Phage-Resistant Bacteria Reveal a Role for Potassium in Root Colonization

ABSTRACT Bacteriophage predation is an important factor in bacterial community dynamics and evolution. Phage-bacterium interaction has mainly been studied in lab cultures, while dynamics in natural habitats, and especially in the plant root niche, are underexplored. To better understand this process...

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Autores principales:	Elhanan Tzipilevich, Philip N. Benfey
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2021
Materias:	Bacillus subtilis bacteriophage, evolution biofilms plant-microbe interactions Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/a78d5eb2dd694dfc870a291354ab3227
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spelling	oai:doaj.org-article:a78d5eb2dd694dfc870a291354ab32272021-11-10T18:37:51ZPhage-Resistant Bacteria Reveal a Role for Potassium in Root Colonization10.1128/mBio.01403-212150-7511https://doaj.org/article/a78d5eb2dd694dfc870a291354ab32272021-08-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.01403-21https://doaj.org/toc/2150-7511ABSTRACT Bacteriophage predation is an important factor in bacterial community dynamics and evolution. Phage-bacterium interaction has mainly been studied in lab cultures, while dynamics in natural habitats, and especially in the plant root niche, are underexplored. To better understand this process, we characterized infection of the soil bacterium Bacillus subtilis NCBI 3610 by the lytic phage SPO1 during growth in LB medium and compared it to root colonization. Resistance in vitro was primarily through modification of the phage receptor. However, this type of resistance reduced the ability to colonize the root. From a line that survived phage infection while retaining the ability to colonize the root, we identified a new phage resistance mechanism involving potassium (K+) ion influx modulation and enhanced biofilm formation. Furthermore, we show that potassium serves as a stimulator of root colonization among diverse growth-promoting bacilli species, with implications for plant health. IMPORTANCE Bacteriophage predation is an important factor in bacterial community dynamics and evolution. Phage-bacterium interaction has mainly been studied in lab cultures, while dynamics in natural habitats, and especially in the plant root niche, are underexplored. To better understand this process, we characterized infection of the soil bacterium Bacillus subtilis NCBI 3610 by the lytic phage SPO1 during growth in LB medium and compared it to root colonization. Resistance in vitro was primarily through modification of the phage receptor. However, this type of resistance reduced the ability to colonize the root. From a line that survived phage infection while retaining the ability to colonize the root, we identified a new phage resistance mechanism involving potassium (K+) ion influx modulation and enhanced biofilm formation. Furthermore, we show that potassium serves as a stimulator of root colonization among diverse growth-promoting bacilli species, with implications for plant health.Elhanan TzipilevichPhilip N. BenfeyAmerican Society for MicrobiologyarticleBacillus subtilisbacteriophage, evolutionbiofilmsplant-microbe interactionsMicrobiologyQR1-502ENmBio, Vol 12, Iss 4 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Bacillus subtilis bacteriophage, evolution biofilms plant-microbe interactions Microbiology QR1-502
spellingShingle	Bacillus subtilis bacteriophage, evolution biofilms plant-microbe interactions Microbiology QR1-502 Elhanan Tzipilevich Philip N. Benfey Phage-Resistant Bacteria Reveal a Role for Potassium in Root Colonization
description	ABSTRACT Bacteriophage predation is an important factor in bacterial community dynamics and evolution. Phage-bacterium interaction has mainly been studied in lab cultures, while dynamics in natural habitats, and especially in the plant root niche, are underexplored. To better understand this process, we characterized infection of the soil bacterium Bacillus subtilis NCBI 3610 by the lytic phage SPO1 during growth in LB medium and compared it to root colonization. Resistance in vitro was primarily through modification of the phage receptor. However, this type of resistance reduced the ability to colonize the root. From a line that survived phage infection while retaining the ability to colonize the root, we identified a new phage resistance mechanism involving potassium (K+) ion influx modulation and enhanced biofilm formation. Furthermore, we show that potassium serves as a stimulator of root colonization among diverse growth-promoting bacilli species, with implications for plant health. IMPORTANCE Bacteriophage predation is an important factor in bacterial community dynamics and evolution. Phage-bacterium interaction has mainly been studied in lab cultures, while dynamics in natural habitats, and especially in the plant root niche, are underexplored. To better understand this process, we characterized infection of the soil bacterium Bacillus subtilis NCBI 3610 by the lytic phage SPO1 during growth in LB medium and compared it to root colonization. Resistance in vitro was primarily through modification of the phage receptor. However, this type of resistance reduced the ability to colonize the root. From a line that survived phage infection while retaining the ability to colonize the root, we identified a new phage resistance mechanism involving potassium (K+) ion influx modulation and enhanced biofilm formation. Furthermore, we show that potassium serves as a stimulator of root colonization among diverse growth-promoting bacilli species, with implications for plant health.
format	article
author	Elhanan Tzipilevich Philip N. Benfey
author_facet	Elhanan Tzipilevich Philip N. Benfey
author_sort	Elhanan Tzipilevich
title	Phage-Resistant Bacteria Reveal a Role for Potassium in Root Colonization
title_short	Phage-Resistant Bacteria Reveal a Role for Potassium in Root Colonization
title_full	Phage-Resistant Bacteria Reveal a Role for Potassium in Root Colonization
title_fullStr	Phage-Resistant Bacteria Reveal a Role for Potassium in Root Colonization
title_full_unstemmed	Phage-Resistant Bacteria Reveal a Role for Potassium in Root Colonization
title_sort	phage-resistant bacteria reveal a role for potassium in root colonization
publisher	American Society for Microbiology
publishDate	2021
url	https://doaj.org/article/a78d5eb2dd694dfc870a291354ab3227
work_keys_str_mv	AT elhanantzipilevich phageresistantbacteriarevealaroleforpotassiuminrootcolonization AT philipnbenfey phageresistantbacteriarevealaroleforpotassiuminrootcolonization
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Phage-Resistant Bacteria Reveal a Role for Potassium in Root Colonization

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