Non-ionising UV light increases the optical density of hygroscopic self assembled DNA crystal films

Abstract We report on ultraviolet (UV) light induced increases in the UV optical density of thin and optically transparent crystalline DNA films formed through self assembly. The films are comprised of closely packed, multi-faceted and sub micron sized crystals. UV-Vis spectrophotometry reveals that...

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Autores principales:	Alexandria E. Gasperini, Susy Sanchez, Amber L. Doiron, Mark Lyles, Guy K. German
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a7393b5d2aff408ba86466dd4ce2d2d5
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spelling	oai:doaj.org-article:a7393b5d2aff408ba86466dd4ce2d2d52021-12-02T15:06:14ZNon-ionising UV light increases the optical density of hygroscopic self assembled DNA crystal films10.1038/s41598-017-06884-82045-2322https://doaj.org/article/a7393b5d2aff408ba86466dd4ce2d2d52017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06884-8https://doaj.org/toc/2045-2322Abstract We report on ultraviolet (UV) light induced increases in the UV optical density of thin and optically transparent crystalline DNA films formed through self assembly. The films are comprised of closely packed, multi-faceted and sub micron sized crystals. UV-Vis spectrophotometry reveals that DNA films with surface densities up to 0.031 mg/mm2 can reduce the transmittance of incident UVC and UVB light by up to 90%, and UVA transmittance by up to 20%. Subsequent and independent film irradiation with either UVA or UVB dosages upwards of 80 J/cm2 both reduce UV transmittance, with reductions scaling monotonically with UV dosage. To date the induction of a hyperchromic effect has been demonstrated using heat, pH, high salt mediums, and high energy ionising radiation. Both hyperchromicity and increased light scattering could account for the increased film optical density after UV irradiation. Additional characterisation of the films reveal they are highly absorbent and hygroscopic. When coated on human skin, they are capable of slowing water evaporation and keeping the tissue hydrated for extended periods of time.Alexandria E. GasperiniSusy SanchezAmber L. DoironMark LylesGuy K. GermanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Alexandria E. Gasperini Susy Sanchez Amber L. Doiron Mark Lyles Guy K. German Non-ionising UV light increases the optical density of hygroscopic self assembled DNA crystal films
description	Abstract We report on ultraviolet (UV) light induced increases in the UV optical density of thin and optically transparent crystalline DNA films formed through self assembly. The films are comprised of closely packed, multi-faceted and sub micron sized crystals. UV-Vis spectrophotometry reveals that DNA films with surface densities up to 0.031 mg/mm2 can reduce the transmittance of incident UVC and UVB light by up to 90%, and UVA transmittance by up to 20%. Subsequent and independent film irradiation with either UVA or UVB dosages upwards of 80 J/cm2 both reduce UV transmittance, with reductions scaling monotonically with UV dosage. To date the induction of a hyperchromic effect has been demonstrated using heat, pH, high salt mediums, and high energy ionising radiation. Both hyperchromicity and increased light scattering could account for the increased film optical density after UV irradiation. Additional characterisation of the films reveal they are highly absorbent and hygroscopic. When coated on human skin, they are capable of slowing water evaporation and keeping the tissue hydrated for extended periods of time.
format	article
author	Alexandria E. Gasperini Susy Sanchez Amber L. Doiron Mark Lyles Guy K. German
author_facet	Alexandria E. Gasperini Susy Sanchez Amber L. Doiron Mark Lyles Guy K. German
author_sort	Alexandria E. Gasperini
title	Non-ionising UV light increases the optical density of hygroscopic self assembled DNA crystal films
title_short	Non-ionising UV light increases the optical density of hygroscopic self assembled DNA crystal films
title_full	Non-ionising UV light increases the optical density of hygroscopic self assembled DNA crystal films
title_fullStr	Non-ionising UV light increases the optical density of hygroscopic self assembled DNA crystal films
title_full_unstemmed	Non-ionising UV light increases the optical density of hygroscopic self assembled DNA crystal films
title_sort	non-ionising uv light increases the optical density of hygroscopic self assembled dna crystal films
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/a7393b5d2aff408ba86466dd4ce2d2d5
work_keys_str_mv	AT alexandriaegasperini nonionisinguvlightincreasestheopticaldensityofhygroscopicselfassembleddnacrystalfilms AT susysanchez nonionisinguvlightincreasestheopticaldensityofhygroscopicselfassembleddnacrystalfilms AT amberldoiron nonionisinguvlightincreasestheopticaldensityofhygroscopicselfassembleddnacrystalfilms AT marklyles nonionisinguvlightincreasestheopticaldensityofhygroscopicselfassembleddnacrystalfilms AT guykgerman nonionisinguvlightincreasestheopticaldensityofhygroscopicselfassembleddnacrystalfilms
_version_	1718388535303602176

Non-ionising UV light increases the optical density of hygroscopic self assembled DNA crystal films

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