Mycoviruses of an endophytic fungus can replicate in plant cells: evolutionary implications

Abstract So far there is no record of a specific virus able to infect both fungal and plant hosts in nature. However, experimental evidence shows that some plant virus RdRPs are able to perform replication in trans of genomic or DI RNAs in the yeast Saccharomyces cerevisiae. Furthermore, tobacco mos...

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Autores principales:	L. Nerva, G. C. Varese, B. W. Falk, M. Turina
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a73bdc2102e147b697feb2e838a9014f
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spelling	oai:doaj.org-article:a73bdc2102e147b697feb2e838a9014f2021-12-02T16:06:28ZMycoviruses of an endophytic fungus can replicate in plant cells: evolutionary implications10.1038/s41598-017-02017-32045-2322https://doaj.org/article/a73bdc2102e147b697feb2e838a9014f2017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02017-3https://doaj.org/toc/2045-2322Abstract So far there is no record of a specific virus able to infect both fungal and plant hosts in nature. However, experimental evidence shows that some plant virus RdRPs are able to perform replication in trans of genomic or DI RNAs in the yeast Saccharomyces cerevisiae. Furthermore, tobacco mosaic virus was recently shown to replicate in a filamentous ascomycetous fungus. Thus, at least experimentally, some plant viruses can infect some fungi. Endophytic fungi have been reported from many plants and several of these fungi have been shown to contain viruses. Here we tested if mycoviruses derived from a marine plant endophyte can replicate in plant cells. For this purpose, we used partially purified viral particles from isolate MUT4330 of Penicillium aurantiogriseum var. viridicatum which harbors six virus species, some having dsRNA and some positive-strand ssRNA genomes. These were transfected into three distinct plant protoplast cell systems. Time-course analysis of absolute RNA accumulation provided for the first time evidence that viruses of two species belonging to the Partitiviridae and Totiviridae families, can replicate in plant cells without evidence of host adaptation, i.e, changes in their nucleotide sequence.L. NervaG. C. VareseB. W. FalkM. TurinaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q L. Nerva G. C. Varese B. W. Falk M. Turina Mycoviruses of an endophytic fungus can replicate in plant cells: evolutionary implications
description	Abstract So far there is no record of a specific virus able to infect both fungal and plant hosts in nature. However, experimental evidence shows that some plant virus RdRPs are able to perform replication in trans of genomic or DI RNAs in the yeast Saccharomyces cerevisiae. Furthermore, tobacco mosaic virus was recently shown to replicate in a filamentous ascomycetous fungus. Thus, at least experimentally, some plant viruses can infect some fungi. Endophytic fungi have been reported from many plants and several of these fungi have been shown to contain viruses. Here we tested if mycoviruses derived from a marine plant endophyte can replicate in plant cells. For this purpose, we used partially purified viral particles from isolate MUT4330 of Penicillium aurantiogriseum var. viridicatum which harbors six virus species, some having dsRNA and some positive-strand ssRNA genomes. These were transfected into three distinct plant protoplast cell systems. Time-course analysis of absolute RNA accumulation provided for the first time evidence that viruses of two species belonging to the Partitiviridae and Totiviridae families, can replicate in plant cells without evidence of host adaptation, i.e, changes in their nucleotide sequence.
format	article
author	L. Nerva G. C. Varese B. W. Falk M. Turina
author_facet	L. Nerva G. C. Varese B. W. Falk M. Turina
author_sort	L. Nerva
title	Mycoviruses of an endophytic fungus can replicate in plant cells: evolutionary implications
title_short	Mycoviruses of an endophytic fungus can replicate in plant cells: evolutionary implications
title_full	Mycoviruses of an endophytic fungus can replicate in plant cells: evolutionary implications
title_fullStr	Mycoviruses of an endophytic fungus can replicate in plant cells: evolutionary implications
title_full_unstemmed	Mycoviruses of an endophytic fungus can replicate in plant cells: evolutionary implications
title_sort	mycoviruses of an endophytic fungus can replicate in plant cells: evolutionary implications
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/a73bdc2102e147b697feb2e838a9014f
work_keys_str_mv	AT lnerva mycovirusesofanendophyticfunguscanreplicateinplantcellsevolutionaryimplications AT gcvarese mycovirusesofanendophyticfunguscanreplicateinplantcellsevolutionaryimplications AT bwfalk mycovirusesofanendophyticfunguscanreplicateinplantcellsevolutionaryimplications AT mturina mycovirusesofanendophyticfunguscanreplicateinplantcellsevolutionaryimplications
_version_	1718384980929806336

Mycoviruses of an endophytic fungus can replicate in plant cells: evolutionary implications

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