Terminal platelet production is regulated by von Willebrand factor.

It is established that proplatelets are formed from mature megakaryocytes (MK) as intermediates before platelet production. Recently, the presence of proplatelets was described in blood incubated in static conditions. We have previously demonstrated that platelet and proplatelet formation is upregul...

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Autores principales:	Sonia Poirault-Chassac, Kim Anh Nguyen, Audrey Pietrzyk, Caterina Casari, Agnes Veyradier, Cecile V Denis, Dominique Baruch
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a773e7d01b874c5999caaac0e564a4e5
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spelling	oai:doaj.org-article:a773e7d01b874c5999caaac0e564a4e52021-11-18T07:43:49ZTerminal platelet production is regulated by von Willebrand factor.1932-620310.1371/journal.pone.0063810https://doaj.org/article/a773e7d01b874c5999caaac0e564a4e52013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23737952/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203It is established that proplatelets are formed from mature megakaryocytes (MK) as intermediates before platelet production. Recently, the presence of proplatelets was described in blood incubated in static conditions. We have previously demonstrated that platelet and proplatelet formation is upregulated by MK exposure to high shear rates (1800 s(-1)) on immobilized von Willebrand factor (VWF). The purpose of the present study was to investigate whether VWF is involved in the regulation of terminal platelet production in blood. To this end, Vwf (-/-) mice, a model of severe von Willebrand disease, were used to create a situation in which blood cells circulate in a vascular tree that is completely devoid of VWF. Murine platelets were isolated from Vwf (-/-) and Vwf (+/+) blood, exposed to VWF at 1800 s(-1) in a microfluidic platform, and examined by means of videomicroscopy, as well as fluorescence and activation studies. Proplatelets became visible within 5 minutes, representing 38% of all platelets after 12 minutes and 46% after 28 min. The proportion of proplatelets was 1.8-fold higher in blood from Vwf(-/-) mice than from Vwf(+/+) mice, suggesting a role of VWF in vivo. Fragmentation of these proplatelets into smaller discoid platelets was also observed in real-time. Platelets remained fully activatable by thrombin. Compensation of plasmatic VWF following hydrodynamic gene transfer in Vwf(-/-) mice reduced the percentage of proplatelets to wild-type levels. A thrombocytopenic mouse model was studied in the flow system, 7 days after a single 5-FU injection. Compared to untreated mouse blood, a 2-fold increase in the percentage of proplatelets was detected following exposure to 1800 s(-1) on VWF of samples from mice treated with 5-FU. In conclusion, VWF and shear stress together appear to upregulate proplatelet reorganization and platelet formation. This suggests a new function for VWF in vivo as regulator of bloodstream thrombopoiesis.Sonia Poirault-ChassacKim Anh NguyenAudrey PietrzykCaterina CasariAgnes VeyradierCecile V DenisDominique BaruchPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 5, p e63810 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sonia Poirault-Chassac Kim Anh Nguyen Audrey Pietrzyk Caterina Casari Agnes Veyradier Cecile V Denis Dominique Baruch Terminal platelet production is regulated by von Willebrand factor.
description	It is established that proplatelets are formed from mature megakaryocytes (MK) as intermediates before platelet production. Recently, the presence of proplatelets was described in blood incubated in static conditions. We have previously demonstrated that platelet and proplatelet formation is upregulated by MK exposure to high shear rates (1800 s(-1)) on immobilized von Willebrand factor (VWF). The purpose of the present study was to investigate whether VWF is involved in the regulation of terminal platelet production in blood. To this end, Vwf (-/-) mice, a model of severe von Willebrand disease, were used to create a situation in which blood cells circulate in a vascular tree that is completely devoid of VWF. Murine platelets were isolated from Vwf (-/-) and Vwf (+/+) blood, exposed to VWF at 1800 s(-1) in a microfluidic platform, and examined by means of videomicroscopy, as well as fluorescence and activation studies. Proplatelets became visible within 5 minutes, representing 38% of all platelets after 12 minutes and 46% after 28 min. The proportion of proplatelets was 1.8-fold higher in blood from Vwf(-/-) mice than from Vwf(+/+) mice, suggesting a role of VWF in vivo. Fragmentation of these proplatelets into smaller discoid platelets was also observed in real-time. Platelets remained fully activatable by thrombin. Compensation of plasmatic VWF following hydrodynamic gene transfer in Vwf(-/-) mice reduced the percentage of proplatelets to wild-type levels. A thrombocytopenic mouse model was studied in the flow system, 7 days after a single 5-FU injection. Compared to untreated mouse blood, a 2-fold increase in the percentage of proplatelets was detected following exposure to 1800 s(-1) on VWF of samples from mice treated with 5-FU. In conclusion, VWF and shear stress together appear to upregulate proplatelet reorganization and platelet formation. This suggests a new function for VWF in vivo as regulator of bloodstream thrombopoiesis.
format	article
author	Sonia Poirault-Chassac Kim Anh Nguyen Audrey Pietrzyk Caterina Casari Agnes Veyradier Cecile V Denis Dominique Baruch
author_facet	Sonia Poirault-Chassac Kim Anh Nguyen Audrey Pietrzyk Caterina Casari Agnes Veyradier Cecile V Denis Dominique Baruch
author_sort	Sonia Poirault-Chassac
title	Terminal platelet production is regulated by von Willebrand factor.
title_short	Terminal platelet production is regulated by von Willebrand factor.
title_full	Terminal platelet production is regulated by von Willebrand factor.
title_fullStr	Terminal platelet production is regulated by von Willebrand factor.
title_full_unstemmed	Terminal platelet production is regulated by von Willebrand factor.
title_sort	terminal platelet production is regulated by von willebrand factor.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/a773e7d01b874c5999caaac0e564a4e5
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Terminal platelet production is regulated by von Willebrand factor.

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