Proliferation And Differentiation Potential Of Canine Synovial Fluid Cells

The aim of this study was to determine whether synovial fluid (SF) of dogs contains cells that have characteristics of MSCs and to describe their differentiation potential. SF adherent cells from 5 young German shepherd dogs (average 3.8 ± 0.9 years) were expanded (37°C, 5% CO2, humidified atmospher...

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Autores principales:	Jelena FRANCUSKI V, Jasmina DEBELJAK MARTAČIĆ, Anita RADOVANOVIĆ, Nenad ANDRIĆ, Sophie SOURICE-PETIT, Jerome GUICHEUX, Slavko MOJSILOVIĆ, Milica KOVAČEVIĆ FILIPOVIĆ
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2015
Materias:	chondrogenesis dog mesenchymal stem cell synovial fluid Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/a754f79cd47a46e092d82d8f716f27fa
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spelling	oai:doaj.org-article:a754f79cd47a46e092d82d8f716f27fa2021-11-17T21:27:50ZProliferation And Differentiation Potential Of Canine Synovial Fluid Cells1820-744810.1515/acve-2015-0005https://doaj.org/article/a754f79cd47a46e092d82d8f716f27fa2015-03-01T00:00:00Zhttps://doi.org/10.1515/acve-2015-0005https://doaj.org/toc/1820-7448The aim of this study was to determine whether synovial fluid (SF) of dogs contains cells that have characteristics of MSCs and to describe their differentiation potential. SF adherent cells from 5 young German shepherd dogs (average 3.8 ± 0.9 years) were expanded (37°C, 5% CO2, humidified atmosphere) three weeks before their phenotype was characterized by flow-cytometry for the presence of CD90 and CD34. Population doubling time (PDT), number of CFU-F and adipogenic, osteogenic and chondrogenic potentials have been determined in vitro. In early passages PTD was 31 ± 10 hours and expansion fold after 3 sub cultivations (9 days) theoretically could be 372 ± 134. At P1, 0.55 ± 0.05% of SF cells had the ability to form CFU-F. Sixty-six percent of cells expressed CD90 and none of the cells expressed markers of hematopoietic cells. Oil Red O staining has shown accumulation of fat droplets in cells grown in adipogenic medium, while deposits of calcium in the osteogenic medium were evidenced with Alizarin red staining. SF cultured in hondrogenic and control medium in three-dimensional conditions formed a cartilage-like tissue. Alcian blue staining of pellets’ slides have shown a significant amount of glycosaminoglycans (GAGs) and immunohistochemistry analysis documented collagen type II expression. The amount of GAGs in pellets grown in both conditions showed no difference. SF cells in vitro exhibited osteogenic, adipogenic and chondrogenic differentiation potentials, suggesting the presence of different mesenchymal progenitors. These results also demonstrated that SF cells have a spontaneous chondrogenic potential that should be further explored for possible tissue engineering protocols.Jelena FRANCUSKI VJasmina DEBELJAK MARTAČIĆAnita RADOVANOVIĆNenad ANDRIĆSophie SOURICE-PETITJerome GUICHEUXSlavko MOJSILOVIĆMilica KOVAČEVIĆ FILIPOVIĆSciendoarticlechondrogenesisdogmesenchymal stem cellsynovial fluidVeterinary medicineSF600-1100ENActa Veterinaria, Vol 65, Iss 1, Pp 66-78 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	chondrogenesis dog mesenchymal stem cell synovial fluid Veterinary medicine SF600-1100
spellingShingle	chondrogenesis dog mesenchymal stem cell synovial fluid Veterinary medicine SF600-1100 Jelena FRANCUSKI V Jasmina DEBELJAK MARTAČIĆ Anita RADOVANOVIĆ Nenad ANDRIĆ Sophie SOURICE-PETIT Jerome GUICHEUX Slavko MOJSILOVIĆ Milica KOVAČEVIĆ FILIPOVIĆ Proliferation And Differentiation Potential Of Canine Synovial Fluid Cells
