. A. AleshkinSankt-Peterburg : NIIÈM imeni Pasteraarticlewhooping coughisothermal amplificationpatientsageperiod of diseaseInfectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 8, Iss 3, Pp 361-368 (2018)
institution	DOAJ
collection	DOAJ
language	RU
topic	whooping cough isothermal amplification patients age period of disease Infectious and parasitic diseases RC109-216
spellingShingle	whooping cough isothermal amplification patients age period of disease Infectious and parasitic diseases RC109-216 A. S. Pimenova O. Yu. Borisova M. S. Petrova I. S. Voronina A. B. Borisova O. V. Shamsheva S. S. Afanasiev E. V. Vlasov V. A. Aleshkin EFFICIENCY OF APPLICATION OF ISOTHERMAL AMPLIFICATION AT INSPECTION OF PATIENTS WITH WHOOPING COUGH
description	Purpose: efficiency isothermal amplification (LAMP) at inspection of patients with whooping cough in clinical conditions. Materials and methods. Examination of 262 patients aged from 0 months up to 30 years hospitalized in Infectious diseases clinical hospital No. 1 of the Moscow Department of Healthcare is conducted. Clinical specimens (pharyngeal swabs) were collected according to MR 3.1.2.0072-13. Extraction DNA of B. pertussiswas carried out by means of the АmplyPrime® DNA-sorb-AM. Identification of specific fragments of a genome of B. pertussiswas performed by PCR-real time by means of the АmplySens ® Bordetella Multi-FL set (a comparison method) and by the LAMP with a phoresis and intercalating dye. Results.When using of the optimized method LAMP the DNA of B. pertussisis found at 252 (96.2%) patients. The method was effective at any forms of whooping cough —DNA of B. pertussisis found in all patients with a severe form, in 95.8% of cases — in patients with medium-weight and in 95.3% of cases — in patients with an easy form. The DNA of B. pertussisis found in clinical specimens received from patients on different terms from the beginning of a disease — from 92.3% on the 1st week up to 96% of cases — on the 5 th and more weeks of a disease. The DNA of B. pertussisis found in high percent of cases (96.7–95.9%) and did not depend on acceptance of antibacterial therapy. Children till 1 year are the main age group which is subject to hospitalization at suspicion of whooping cough and in which the highest risk of development of complications and severe forms of a clinical disease. At inspection of 169 children from 0 to 12 months by means of the optimized method LAMP, DNA of B. pertussisit is found in 98.6% of cases in children of patients with whooping cough aged from 0–3 months, in 98.4% of cases — children of 4–7 months and in 94.6% of cases — at children have 8–12 months. The efficiency of detection of DNA of B. pertussisat patients with whooping cough of children aged till 1 year by means of the optimized LAMP method was 97.6%.
format	article
author	A. S. Pimenova O. Yu. Borisova M. S. Petrova I. S. Voronina A. B. Borisova O. V. Shamsheva S. S. Afanasiev E. V. Vlasov V. A. Aleshkin
author_facet	A. S. Pimenova O. Yu. Borisova M. S. Petrova I. S. Voronina A. B. Borisova O. V. Shamsheva S. S. Afanasiev E. V. Vlasov V. A. Aleshkin
author_sort	A. S. Pimenova
title	EFFICIENCY OF APPLICATION OF ISOTHERMAL AMPLIFICATION AT INSPECTION OF PATIENTS WITH WHOOPING COUGH
title_short	EFFICIENCY OF APPLICATION OF ISOTHERMAL AMPLIFICATION AT INSPECTION OF PATIENTS WITH WHOOPING COUGH
title_full	EFFICIENCY OF APPLICATION OF ISOTHERMAL AMPLIFICATION AT INSPECTION OF PATIENTS WITH WHOOPING COUGH
title_fullStr	EFFICIENCY OF APPLICATION OF ISOTHERMAL AMPLIFICATION AT INSPECTION OF PATIENTS WITH WHOOPING COUGH
title_full_unstemmed	EFFICIENCY OF APPLICATION OF ISOTHERMAL AMPLIFICATION AT INSPECTION OF PATIENTS WITH WHOOPING COUGH
title_sort	efficiency of application of isothermal amplification at inspection of patients with whooping cough
publisher	Sankt-Peterburg : NIIÈM imeni Pastera
publishDate	2018
url	https://doaj.org/article/a7310805f37a425198e1ea853e50c079
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EFFICIENCY OF APPLICATION OF ISOTHERMAL AMPLIFICATION AT INSPECTION OF PATIENTS WITH WHOOPING COUGH

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