Technical viability of the YF MAC-HD ELISA kit for use in yellow fever-endemic regions.

Yellow fever (YF), an arboviral disease, affects an estimated 200,000 people and causes 30,000 deaths per year and recently has caused major epidemics in Africa and South America. Timely and accurate diagnosis of YF is critical for managing outbreaks and implementing vaccination campaigns. A YF immu...

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Autores principales:	Christin H Goodman, Maurice Demanou, Mick Mulders, Jairo Mendez-Rico, Alison Jane Basile
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/a73cadbd193c46158aee4c7fb0f13253
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spelling	oai:doaj.org-article:a73cadbd193c46158aee4c7fb0f132532021-11-25T06:31:48ZTechnical viability of the YF MAC-HD ELISA kit for use in yellow fever-endemic regions.1935-27271935-273510.1371/journal.pntd.0009417https://doaj.org/article/a73cadbd193c46158aee4c7fb0f132532021-06-01T00:00:00Zhttps://doi.org/10.1371/journal.pntd.0009417https://doaj.org/toc/1935-2727https://doaj.org/toc/1935-2735Yellow fever (YF), an arboviral disease, affects an estimated 200,000 people and causes 30,000 deaths per year and recently has caused major epidemics in Africa and South America. Timely and accurate diagnosis of YF is critical for managing outbreaks and implementing vaccination campaigns. A YF immunoglobulin M (IgM) antibody-capture (MAC) enzyme-linked immunosorbent assay (ELISA) kit, the YF MAC-HD, was successfully introduced starting in 2018 to laboratories in Africa and South America. The YF MAC-HD kit can be performed in 3.5 hours, test up to 24 samples, and includes all reagents necessary to perform the test, except for water used to dilute wash buffer. In 2018 and 2019, a total of 56 laboratory personnel from 39 countries in Africa and South America were trained to use the kit during workshops, followed by take-home YF IgM proficiency testing (PT) exercises. Participants received either a 10- or 20-sample YF PT panel and performed testing using the YF MAC-HD kit. All countries obtained 90% or higher correct results. These results verified the technical viability and transferability of YF MAC-HD kit use for laboratories in YF-endemic countries.Christin H GoodmanMaurice DemanouMick MuldersJairo Mendez-RicoAlison Jane BasilePublic Library of Science (PLoS)articleArctic medicine. Tropical medicineRC955-962Public aspects of medicineRA1-1270ENPLoS Neglected Tropical Diseases, Vol 15, Iss 6, p e0009417 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
spellingShingle	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Christin H Goodman Maurice Demanou Mick Mulders Jairo Mendez-Rico Alison Jane Basile Technical viability of the YF MAC-HD ELISA kit for use in yellow fever-endemic regions.
description	Yellow fever (YF), an arboviral disease, affects an estimated 200,000 people and causes 30,000 deaths per year and recently has caused major epidemics in Africa and South America. Timely and accurate diagnosis of YF is critical for managing outbreaks and implementing vaccination campaigns. A YF immunoglobulin M (IgM) antibody-capture (MAC) enzyme-linked immunosorbent assay (ELISA) kit, the YF MAC-HD, was successfully introduced starting in 2018 to laboratories in Africa and South America. The YF MAC-HD kit can be performed in 3.5 hours, test up to 24 samples, and includes all reagents necessary to perform the test, except for water used to dilute wash buffer. In 2018 and 2019, a total of 56 laboratory personnel from 39 countries in Africa and South America were trained to use the kit during workshops, followed by take-home YF IgM proficiency testing (PT) exercises. Participants received either a 10- or 20-sample YF PT panel and performed testing using the YF MAC-HD kit. All countries obtained 90% or higher correct results. These results verified the technical viability and transferability of YF MAC-HD kit use for laboratories in YF-endemic countries.
format	article
author	Christin H Goodman Maurice Demanou Mick Mulders Jairo Mendez-Rico Alison Jane Basile
author_facet	Christin H Goodman Maurice Demanou Mick Mulders Jairo Mendez-Rico Alison Jane Basile
author_sort	Christin H Goodman
title	Technical viability of the YF MAC-HD ELISA kit for use in yellow fever-endemic regions.
title_short	Technical viability of the YF MAC-HD ELISA kit for use in yellow fever-endemic regions.
title_full	Technical viability of the YF MAC-HD ELISA kit for use in yellow fever-endemic regions.
title_fullStr	Technical viability of the YF MAC-HD ELISA kit for use in yellow fever-endemic regions.
title_full_unstemmed	Technical viability of the YF MAC-HD ELISA kit for use in yellow fever-endemic regions.
title_sort	technical viability of the yf mac-hd elisa kit for use in yellow fever-endemic regions.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/a73cadbd193c46158aee4c7fb0f13253
work_keys_str_mv	AT christinhgoodman technicalviabilityoftheyfmachdelisakitforuseinyellowfeverendemicregions AT mauricedemanou technicalviabilityoftheyfmachdelisakitforuseinyellowfeverendemicregions AT mickmulders technicalviabilityoftheyfmachdelisakitforuseinyellowfeverendemicregions AT jairomendezrico technicalviabilityoftheyfmachdelisakitforuseinyellowfeverendemicregions AT alisonjanebasile technicalviabilityoftheyfmachdelisakitforuseinyellowfeverendemicregions
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Technical viability of the YF MAC-HD ELISA kit for use in yellow fever-endemic regions.

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