description	The aim of this study was to determine whether synovial fluid (SF) of dogs contains cells that have characteristics of MSCs and to describe their differentiation potential. SF adherent cells from 5 young German shepherd dogs (average 3.8 ± 0.9 years) were expanded (37°C, 5% CO2, humidified atmosphere) three weeks before their phenotype was characterized by flow-cytometry for the presence of CD90 and CD34. Population doubling time (PDT), number of CFU-F and adipogenic, osteogenic and chondrogenic potentials have been determined in vitro. In early passages PTD was 31 ± 10 hours and expansion fold after 3 sub cultivations (9 days) theoretically could be 372 ± 134. At P1, 0.55 ± 0.05% of SF cells had the ability to form CFU-F. Sixty-six percent of cells expressed CD90 and none of the cells expressed markers of hematopoietic cells. Oil Red O staining has shown accumulation of fat droplets in cells grown in adipogenic medium, while deposits of calcium in the osteogenic medium were evidenced with Alizarin red staining. SF cultured in hondrogenic and control medium in three-dimensional conditions formed a cartilage-like tissue. Alcian blue staining of pellets’ slides have shown a significant amount of glycosaminoglycans (GAGs) and immunohistochemistry analysis documented collagen type II expression. The amount of GAGs in pellets grown in both conditions showed no difference. SF cells in vitro exhibited osteogenic, adipogenic and chondrogenic differentiation potentials, suggesting the presence of different mesenchymal progenitors. These results also demonstrated that SF cells have a spontaneous chondrogenic potential that should be further explored for possible tissue engineering protocols.
format	article
author	Jelena FRANCUSKI V Jasmina DEBELJAK MARTAČIĆ Anita RADOVANOVIĆ Nenad ANDRIĆ Sophie SOURICE-PETIT Jerome GUICHEUX Slavko MOJSILOVIĆ Milica KOVAČEVIĆ FILIPOVIĆ
author_facet	Jelena FRANCUSKI V Jasmina DEBELJAK MARTAČIĆ Anita RADOVANOVIĆ Nenad ANDRIĆ Sophie SOURICE-PETIT Jerome GUICHEUX Slavko MOJSILOVIĆ Milica KOVAČEVIĆ FILIPOVIĆ
author_sort	Jelena FRANCUSKI V
title	Proliferation And Differentiation Potential Of Canine Synovial Fluid Cells
title_short	Proliferation And Differentiation Potential Of Canine Synovial Fluid Cells
title_full	Proliferation And Differentiation Potential Of Canine Synovial Fluid Cells
title_fullStr	Proliferation And Differentiation Potential Of Canine Synovial Fluid Cells
title_full_unstemmed	Proliferation And Differentiation Potential Of Canine Synovial Fluid Cells
title_sort	proliferation and differentiation potential of canine synovial fluid cells
publisher	Sciendo
publishDate	2015
url	https://doaj.org/article/a754f79cd47a46e092d82d8f716f27fa
work_keys_str_mv	AT jelenafrancuskiv proliferationanddifferentiationpotentialofcaninesynovialfluidcells AT jasminadebeljakmartacic proliferationanddifferentiationpotentialofcaninesynovialfluidcells AT anitaradovanovic proliferationanddifferentiationpotentialofcaninesynovialfluidcells AT nenadandric proliferationanddifferentiationpotentialofcaninesynovialfluidcells AT sophiesouricepetit proliferationanddifferentiationpotentialofcaninesynovialfluidcells AT jeromeguicheux proliferationanddifferentiationpotentialofcaninesynovialfluidcells AT slavkomojsilovic proliferationanddifferentiationpotentialofcaninesynovialfluidcells AT milicakovacevicfilipovic proliferationanddifferentiationpotentialofcaninesynovialfluidcells
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Proliferation And Differentiation Potential Of Canine Synovial Fluid Cells

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