Comprehensive coverage of human last meal components revealed by a forensic DNA metabarcoding approach

Abstract Stomach content analyses are a valuable tool in human forensic science to interpret perimortem events. While the identification of food components of plant and animal origin has traditionally been conducted by macro- and microscopical approaches in case of incomplete digestion, molecular me...

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Autores principales:	Judith Schneider, Eduard Mas-Carrió, Catherine Jan, Christian Miquel, Pierre Taberlet, Katarzyna Michaud, Luca Fumagalli
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a7464cb269f54e61bab83683f116b3e9
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spelling	oai:doaj.org-article:a7464cb269f54e61bab83683f116b3e92021-12-02T17:32:57ZComprehensive coverage of human last meal components revealed by a forensic DNA metabarcoding approach10.1038/s41598-021-88418-x2045-2322https://doaj.org/article/a7464cb269f54e61bab83683f116b3e92021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88418-xhttps://doaj.org/toc/2045-2322Abstract Stomach content analyses are a valuable tool in human forensic science to interpret perimortem events. While the identification of food components of plant and animal origin has traditionally been conducted by macro- and microscopical approaches in case of incomplete digestion, molecular methods provide the potential to increase sensitivity and taxonomic resolution. In particular, DNA metabarcoding (PCR-amplification and next generation sequencing of complex DNA mixtures) has seen a rapid growth in the field of wildlife ecology to assess species’ diets from faecal and gastric samples. Despite clear advantages, molecular approaches have not yet been established in routine human forensics to investigate the last meal components of deceased persons. In this pilot study we applied for the first time a DNA metabarcoding approach to assess both plant and vertebrate components of 48 human stomach content samples taken during medicolegal autopsies. We obtained a final dataset with 34 vertebrate and 124 vegetal unique sequences, that were clustered to 9 and 33 operational taxonomic units (OTUs), respectively. Our results suggest that this approach can provide crucial information about circumstances preceding death, and open promising perspectives for biomedical dietary surveys based on digested food items found in the gastrointestinal tract.Judith SchneiderEduard Mas-CarrióCatherine JanChristian MiquelPierre TaberletKatarzyna MichaudLuca FumagalliNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Judith Schneider Eduard Mas-Carrió Catherine Jan Christian Miquel Pierre Taberlet Katarzyna Michaud Luca Fumagalli Comprehensive coverage of human last meal components revealed by a forensic DNA metabarcoding approach
description	Abstract Stomach content analyses are a valuable tool in human forensic science to interpret perimortem events. While the identification of food components of plant and animal origin has traditionally been conducted by macro- and microscopical approaches in case of incomplete digestion, molecular methods provide the potential to increase sensitivity and taxonomic resolution. In particular, DNA metabarcoding (PCR-amplification and next generation sequencing of complex DNA mixtures) has seen a rapid growth in the field of wildlife ecology to assess species’ diets from faecal and gastric samples. Despite clear advantages, molecular approaches have not yet been established in routine human forensics to investigate the last meal components of deceased persons. In this pilot study we applied for the first time a DNA metabarcoding approach to assess both plant and vertebrate components of 48 human stomach content samples taken during medicolegal autopsies. We obtained a final dataset with 34 vertebrate and 124 vegetal unique sequences, that were clustered to 9 and 33 operational taxonomic units (OTUs), respectively. Our results suggest that this approach can provide crucial information about circumstances preceding death, and open promising perspectives for biomedical dietary surveys based on digested food items found in the gastrointestinal tract.
format	article
author	Judith Schneider Eduard Mas-Carrió Catherine Jan Christian Miquel Pierre Taberlet Katarzyna Michaud Luca Fumagalli
author_facet	Judith Schneider Eduard Mas-Carrió Catherine Jan Christian Miquel Pierre Taberlet Katarzyna Michaud Luca Fumagalli
author_sort	Judith Schneider
title	Comprehensive coverage of human last meal components revealed by a forensic DNA metabarcoding approach
title_short	Comprehensive coverage of human last meal components revealed by a forensic DNA metabarcoding approach
title_full	Comprehensive coverage of human last meal components revealed by a forensic DNA metabarcoding approach
title_fullStr	Comprehensive coverage of human last meal components revealed by a forensic DNA metabarcoding approach
title_full_unstemmed	Comprehensive coverage of human last meal components revealed by a forensic DNA metabarcoding approach
title_sort	comprehensive coverage of human last meal components revealed by a forensic dna metabarcoding approach
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/a7464cb269f54e61bab83683f116b3e9
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Comprehensive coverage of human last meal components revealed by a forensic DNA metabarcoding approach

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