Structural and functional analysis of lysozyme after treatment with dielectric barrier discharge plasma and atmospheric pressure plasma jet

Abstract The variation in the biological function of proteins plays an important role in plasma medicine and sterilization. Several non-thermal plasma sources with different feeding gases are used worldwide for plasma treatment, including dielectric barrier discharge (DBD) and atmospheric-pressure p...

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Autores principales:	Sooho Choi, Pankaj Attri, Inhwan Lee, Jeongmin Oh, Ji-Hye Yun, Ji Hoon Park, Eun Ha Choi, Weontae Lee
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a7451f199dfe4bf88dddf1c755422c12
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spelling	oai:doaj.org-article:a7451f199dfe4bf88dddf1c755422c122021-12-02T15:05:28ZStructural and functional analysis of lysozyme after treatment with dielectric barrier discharge plasma and atmospheric pressure plasma jet10.1038/s41598-017-01030-w2045-2322https://doaj.org/article/a7451f199dfe4bf88dddf1c755422c122017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01030-whttps://doaj.org/toc/2045-2322Abstract The variation in the biological function of proteins plays an important role in plasma medicine and sterilization. Several non-thermal plasma sources with different feeding gases are used worldwide for plasma treatment, including dielectric barrier discharge (DBD) and atmospheric-pressure plasma jet (APPJ) as the most commonly used sources. Therefore, in the present work, we used both DBD and APPJ plasma sources with N2 and air as feeding gases to evaluate the effects on the structural, thermodynamic, and activity changes of enzymes. In the current work, we used lysozyme as a model enzyme and verified the structural changes using circular dichroism (CD), fluorescence, and X-ray crystallography. In addition, we investigated the lysozyme thermodynamics using CD thermal analysis and changes in the B-factor from X-ray crystallography. The results showed that lysozyme activity decreased after the plasma treatment. From these analyses, we concluded that N2-feeding gas plasma disturbs the structure and activity of lysozyme more than Air feeding gas plasma in our experimental studies. This study provides novel fundamental information on the changes to enzymes upon plasma treatment, which has been absent from the literature until now.Sooho ChoiPankaj AttriInhwan LeeJeongmin OhJi-Hye YunJi Hoon ParkEun Ha ChoiWeontae LeeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sooho Choi Pankaj Attri Inhwan Lee Jeongmin Oh Ji-Hye Yun Ji Hoon Park Eun Ha Choi Weontae Lee Structural and functional analysis of lysozyme after treatment with dielectric barrier discharge plasma and atmospheric pressure plasma jet
description	Abstract The variation in the biological function of proteins plays an important role in plasma medicine and sterilization. Several non-thermal plasma sources with different feeding gases are used worldwide for plasma treatment, including dielectric barrier discharge (DBD) and atmospheric-pressure plasma jet (APPJ) as the most commonly used sources. Therefore, in the present work, we used both DBD and APPJ plasma sources with N2 and air as feeding gases to evaluate the effects on the structural, thermodynamic, and activity changes of enzymes. In the current work, we used lysozyme as a model enzyme and verified the structural changes using circular dichroism (CD), fluorescence, and X-ray crystallography. In addition, we investigated the lysozyme thermodynamics using CD thermal analysis and changes in the B-factor from X-ray crystallography. The results showed that lysozyme activity decreased after the plasma treatment. From these analyses, we concluded that N2-feeding gas plasma disturbs the structure and activity of lysozyme more than Air feeding gas plasma in our experimental studies. This study provides novel fundamental information on the changes to enzymes upon plasma treatment, which has been absent from the literature until now.
format	article
author	Sooho Choi Pankaj Attri Inhwan Lee Jeongmin Oh Ji-Hye Yun Ji Hoon Park Eun Ha Choi Weontae Lee
author_facet	Sooho Choi Pankaj Attri Inhwan Lee Jeongmin Oh Ji-Hye Yun Ji Hoon Park Eun Ha Choi Weontae Lee
author_sort	Sooho Choi
title	Structural and functional analysis of lysozyme after treatment with dielectric barrier discharge plasma and atmospheric pressure plasma jet
title_short	Structural and functional analysis of lysozyme after treatment with dielectric barrier discharge plasma and atmospheric pressure plasma jet
title_full	Structural and functional analysis of lysozyme after treatment with dielectric barrier discharge plasma and atmospheric pressure plasma jet
title_fullStr	Structural and functional analysis of lysozyme after treatment with dielectric barrier discharge plasma and atmospheric pressure plasma jet
title_full_unstemmed	Structural and functional analysis of lysozyme after treatment with dielectric barrier discharge plasma and atmospheric pressure plasma jet
title_sort	structural and functional analysis of lysozyme after treatment with dielectric barrier discharge plasma and atmospheric pressure plasma jet
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/a7451f199dfe4bf88dddf1c755422c12
work_keys_str_mv	AT soohochoi structuralandfunctionalanalysisoflysozymeaftertreatmentwithdielectricbarrierdischargeplasmaandatmosphericpressureplasmajet AT pankajattri structuralandfunctionalanalysisoflysozymeaftertreatmentwithdielectricbarrierdischargeplasmaandatmosphericpressureplasmajet AT inhwanlee structuralandfunctionalanalysisoflysozymeaftertreatmentwithdielectricbarrierdischargeplasmaandatmosphericpressureplasmajet AT jeongminoh structuralandfunctionalanalysisoflysozymeaftertreatmentwithdielectricbarrierdischargeplasmaandatmosphericpressureplasmajet AT jihyeyun structuralandfunctionalanalysisoflysozymeaftertreatmentwithdielectricbarrierdischargeplasmaandatmosphericpressureplasmajet AT jihoonpark structuralandfunctionalanalysisoflysozymeaftertreatmentwithdielectricbarrierdischargeplasmaandatmosphericpressureplasmajet AT eunhachoi structuralandfunctionalanalysisoflysozymeaftertreatmentwithdielectricbarrierdischargeplasmaandatmosphericpressureplasmajet AT weontaelee structuralandfunctionalanalysisoflysozymeaftertreatmentwithdielectricbarrierdischargeplasmaandatmosphericpressureplasmajet
_version_	1718388850832703488

Structural and functional analysis of lysozyme after treatment with dielectric barrier discharge plasma and atmospheric pressure plasma jet

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