Intracellular delivery of doxorubicin encapsulated in novel pH-responsive chitosan/heparin nanocapsules

Midhun B Thomas,1,* Krishna Radhakrishnan,1,* Divya P Gnanadhas,2,* Dipshikha Chakravortty,2 Ashok M Raichur1,31Department of Materials Engineering, 2Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, India; 3Department of Applied Chemistry, University of Johannesbu...

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Autores principales:	Thomas MB, Radhakrishnan K, Gnanadhas DP, Chakravortty D, Raichur AM
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/a758bc4b0d0c4d7cbb456a73f5bcdbae
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spelling	oai:doaj.org-article:a758bc4b0d0c4d7cbb456a73f5bcdbae2021-12-02T04:54:04ZIntracellular delivery of doxorubicin encapsulated in novel pH-responsive chitosan/heparin nanocapsules1176-91141178-2013https://doaj.org/article/a758bc4b0d0c4d7cbb456a73f5bcdbae2013-01-01T00:00:00Zhttp://www.dovepress.com/intracellular-delivery-of-doxorubicin-encapsulated-in-novel-ph-respons-a11940https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Midhun B Thomas,1,* Krishna Radhakrishnan,1,* Divya P Gnanadhas,2,* Dipshikha Chakravortty,2 Ashok M Raichur1,31Department of Materials Engineering, 2Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, India; 3Department of Applied Chemistry, University of Johannesburg, Doornfontein, South Africa*These authors contributed equally to this workAbstract: A novel polyelectrolyte nanocapsule system composed of biopolymers, chitosan and heparin has been fabricated by the layer-by-layer technique on silica nanoparticles followed by dissolution of the silica core. The nanocapsules were of the size range 200 &plusmn; 20 nm and loaded with the positively charged anticancer drug doxorubicin with an efficiency of 89%. The loading of the drug into the capsule happens by virtue of the pH-responsive property of the capsule wall, which is determined by the pKa of the polyelectrolytes. As the pH is varied, about 64% of the drug is released in acidic pH while 77% is released in neutral pH. The biocompatibility, efficiency of drug loading, and enhanced bioavailability of the capsule system was confirmed by MTT assay and in vivo biodistribution studies.Keywords: drug delivery, layer-by-layer, electrostatic interaction, biocompatibleThomas MBRadhakrishnan KGnanadhas DPChakravortty DRaichur AMDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2013, Iss default, Pp 267-273 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Thomas MB Radhakrishnan K Gnanadhas DP Chakravortty D Raichur AM Intracellular delivery of doxorubicin encapsulated in novel pH-responsive chitosan/heparin nanocapsules
description	Midhun B Thomas,1,* Krishna Radhakrishnan,1,* Divya P Gnanadhas,2,* Dipshikha Chakravortty,2 Ashok M Raichur1,31Department of Materials Engineering, 2Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, India; 3Department of Applied Chemistry, University of Johannesburg, Doornfontein, South Africa*These authors contributed equally to this workAbstract: A novel polyelectrolyte nanocapsule system composed of biopolymers, chitosan and heparin has been fabricated by the layer-by-layer technique on silica nanoparticles followed by dissolution of the silica core. The nanocapsules were of the size range 200 &plusmn; 20 nm and loaded with the positively charged anticancer drug doxorubicin with an efficiency of 89%. The loading of the drug into the capsule happens by virtue of the pH-responsive property of the capsule wall, which is determined by the pKa of the polyelectrolytes. As the pH is varied, about 64% of the drug is released in acidic pH while 77% is released in neutral pH. The biocompatibility, efficiency of drug loading, and enhanced bioavailability of the capsule system was confirmed by MTT assay and in vivo biodistribution studies.Keywords: drug delivery, layer-by-layer, electrostatic interaction, biocompatible
format	article
author	Thomas MB Radhakrishnan K Gnanadhas DP Chakravortty D Raichur AM
author_facet	Thomas MB Radhakrishnan K Gnanadhas DP Chakravortty D Raichur AM
author_sort	Thomas MB
title	Intracellular delivery of doxorubicin encapsulated in novel pH-responsive chitosan/heparin nanocapsules
title_short	Intracellular delivery of doxorubicin encapsulated in novel pH-responsive chitosan/heparin nanocapsules
title_full	Intracellular delivery of doxorubicin encapsulated in novel pH-responsive chitosan/heparin nanocapsules
title_fullStr	Intracellular delivery of doxorubicin encapsulated in novel pH-responsive chitosan/heparin nanocapsules
title_full_unstemmed	Intracellular delivery of doxorubicin encapsulated in novel pH-responsive chitosan/heparin nanocapsules
title_sort	intracellular delivery of doxorubicin encapsulated in novel ph-responsive chitosan/heparin nanocapsules
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/a758bc4b0d0c4d7cbb456a73f5bcdbae
work_keys_str_mv	AT thomasmb intracellulardeliveryofdoxorubicinencapsulatedinnovelphresponsivechitosanheparinnanocapsules AT radhakrishnank intracellulardeliveryofdoxorubicinencapsulatedinnovelphresponsivechitosanheparinnanocapsules AT gnanadhasdp intracellulardeliveryofdoxorubicinencapsulatedinnovelphresponsivechitosanheparinnanocapsules AT chakravorttyd intracellulardeliveryofdoxorubicinencapsulatedinnovelphresponsivechitosanheparinnanocapsules AT raichuram intracellulardeliveryofdoxorubicinencapsulatedinnovelphresponsivechitosanheparinnanocapsules
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Intracellular delivery of doxorubicin encapsulated in novel pH-responsive chitosan/heparin nanocapsules

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