Chromosomes in the porcine first polar body possess competence of second meiotic division within enucleated MII stage oocytes.

To determine whether chromosomes in the porcine first polar body (PB1) can complete the second meiotic division and subsequently undergo normal pre-implantation embryonic development, we examined the developmental competence of PB1 chromosomes injected into enucleated MII stage oocytes by nuclear tr...

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Autores principales:	Tao Lin, Yun Fei Diao, Jung Won Kang, Jae Eun Lee, Dong Kyo Kim, Dong Il Jin
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a7437310095742d08c88bed719b47845
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spelling	oai:doaj.org-article:a7437310095742d08c88bed719b478452021-11-18T08:43:41ZChromosomes in the porcine first polar body possess competence of second meiotic division within enucleated MII stage oocytes.1932-620310.1371/journal.pone.0082766https://doaj.org/article/a7437310095742d08c88bed719b478452013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24312673/?tool=EBIhttps://doaj.org/toc/1932-6203To determine whether chromosomes in the porcine first polar body (PB1) can complete the second meiotic division and subsequently undergo normal pre-implantation embryonic development, we examined the developmental competence of PB1 chromosomes injected into enucleated MII stage oocytes by nuclear transfer method (chromosome replacement group, CR group). After parthenogenetic activation (PA) or in vitro fertilization (IVF), the cleavage rate of reconstructed oocytes in the IVF group (CR-IVF group, 36.4 ± 3.2%) and PA group (CR-PA group, 50.8 ± 4.2%) were significantly lower than that of control groups in which normal MII oocytes were subjected to IVF (MII-IVF group, 75.8 ± 1.5%) and PA (MII-PA group, 86.9 ± 3.7%). Unfertilized rates was significantly higher in the CR-IVF group (48.6 ± 3.3%) than in the MII-IVF group (13.1 ± 3.4%). The blastocyst formation rate was 8.3 ± 1.9% in the CR-PA group, whereas no blastocyst formation was observed in the CR-IVF group. To produce tetraploid parthenogenetic embryos, intact MII stage oocytes injected with PB1 chromosomes were electrically stimulated, treated with 7.5 μg/mL cytochalasin B for 3 h (MII oocyte + PB1 + CB group), and then cultured without cytochalasin B. The average cleavage rate of reconstructed oocytes was 72.5% (48 of 66), and the blastocyst formation rate was 18.7% (9 of 48). Chromosome analysis showed similar proportions of haploid and diploid cells in the control (normal MII oocytes) and CR groups after PA; overall, 23.6% of blastocysts were tetraploid in the MII oocyte + PB1 + CB group. These results demonstrate that chromosomes in PB1 can participate in normal pre-implantation embryonic development when injected into enucleated MII stage oocytes, and that tetraploid PA blastocysts are produced (although at a low proportion) when PB1 chromosomes are injected into intact MII stage oocytes.Tao LinYun Fei DiaoJung Won KangJae Eun LeeDong Kyo KimDong Il JinPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 12, p e82766 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tao Lin Yun Fei Diao Jung Won Kang Jae Eun Lee Dong Kyo Kim Dong Il Jin Chromosomes in the porcine first polar body possess competence of second meiotic division within enucleated MII stage oocytes.
description	To determine whether chromosomes in the porcine first polar body (PB1) can complete the second meiotic division and subsequently undergo normal pre-implantation embryonic development, we examined the developmental competence of PB1 chromosomes injected into enucleated MII stage oocytes by nuclear transfer method (chromosome replacement group, CR group). After parthenogenetic activation (PA) or in vitro fertilization (IVF), the cleavage rate of reconstructed oocytes in the IVF group (CR-IVF group, 36.4 ± 3.2%) and PA group (CR-PA group, 50.8 ± 4.2%) were significantly lower than that of control groups in which normal MII oocytes were subjected to IVF (MII-IVF group, 75.8 ± 1.5%) and PA (MII-PA group, 86.9 ± 3.7%). Unfertilized rates was significantly higher in the CR-IVF group (48.6 ± 3.3%) than in the MII-IVF group (13.1 ± 3.4%). The blastocyst formation rate was 8.3 ± 1.9% in the CR-PA group, whereas no blastocyst formation was observed in the CR-IVF group. To produce tetraploid parthenogenetic embryos, intact MII stage oocytes injected with PB1 chromosomes were electrically stimulated, treated with 7.5 μg/mL cytochalasin B for 3 h (MII oocyte + PB1 + CB group), and then cultured without cytochalasin B. The average cleavage rate of reconstructed oocytes was 72.5% (48 of 66), and the blastocyst formation rate was 18.7% (9 of 48). Chromosome analysis showed similar proportions of haploid and diploid cells in the control (normal MII oocytes) and CR groups after PA; overall, 23.6% of blastocysts were tetraploid in the MII oocyte + PB1 + CB group. These results demonstrate that chromosomes in PB1 can participate in normal pre-implantation embryonic development when injected into enucleated MII stage oocytes, and that tetraploid PA blastocysts are produced (although at a low proportion) when PB1 chromosomes are injected into intact MII stage oocytes.
format	article
author	Tao Lin Yun Fei Diao Jung Won Kang Jae Eun Lee Dong Kyo Kim Dong Il Jin
author_facet	Tao Lin Yun Fei Diao Jung Won Kang Jae Eun Lee Dong Kyo Kim Dong Il Jin
author_sort	Tao Lin
title	Chromosomes in the porcine first polar body possess competence of second meiotic division within enucleated MII stage oocytes.
title_short	Chromosomes in the porcine first polar body possess competence of second meiotic division within enucleated MII stage oocytes.
title_full	Chromosomes in the porcine first polar body possess competence of second meiotic division within enucleated MII stage oocytes.
title_fullStr	Chromosomes in the porcine first polar body possess competence of second meiotic division within enucleated MII stage oocytes.
title_full_unstemmed	Chromosomes in the porcine first polar body possess competence of second meiotic division within enucleated MII stage oocytes.
title_sort	chromosomes in the porcine first polar body possess competence of second meiotic division within enucleated mii stage oocytes.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/a7437310095742d08c88bed719b47845
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Chromosomes in the porcine first polar body possess competence of second meiotic division within enucleated MII stage oocytes.

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