Mapping distribution of cysts of recent dinoflagellate and Cochlodinium polykrikoides using next-generation sequencing and morphological approaches in South Sea, Korea

Abstract The total dinoflagellate cyst community and the cysts of Cochlodinium polykrikoides in the surface sediments of South Sea (Tongyeong coast), South Korea, were analysed using next-generation sequencing (NGS) and morphological approaches. Dinoflagellate cysts can be highly abundant (111–4,087...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Seung Won Jung, Donhyug Kang, Hyun-Jung Kim, Hyeon Ho Shin, Joon Sang Park, So Yun Park, Taek-Kyun Lee
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a7769f4f78f7484e9cc6564519b6038f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a7769f4f78f7484e9cc6564519b6038f
record_format	dspace
spelling	oai:doaj.org-article:a7769f4f78f7484e9cc6564519b6038f2021-12-02T15:08:50ZMapping distribution of cysts of recent dinoflagellate and Cochlodinium polykrikoides using next-generation sequencing and morphological approaches in South Sea, Korea10.1038/s41598-018-25345-42045-2322https://doaj.org/article/a7769f4f78f7484e9cc6564519b6038f2018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25345-4https://doaj.org/toc/2045-2322Abstract The total dinoflagellate cyst community and the cysts of Cochlodinium polykrikoides in the surface sediments of South Sea (Tongyeong coast), South Korea, were analysed using next-generation sequencing (NGS) and morphological approaches. Dinoflagellate cysts can be highly abundant (111–4,087 cysts g−1 dry weight) and have diverse species composition. A total of 35 taxa of dinoflagellate cysts representing 16 genera, 21 species (including four unconfirmed species), and 14 complex species were identified by NGS analysis. Cysts of Scrippsiella spp (mostly Scrippsiella trochoidea) were the most dominant and Polykrikos schwartzii, Pentapharsodinium dalei, Ensiculifera carinata, and Alexandrium catenella/tamarense were common. Thus, a combination of NGS and morphological analysis is effective for studying the cyst communities present in a given environment. Although C. polykrikoides developed massive blooms during 2013–2014, microscopy revealed low density of their cysts, whereas no cysts were detected by NGS. However, the vegetative C. polykrikoides not appeared during 2015–2017 in spite of the observation of C. polykrikoides cysts. This suggests that the C. polykrikoides blooms were not due to development of their cysts but to other factors such as currents transporting them to a marine environment suitable for their growth.Seung Won JungDonhyug KangHyun-Jung KimHyeon Ho ShinJoon Sang ParkSo Yun ParkTaek-Kyun LeeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Seung Won Jung Donhyug Kang Hyun-Jung Kim Hyeon Ho Shin Joon Sang Park So Yun Park Taek-Kyun Lee Mapping distribution of cysts of recent dinoflagellate and Cochlodinium polykrikoides using next-generation sequencing and morphological approaches in South Sea, Korea
description	Abstract The total dinoflagellate cyst community and the cysts of Cochlodinium polykrikoides in the surface sediments of South Sea (Tongyeong coast), South Korea, were analysed using next-generation sequencing (NGS) and morphological approaches. Dinoflagellate cysts can be highly abundant (111–4,087 cysts g−1 dry weight) and have diverse species composition. A total of 35 taxa of dinoflagellate cysts representing 16 genera, 21 species (including four unconfirmed species), and 14 complex species were identified by NGS analysis. Cysts of Scrippsiella spp (mostly Scrippsiella trochoidea) were the most dominant and Polykrikos schwartzii, Pentapharsodinium dalei, Ensiculifera carinata, and Alexandrium catenella/tamarense were common. Thus, a combination of NGS and morphological analysis is effective for studying the cyst communities present in a given environment. Although C. polykrikoides developed massive blooms during 2013–2014, microscopy revealed low density of their cysts, whereas no cysts were detected by NGS. However, the vegetative C. polykrikoides not appeared during 2015–2017 in spite of the observation of C. polykrikoides cysts. This suggests that the C. polykrikoides blooms were not due to development of their cysts but to other factors such as currents transporting them to a marine environment suitable for their growth.
format	article
author	Seung Won Jung Donhyug Kang Hyun-Jung Kim Hyeon Ho Shin Joon Sang Park So Yun Park Taek-Kyun Lee
author_facet	Seung Won Jung Donhyug Kang Hyun-Jung Kim Hyeon Ho Shin Joon Sang Park So Yun Park Taek-Kyun Lee
author_sort	Seung Won Jung
title	Mapping distribution of cysts of recent dinoflagellate and Cochlodinium polykrikoides using next-generation sequencing and morphological approaches in South Sea, Korea
title_short	Mapping distribution of cysts of recent dinoflagellate and Cochlodinium polykrikoides using next-generation sequencing and morphological approaches in South Sea, Korea
title_full	Mapping distribution of cysts of recent dinoflagellate and Cochlodinium polykrikoides using next-generation sequencing and morphological approaches in South Sea, Korea
title_fullStr	Mapping distribution of cysts of recent dinoflagellate and Cochlodinium polykrikoides using next-generation sequencing and morphological approaches in South Sea, Korea
title_full_unstemmed	Mapping distribution of cysts of recent dinoflagellate and Cochlodinium polykrikoides using next-generation sequencing and morphological approaches in South Sea, Korea
title_sort	mapping distribution of cysts of recent dinoflagellate and cochlodinium polykrikoides using next-generation sequencing and morphological approaches in south sea, korea
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/a7769f4f78f7484e9cc6564519b6038f
work_keys_str_mv	AT seungwonjung mappingdistributionofcystsofrecentdinoflagellateandcochlodiniumpolykrikoidesusingnextgenerationsequencingandmorphologicalapproachesinsouthseakorea AT donhyugkang mappingdistributionofcystsofrecentdinoflagellateandcochlodiniumpolykrikoidesusingnextgenerationsequencingandmorphologicalapproachesinsouthseakorea AT hyunjungkim mappingdistributionofcystsofrecentdinoflagellateandcochlodiniumpolykrikoidesusingnextgenerationsequencingandmorphologicalapproachesinsouthseakorea AT hyeonhoshin mappingdistributionofcystsofrecentdinoflagellateandcochlodiniumpolykrikoidesusingnextgenerationsequencingandmorphologicalapproachesinsouthseakorea AT joonsangpark mappingdistributionofcystsofrecentdinoflagellateandcochlodiniumpolykrikoidesusingnextgenerationsequencingandmorphologicalapproachesinsouthseakorea AT soyunpark mappingdistributionofcystsofrecentdinoflagellateandcochlodiniumpolykrikoidesusingnextgenerationsequencingandmorphologicalapproachesinsouthseakorea AT taekkyunlee mappingdistributionofcystsofrecentdinoflagellateandcochlodiniumpolykrikoidesusingnextgenerationsequencingandmorphologicalapproachesinsouthseakorea
_version_	1718388019698860032

Mapping distribution of cysts of recent dinoflagellate and Cochlodinium polykrikoides using next-generation sequencing and morphological approaches in South Sea, Korea

Ejemplares similares