Improvement in bladder dysfunction after bladder transplantation of amniotic fluid stem cells in diabetic rats

Abstract To examine the effects of human amniotic fluid stem cells (hAFSCs) transplantation on bladder function and molecular changes in diabetic rats, 60 female Sprague-Dawley rats were used for study. Three groups were assigned including sham control rats, streptozotocin (STZ, 60 mg/kg)-induced di...

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Autores principales:	Ching-Chung Liang, Sheng-Wen Steven Shaw, Yung-Hsin Huang, Yi-Hao Lin, Tsong-Hai Lee
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a7831d9e99464055b4a5ff482c1e976f
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spelling	oai:doaj.org-article:a7831d9e99464055b4a5ff482c1e976f2021-12-02T15:08:35ZImprovement in bladder dysfunction after bladder transplantation of amniotic fluid stem cells in diabetic rats10.1038/s41598-018-20512-z2045-2322https://doaj.org/article/a7831d9e99464055b4a5ff482c1e976f2018-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-20512-zhttps://doaj.org/toc/2045-2322Abstract To examine the effects of human amniotic fluid stem cells (hAFSCs) transplantation on bladder function and molecular changes in diabetic rats, 60 female Sprague-Dawley rats were used for study. Three groups were assigned including sham control rats, streptozotocin (STZ, 60 mg/kg)-induced diabetic rats and STZ-induced diabetic rats plus bladder hAFSCs transplantation. Compared to controls, diabetic rats had decreased body weight but increased bladder weight. Cystometries showed non-voiding contraction, residual volume, voided volume and intercontraction interval increased significantly in diabetic rats at week 4 and 12 after DM induction, but improved after hAFSCs transplantation. The immunoreactivities and mRNAs of nerve growth factor (NGF) decreased significantly in diabetic bladder at week 4 and 12 after DM induction, but recovered after hAFSCs transplantation. The immunoreactivities and mRNAs of M2 and M3 muscarinic receptor increased significantly in diabetic bladder at week 4 after DM induction but recovered after hAFSCs transplantation. The immunoreactivity of 8-hydroxy-20-deoxyguanosine increased significantly in diabetic bladder at week 4 and 12 after DM induction but reduced after hAFSCs transplantation. The present study showed bladder dysfunction in STZ-induced diabetic rats could be improved by hAFSCs transplantation into bladder, which may be related to the recovery of bladder NGF and muscarinic receptors.Ching-Chung LiangSheng-Wen Steven ShawYung-Hsin HuangYi-Hao LinTsong-Hai LeeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-11 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ching-Chung Liang Sheng-Wen Steven Shaw Yung-Hsin Huang Yi-Hao Lin Tsong-Hai Lee Improvement in bladder dysfunction after bladder transplantation of amniotic fluid stem cells in diabetic rats
description	Abstract To examine the effects of human amniotic fluid stem cells (hAFSCs) transplantation on bladder function and molecular changes in diabetic rats, 60 female Sprague-Dawley rats were used for study. Three groups were assigned including sham control rats, streptozotocin (STZ, 60 mg/kg)-induced diabetic rats and STZ-induced diabetic rats plus bladder hAFSCs transplantation. Compared to controls, diabetic rats had decreased body weight but increased bladder weight. Cystometries showed non-voiding contraction, residual volume, voided volume and intercontraction interval increased significantly in diabetic rats at week 4 and 12 after DM induction, but improved after hAFSCs transplantation. The immunoreactivities and mRNAs of nerve growth factor (NGF) decreased significantly in diabetic bladder at week 4 and 12 after DM induction, but recovered after hAFSCs transplantation. The immunoreactivities and mRNAs of M2 and M3 muscarinic receptor increased significantly in diabetic bladder at week 4 after DM induction but recovered after hAFSCs transplantation. The immunoreactivity of 8-hydroxy-20-deoxyguanosine increased significantly in diabetic bladder at week 4 and 12 after DM induction but reduced after hAFSCs transplantation. The present study showed bladder dysfunction in STZ-induced diabetic rats could be improved by hAFSCs transplantation into bladder, which may be related to the recovery of bladder NGF and muscarinic receptors.
format	article
author	Ching-Chung Liang Sheng-Wen Steven Shaw Yung-Hsin Huang Yi-Hao Lin Tsong-Hai Lee
author_facet	Ching-Chung Liang Sheng-Wen Steven Shaw Yung-Hsin Huang Yi-Hao Lin Tsong-Hai Lee
author_sort	Ching-Chung Liang
title	Improvement in bladder dysfunction after bladder transplantation of amniotic fluid stem cells in diabetic rats
title_short	Improvement in bladder dysfunction after bladder transplantation of amniotic fluid stem cells in diabetic rats
title_full	Improvement in bladder dysfunction after bladder transplantation of amniotic fluid stem cells in diabetic rats
title_fullStr	Improvement in bladder dysfunction after bladder transplantation of amniotic fluid stem cells in diabetic rats
title_full_unstemmed	Improvement in bladder dysfunction after bladder transplantation of amniotic fluid stem cells in diabetic rats
title_sort	improvement in bladder dysfunction after bladder transplantation of amniotic fluid stem cells in diabetic rats
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/a7831d9e99464055b4a5ff482c1e976f
work_keys_str_mv	AT chingchungliang improvementinbladderdysfunctionafterbladdertransplantationofamnioticfluidstemcellsindiabeticrats AT shengwenstevenshaw improvementinbladderdysfunctionafterbladdertransplantationofamnioticfluidstemcellsindiabeticrats AT yunghsinhuang improvementinbladderdysfunctionafterbladdertransplantationofamnioticfluidstemcellsindiabeticrats AT yihaolin improvementinbladderdysfunctionafterbladdertransplantationofamnioticfluidstemcellsindiabeticrats AT tsonghailee improvementinbladderdysfunctionafterbladdertransplantationofamnioticfluidstemcellsindiabeticrats
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Improvement in bladder dysfunction after bladder transplantation of amniotic fluid stem cells in diabetic rats

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