Fate of transgenic DNA from orally administered Bt MON810 maize and effects on immune response and growth in pigs.

We assessed the effect of short-term feeding of genetically modified (GM: Bt MON810) maize on immune responses and growth in weanling pigs and determined the fate of the transgenic DNA and protein in-vivo. Pigs were fed a diet containing 38.9% GM or non-GM isogenic parent line maize for 31 days. We...

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Autores principales:	Maria C Walsh, Stefan G Buzoianu, Gillian E Gardiner, Mary C Rea, Eva Gelencsér, Anna Jánosi, Michelle M Epstein, R Paul Ross, Peadar G Lawlor
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a75b611a11ac46efb2c41b30f9f16a80
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spelling	oai:doaj.org-article:a75b611a11ac46efb2c41b30f9f16a802021-11-18T07:33:41ZFate of transgenic DNA from orally administered Bt MON810 maize and effects on immune response and growth in pigs.1932-620310.1371/journal.pone.0027177https://doaj.org/article/a75b611a11ac46efb2c41b30f9f16a802011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22132091/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203We assessed the effect of short-term feeding of genetically modified (GM: Bt MON810) maize on immune responses and growth in weanling pigs and determined the fate of the transgenic DNA and protein in-vivo. Pigs were fed a diet containing 38.9% GM or non-GM isogenic parent line maize for 31 days. We observed that IL-12 and IFNγ production from mitogenic stimulated peripheral blood mononuclear cells decreased (P<0.10) following 31 days of GM maize exposure. While Cry1Ab-specific IgG and IgA were not detected in the plasma of GM maize-fed pigs, the detection of the cry1Ab gene and protein was limited to the gastrointestinal digesta and was not found in the kidneys, liver, spleen, muscle, heart or blood. Feeding GM maize to weanling pigs had no effect on growth performance or body weight. IL-6 and IL-4 production from isolated splenocytes were increased (P<0.05) in response to feeding GM maize while the proportion of CD4(+) T cells in the spleen decreased. In the ileum, the proportion of B cells and macrophages decreased while the proportion of CD4(+) T cells increased in GM maize-fed pigs. IL-8 and IL-4 production from isolated intraepithelial and lamina propria lymphocytes were also increased (P<0.05) in response to feeding GM maize. In conclusion, there was no evidence of cry1Ab gene or protein translocation to the organs and blood of weaning pigs. The growth of pigs was not affected by feeding GM maize. Alterations in immune responses were detected; however, their biologic relevance is questionable.Maria C WalshStefan G BuzoianuGillian E GardinerMary C ReaEva GelencsérAnna JánosiMichelle M EpsteinR Paul RossPeadar G LawlorPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 11, p e27177 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Maria C Walsh Stefan G Buzoianu Gillian E Gardiner Mary C Rea Eva Gelencsér Anna Jánosi Michelle M Epstein R Paul Ross Peadar G Lawlor Fate of transgenic DNA from orally administered Bt MON810 maize and effects on immune response and growth in pigs.
description	We assessed the effect of short-term feeding of genetically modified (GM: Bt MON810) maize on immune responses and growth in weanling pigs and determined the fate of the transgenic DNA and protein in-vivo. Pigs were fed a diet containing 38.9% GM or non-GM isogenic parent line maize for 31 days. We observed that IL-12 and IFNγ production from mitogenic stimulated peripheral blood mononuclear cells decreased (P<0.10) following 31 days of GM maize exposure. While Cry1Ab-specific IgG and IgA were not detected in the plasma of GM maize-fed pigs, the detection of the cry1Ab gene and protein was limited to the gastrointestinal digesta and was not found in the kidneys, liver, spleen, muscle, heart or blood. Feeding GM maize to weanling pigs had no effect on growth performance or body weight. IL-6 and IL-4 production from isolated splenocytes were increased (P<0.05) in response to feeding GM maize while the proportion of CD4(+) T cells in the spleen decreased. In the ileum, the proportion of B cells and macrophages decreased while the proportion of CD4(+) T cells increased in GM maize-fed pigs. IL-8 and IL-4 production from isolated intraepithelial and lamina propria lymphocytes were also increased (P<0.05) in response to feeding GM maize. In conclusion, there was no evidence of cry1Ab gene or protein translocation to the organs and blood of weaning pigs. The growth of pigs was not affected by feeding GM maize. Alterations in immune responses were detected; however, their biologic relevance is questionable.
format	article
author	Maria C Walsh Stefan G Buzoianu Gillian E Gardiner Mary C Rea Eva Gelencsér Anna Jánosi Michelle M Epstein R Paul Ross Peadar G Lawlor
author_facet	Maria C Walsh Stefan G Buzoianu Gillian E Gardiner Mary C Rea Eva Gelencsér Anna Jánosi Michelle M Epstein R Paul Ross Peadar G Lawlor
author_sort	Maria C Walsh
title	Fate of transgenic DNA from orally administered Bt MON810 maize and effects on immune response and growth in pigs.
title_short	Fate of transgenic DNA from orally administered Bt MON810 maize and effects on immune response and growth in pigs.
title_full	Fate of transgenic DNA from orally administered Bt MON810 maize and effects on immune response and growth in pigs.
title_fullStr	Fate of transgenic DNA from orally administered Bt MON810 maize and effects on immune response and growth in pigs.
title_full_unstemmed	Fate of transgenic DNA from orally administered Bt MON810 maize and effects on immune response and growth in pigs.
title_sort	fate of transgenic dna from orally administered bt mon810 maize and effects on immune response and growth in pigs.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/a75b611a11ac46efb2c41b30f9f16a80
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Fate of transgenic DNA from orally administered Bt MON810 maize and effects on immune response and growth in pigs.

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