Characterization of a newly isolated phage infecting pathogenic Escherichia coli and analysis of its mosaic structural genes

Abstract The outbreak of multidrug-resistant pathogenic bacteria made the discovery of novel control strategies necessary. Phages have regained attention for their specific lytic activity against pathogenic bacterium. A newly isolated phage infecting the clinical Escherichia coli isolates, including...

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Autores principales:	Qin Peng, Yihui Yuan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a74a963640e44195becef8068774ebff
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spelling	oai:doaj.org-article:a74a963640e44195becef8068774ebff2021-12-02T11:41:13ZCharacterization of a newly isolated phage infecting pathogenic Escherichia coli and analysis of its mosaic structural genes10.1038/s41598-018-26004-42045-2322https://doaj.org/article/a74a963640e44195becef8068774ebff2018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-26004-4https://doaj.org/toc/2045-2322Abstract The outbreak of multidrug-resistant pathogenic bacteria made the discovery of novel control strategies necessary. Phages have regained attention for their specific lytic activity against pathogenic bacterium. A newly isolated phage infecting the clinical Escherichia coli isolates, including several multidrug-resistant strains, was isolated, and this phage showed high control effects against the tested pathogenic E. coli strains. Host range analysis revealed that although the phage exhibited broad lytic spectrum against the tested E. coli strains, it could not lyse strains from the other species. Comparative genomic analysis showed that phages had undergone at least three genome recombination events during the evolutionary process at the position of the three phage tail genes, which was reported to be associated with the host range determination of the phage. The recombinant tail proteins contained functional domains that were highly similar with genes of the Salmonella phage and genes of Pseudomonas and Neisseria. The findings of this study not only provide resources for developing phage therapy against E. coli, but also showed the highly variable genome structure of the phage.Qin PengYihui YuanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Qin Peng Yihui Yuan Characterization of a newly isolated phage infecting pathogenic Escherichia coli and analysis of its mosaic structural genes
description	Abstract The outbreak of multidrug-resistant pathogenic bacteria made the discovery of novel control strategies necessary. Phages have regained attention for their specific lytic activity against pathogenic bacterium. A newly isolated phage infecting the clinical Escherichia coli isolates, including several multidrug-resistant strains, was isolated, and this phage showed high control effects against the tested pathogenic E. coli strains. Host range analysis revealed that although the phage exhibited broad lytic spectrum against the tested E. coli strains, it could not lyse strains from the other species. Comparative genomic analysis showed that phages had undergone at least three genome recombination events during the evolutionary process at the position of the three phage tail genes, which was reported to be associated with the host range determination of the phage. The recombinant tail proteins contained functional domains that were highly similar with genes of the Salmonella phage and genes of Pseudomonas and Neisseria. The findings of this study not only provide resources for developing phage therapy against E. coli, but also showed the highly variable genome structure of the phage.
format	article
author	Qin Peng Yihui Yuan
author_facet	Qin Peng Yihui Yuan
author_sort	Qin Peng
title	Characterization of a newly isolated phage infecting pathogenic Escherichia coli and analysis of its mosaic structural genes
title_short	Characterization of a newly isolated phage infecting pathogenic Escherichia coli and analysis of its mosaic structural genes
title_full	Characterization of a newly isolated phage infecting pathogenic Escherichia coli and analysis of its mosaic structural genes
title_fullStr	Characterization of a newly isolated phage infecting pathogenic Escherichia coli and analysis of its mosaic structural genes
title_full_unstemmed	Characterization of a newly isolated phage infecting pathogenic Escherichia coli and analysis of its mosaic structural genes
title_sort	characterization of a newly isolated phage infecting pathogenic escherichia coli and analysis of its mosaic structural genes
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/a74a963640e44195becef8068774ebff
work_keys_str_mv	AT qinpeng characterizationofanewlyisolatedphageinfectingpathogenicescherichiacoliandanalysisofitsmosaicstructuralgenes AT yihuiyuan characterizationofanewlyisolatedphageinfectingpathogenicescherichiacoliandanalysisofitsmosaicstructuralgenes
_version_	1718395417252593664

Characterization of a newly isolated phage infecting pathogenic Escherichia coli and analysis of its mosaic structural genes

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