Acute ocular hypertension disrupts barrier integrity and pump function in rat corneal endothelial cells

Abstract Acute ocular hypertension (AOH) frequently compromises corneal endothelial cell (CEC) function in clinical practice. This type of stress induces corneal oedema and a decrease in the corneal endothelial cell density (ECD). The anterior chamber of the right eye of Sprague-Dawley rats was irri...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Xian Li, Zhenhao Zhang, Lijun Ye, Jufeng Meng, Zhongyang Zhao, Zuguo Liu, Jiaoyue Hu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a781dafdcb064c96b8c331d4650b01a2
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a781dafdcb064c96b8c331d4650b01a2
record_format	dspace
spelling	oai:doaj.org-article:a781dafdcb064c96b8c331d4650b01a22021-12-02T16:06:24ZAcute ocular hypertension disrupts barrier integrity and pump function in rat corneal endothelial cells10.1038/s41598-017-07534-92045-2322https://doaj.org/article/a781dafdcb064c96b8c331d4650b01a22017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07534-9https://doaj.org/toc/2045-2322Abstract Acute ocular hypertension (AOH) frequently compromises corneal endothelial cell (CEC) function in clinical practice. This type of stress induces corneal oedema and a decrease in the corneal endothelial cell density (ECD). The anterior chamber of the right eye of Sprague-Dawley rats was irrigated with Balanced Salt Solution (BSS) for two hours, and the left eye served as a control to determine the time-dependent effects of AOH on endothelial cell morphology and function. The average intraocular pressure (IOP) increased to 82.6 ± 2.3 mmHg (normal range: 10.2 ± 0.4 mmHg) during anterior irrigation. Very soon after initiating irrigation, corneal oedema became evident and the cornea exhibited a significant increase in permeability to FITC-dextran. The peripheral ECD was significantly reduced, and the morphology of CECs became irregular and multiform. The structures of the zonula occludens-1 (ZO-1) and F-actin were severely disrupted. In addtion, Na,K-ATPase exhibited a dispersed expression pattern. Two days after irrigation, obvious CEC proliferation was observed, the ECD recovered to a normal level, and F-actin was dispersed throughout the cytoplasm. Seven days later, the CEC structure and function were nearly normalized. Based on the results obtained using this model, an acute IOP crisis exerts transient deleterious effects on CEC structure and function in rats.Xian LiZhenhao ZhangLijun YeJufeng MengZhongyang ZhaoZuguo LiuJiaoyue HuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xian Li Zhenhao Zhang Lijun Ye Jufeng Meng Zhongyang Zhao Zuguo Liu Jiaoyue Hu Acute ocular hypertension disrupts barrier integrity and pump function in rat corneal endothelial cells
description	Abstract Acute ocular hypertension (AOH) frequently compromises corneal endothelial cell (CEC) function in clinical practice. This type of stress induces corneal oedema and a decrease in the corneal endothelial cell density (ECD). The anterior chamber of the right eye of Sprague-Dawley rats was irrigated with Balanced Salt Solution (BSS) for two hours, and the left eye served as a control to determine the time-dependent effects of AOH on endothelial cell morphology and function. The average intraocular pressure (IOP) increased to 82.6 ± 2.3 mmHg (normal range: 10.2 ± 0.4 mmHg) during anterior irrigation. Very soon after initiating irrigation, corneal oedema became evident and the cornea exhibited a significant increase in permeability to FITC-dextran. The peripheral ECD was significantly reduced, and the morphology of CECs became irregular and multiform. The structures of the zonula occludens-1 (ZO-1) and F-actin were severely disrupted. In addtion, Na,K-ATPase exhibited a dispersed expression pattern. Two days after irrigation, obvious CEC proliferation was observed, the ECD recovered to a normal level, and F-actin was dispersed throughout the cytoplasm. Seven days later, the CEC structure and function were nearly normalized. Based on the results obtained using this model, an acute IOP crisis exerts transient deleterious effects on CEC structure and function in rats.
format	article
author	Xian Li Zhenhao Zhang Lijun Ye Jufeng Meng Zhongyang Zhao Zuguo Liu Jiaoyue Hu
author_facet	Xian Li Zhenhao Zhang Lijun Ye Jufeng Meng Zhongyang Zhao Zuguo Liu Jiaoyue Hu
author_sort	Xian Li
title	Acute ocular hypertension disrupts barrier integrity and pump function in rat corneal endothelial cells
title_short	Acute ocular hypertension disrupts barrier integrity and pump function in rat corneal endothelial cells
title_full	Acute ocular hypertension disrupts barrier integrity and pump function in rat corneal endothelial cells
title_fullStr	Acute ocular hypertension disrupts barrier integrity and pump function in rat corneal endothelial cells
title_full_unstemmed	Acute ocular hypertension disrupts barrier integrity and pump function in rat corneal endothelial cells
title_sort	acute ocular hypertension disrupts barrier integrity and pump function in rat corneal endothelial cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/a781dafdcb064c96b8c331d4650b01a2
work_keys_str_mv	AT xianli acuteocularhypertensiondisruptsbarrierintegrityandpumpfunctioninratcornealendothelialcells AT zhenhaozhang acuteocularhypertensiondisruptsbarrierintegrityandpumpfunctioninratcornealendothelialcells AT lijunye acuteocularhypertensiondisruptsbarrierintegrityandpumpfunctioninratcornealendothelialcells AT jufengmeng acuteocularhypertensiondisruptsbarrierintegrityandpumpfunctioninratcornealendothelialcells AT zhongyangzhao acuteocularhypertensiondisruptsbarrierintegrityandpumpfunctioninratcornealendothelialcells AT zuguoliu acuteocularhypertensiondisruptsbarrierintegrityandpumpfunctioninratcornealendothelialcells AT jiaoyuehu acuteocularhypertensiondisruptsbarrierintegrityandpumpfunctioninratcornealendothelialcells
_version_	1718385044794376192

Acute ocular hypertension disrupts barrier integrity and pump function in rat corneal endothelial cells

Ejemplares similares