The Mechanically Extraction Process of Gambier (Uncaria gambier Roxb.) from Limapuluh Kota, West Sumatera and Its Antioxidant activity

The extraction process from leaves and twigs of gambier (Uncaria gambier Roxb)plant was conducted mechanically by using traditional hydraulic press, conventional screw press, and modified twin-screw press. The leaves and twigs of gambier plant was obtained from traditional farmer in Limapuluh Kota,...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Galuh Widiyarti, Andini Sundowo, Euis Filailla, Joddy Arya Laksmono
Formato:	article
Lenguaje:	EN
Publicado:	University of Brawijaya 2020
Materias:	gambier mechanically extraction antioxidant dpph Chemistry QD1-999
Acceso en línea:	http://dx.doi.org/10.21776/jpacr.ub.2020.009.01.509 https://doaj.org/article/a747bc7322044b95aa963923a4446a62
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a747bc7322044b95aa963923a4446a62
record_format	dspace
spelling	oai:doaj.org-article:a747bc7322044b95aa963923a4446a622021-12-02T14:22:18ZThe Mechanically Extraction Process of Gambier (Uncaria gambier Roxb.) from Limapuluh Kota, West Sumatera and Its Antioxidant activityhttp://dx.doi.org/10.21776/jpacr.ub.2020.009.01.5092302-46902302-4690https://doaj.org/article/a747bc7322044b95aa963923a4446a622020-03-01T00:00:00Zhttps://jpacr.ub.ac.id/index.php/jpacr/article/view/509/pdfhttps://doaj.org/toc/2302-4690https://doaj.org/toc/2302-4690The extraction process from leaves and twigs of gambier (Uncaria gambier Roxb)plant was conducted mechanically by using traditional hydraulic press, conventional screw press, and modified twin-screw press. The leaves and twigs of gambier plant was obtained from traditional farmer in Limapuluh Kota, West Sumatera, Indonesia. The water, ash and catechin contents of the gambier extracts were analyzed based on SNI 01-3391-2000 using spectrophotometry and thermogravimetry method. Antioxidant activity analysis of the extracts was also performed by 1,1-diphenyl-2-picrylhidrazyl (DPPH) method and compared tovitamin C as a standard antioxidant. The analysis results showed that the extracts contain no ash. Meanwhile, the catechin and water contents of the extracts were approximately 50 and 13% thus the extracts were classified as quality gambier 2. Other than that, analysis result of catechin and epicatechin contents of the extracts using HPLC and compared to the reference materials showed that catechins contents of the extracts using traditional hydraulic press, conventionalscrew press, and modified twin-screw press give catechin content in about 94.296-95.030%. However, epicatechin was detected in a trace amount. The antioxidant activity of the extracts were 2.5 times stronger than reference. The IC50 value of 4.37-4.52 µg/mL and was categorized as active antioxidant.Galuh WidiyartiAndini SundowoEuis Filailla Joddy Arya LaksmonoUniversity of Brawijayaarticlegambiermechanically extractionantioxidantdpphChemistryQD1-999ENJournal of Pure and Applied Chemistry Research, Vol 9, Iss 1, Pp 8-15 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	gambier mechanically extraction antioxidant dpph Chemistry QD1-999
spellingShingle	gambier mechanically extraction antioxidant dpph Chemistry QD1-999 Galuh Widiyarti Andini Sundowo Euis Filailla Joddy Arya Laksmono The Mechanically Extraction Process of Gambier (Uncaria gambier Roxb.) from Limapuluh Kota, West Sumatera and Its Antioxidant activity
description	The extraction process from leaves and twigs of gambier (Uncaria gambier Roxb)plant was conducted mechanically by using traditional hydraulic press, conventional screw press, and modified twin-screw press. The leaves and twigs of gambier plant was obtained from traditional farmer in Limapuluh Kota, West Sumatera, Indonesia. The water, ash and catechin contents of the gambier extracts were analyzed based on SNI 01-3391-2000 using spectrophotometry and thermogravimetry method. Antioxidant activity analysis of the extracts was also performed by 1,1-diphenyl-2-picrylhidrazyl (DPPH) method and compared tovitamin C as a standard antioxidant. The analysis results showed that the extracts contain no ash. Meanwhile, the catechin and water contents of the extracts were approximately 50 and 13% thus the extracts were classified as quality gambier 2. Other than that, analysis result of catechin and epicatechin contents of the extracts using HPLC and compared to the reference materials showed that catechins contents of the extracts using traditional hydraulic press, conventionalscrew press, and modified twin-screw press give catechin content in about 94.296-95.030%. However, epicatechin was detected in a trace amount. The antioxidant activity of the extracts were 2.5 times stronger than reference. The IC50 value of 4.37-4.52 µg/mL and was categorized as active antioxidant.
format	article
author	Galuh Widiyarti Andini Sundowo Euis Filailla Joddy Arya Laksmono
author_facet	Galuh Widiyarti Andini Sundowo Euis Filailla Joddy Arya Laksmono
author_sort	Galuh Widiyarti
title	The Mechanically Extraction Process of Gambier (Uncaria gambier Roxb.) from Limapuluh Kota, West Sumatera and Its Antioxidant activity
title_short	The Mechanically Extraction Process of Gambier (Uncaria gambier Roxb.) from Limapuluh Kota, West Sumatera and Its Antioxidant activity
title_full	The Mechanically Extraction Process of Gambier (Uncaria gambier Roxb.) from Limapuluh Kota, West Sumatera and Its Antioxidant activity
title_fullStr	The Mechanically Extraction Process of Gambier (Uncaria gambier Roxb.) from Limapuluh Kota, West Sumatera and Its Antioxidant activity
title_full_unstemmed	The Mechanically Extraction Process of Gambier (Uncaria gambier Roxb.) from Limapuluh Kota, West Sumatera and Its Antioxidant activity
title_sort	mechanically extraction process of gambier (uncaria gambier roxb.) from limapuluh kota, west sumatera and its antioxidant activity
publisher	University of Brawijaya
publishDate	2020
url	http://dx.doi.org/10.21776/jpacr.ub.2020.009.01.509 https://doaj.org/article/a747bc7322044b95aa963923a4446a62
work_keys_str_mv	AT galuhwidiyarti themechanicallyextractionprocessofgambieruncariagambierroxbfromlimapuluhkotawestsumateraanditsantioxidantactivity AT andinisundowo themechanicallyextractionprocessofgambieruncariagambierroxbfromlimapuluhkotawestsumateraanditsantioxidantactivity AT euisfilailla themechanicallyextractionprocessofgambieruncariagambierroxbfromlimapuluhkotawestsumateraanditsantioxidantactivity AT joddyaryalaksmono themechanicallyextractionprocessofgambieruncariagambierroxbfromlimapuluhkotawestsumateraanditsantioxidantactivity AT galuhwidiyarti mechanicallyextractionprocessofgambieruncariagambierroxbfromlimapuluhkotawestsumateraanditsantioxidantactivity AT andinisundowo mechanicallyextractionprocessofgambieruncariagambierroxbfromlimapuluhkotawestsumateraanditsantioxidantactivity AT euisfilailla mechanicallyextractionprocessofgambieruncariagambierroxbfromlimapuluhkotawestsumateraanditsantioxidantactivity AT joddyaryalaksmono mechanicallyextractionprocessofgambieruncariagambierroxbfromlimapuluhkotawestsumateraanditsantioxidantactivity
_version_	1718391507871858688

The Mechanically Extraction Process of Gambier (Uncaria gambier Roxb.) from Limapuluh Kota, West Sumatera and Its Antioxidant activity

Ejemplares similares