Label-free stimulated Raman scattering microscopy visualizes changes in intracellular morphology during human epidermal keratinocyte differentiation

Abstract Epidermal keratinocyte (KC) differentiation, which involves the process from proliferation to cell death for shedding the outermost layer of skin, is crucial for the barrier function of skin. Therefore, in dermatology, it is important to elucidate the epidermal KC differentiation process to...

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Autores principales:	Mariko Egawa, Shinya Iwanaga, Junichi Hosoi, Makiko Goto, Haruyo Yamanishi, Masashi Miyai, Chika Katagiri, Kyoya Tokunaga, Takuya Asai, Yasuyuki Ozeki
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a75f3af27dd6420c8e3ccc42af62acce
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spelling	oai:doaj.org-article:a75f3af27dd6420c8e3ccc42af62acce2021-12-02T15:08:47ZLabel-free stimulated Raman scattering microscopy visualizes changes in intracellular morphology during human epidermal keratinocyte differentiation10.1038/s41598-019-49035-x2045-2322https://doaj.org/article/a75f3af27dd6420c8e3ccc42af62acce2019-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-49035-xhttps://doaj.org/toc/2045-2322Abstract Epidermal keratinocyte (KC) differentiation, which involves the process from proliferation to cell death for shedding the outermost layer of skin, is crucial for the barrier function of skin. Therefore, in dermatology, it is important to elucidate the epidermal KC differentiation process to evaluate the symptom level of diseases and skin conditions. Previous dermatological studies used staining or labelling techniques for this purpose, but they have technological limitations for revealing the entire process of epidermal KC differentiation, especially when applied to humans. Here, we demonstrate label-free visualization of three-dimensional (3D) intracellular morphological changes of ex vivo human epidermis during epidermal KC differentiation using stimulated Raman scattering (SRS) microscopy. Specifically, we observed changes in nuclei during the initial enucleation process in which the nucleus is digested prior to flattening. Furthermore, we found holes left behind by improperly digested nuclei in the stratum corneum, suggesting abnormal differentiation. Our findings indicate the great potential of SRS microscopy for discrimination of the degree of epidermal KC differentiation.Mariko EgawaShinya IwanagaJunichi HosoiMakiko GotoHaruyo YamanishiMasashi MiyaiChika KatagiriKyoya TokunagaTakuya AsaiYasuyuki OzekiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-10 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mariko Egawa Shinya Iwanaga Junichi Hosoi Makiko Goto Haruyo Yamanishi Masashi Miyai Chika Katagiri Kyoya Tokunaga Takuya Asai Yasuyuki Ozeki Label-free stimulated Raman scattering microscopy visualizes changes in intracellular morphology during human epidermal keratinocyte differentiation
description	Abstract Epidermal keratinocyte (KC) differentiation, which involves the process from proliferation to cell death for shedding the outermost layer of skin, is crucial for the barrier function of skin. Therefore, in dermatology, it is important to elucidate the epidermal KC differentiation process to evaluate the symptom level of diseases and skin conditions. Previous dermatological studies used staining or labelling techniques for this purpose, but they have technological limitations for revealing the entire process of epidermal KC differentiation, especially when applied to humans. Here, we demonstrate label-free visualization of three-dimensional (3D) intracellular morphological changes of ex vivo human epidermis during epidermal KC differentiation using stimulated Raman scattering (SRS) microscopy. Specifically, we observed changes in nuclei during the initial enucleation process in which the nucleus is digested prior to flattening. Furthermore, we found holes left behind by improperly digested nuclei in the stratum corneum, suggesting abnormal differentiation. Our findings indicate the great potential of SRS microscopy for discrimination of the degree of epidermal KC differentiation.
format	article
author	Mariko Egawa Shinya Iwanaga Junichi Hosoi Makiko Goto Haruyo Yamanishi Masashi Miyai Chika Katagiri Kyoya Tokunaga Takuya Asai Yasuyuki Ozeki
author_facet	Mariko Egawa Shinya Iwanaga Junichi Hosoi Makiko Goto Haruyo Yamanishi Masashi Miyai Chika Katagiri Kyoya Tokunaga Takuya Asai Yasuyuki Ozeki
author_sort	Mariko Egawa
title	Label-free stimulated Raman scattering microscopy visualizes changes in intracellular morphology during human epidermal keratinocyte differentiation
title_short	Label-free stimulated Raman scattering microscopy visualizes changes in intracellular morphology during human epidermal keratinocyte differentiation
title_full	Label-free stimulated Raman scattering microscopy visualizes changes in intracellular morphology during human epidermal keratinocyte differentiation
title_fullStr	Label-free stimulated Raman scattering microscopy visualizes changes in intracellular morphology during human epidermal keratinocyte differentiation
title_full_unstemmed	Label-free stimulated Raman scattering microscopy visualizes changes in intracellular morphology during human epidermal keratinocyte differentiation
title_sort	label-free stimulated raman scattering microscopy visualizes changes in intracellular morphology during human epidermal keratinocyte differentiation
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/a75f3af27dd6420c8e3ccc42af62acce
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Label-free stimulated Raman scattering microscopy visualizes changes in intracellular morphology during human epidermal keratinocyte differentiation

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