Development of rapid and cost-effective top-loading device for the detection of anti-SARS-CoV-2 IgG/IgM antibodies

Abstract Infection with SARS-CoV-2, the Betacoronavirus, caused a pandemic that affected the globe negatively. The gold method, RT-PCR, can detect SARS-CoV-2 but it is time-consuming and needs sophisticated equipment and professional personnel. On the other hand, rapid tests offer fast results and c...

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Autores principales:	Essam H. Ibrahim, Hamed A. Ghramh, Mona Kilany
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a790bf70a0a0417f9d4ceb195360cc95
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spelling	oai:doaj.org-article:a790bf70a0a0417f9d4ceb195360cc952021-12-02T16:26:29ZDevelopment of rapid and cost-effective top-loading device for the detection of anti-SARS-CoV-2 IgG/IgM antibodies10.1038/s41598-021-94444-62045-2322https://doaj.org/article/a790bf70a0a0417f9d4ceb195360cc952021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94444-6https://doaj.org/toc/2045-2322Abstract Infection with SARS-CoV-2, the Betacoronavirus, caused a pandemic that affected the globe negatively. The gold method, RT-PCR, can detect SARS-CoV-2 but it is time-consuming and needs sophisticated equipment and professional personnel. On the other hand, rapid tests offer fast results and can detect anti-SARS-CoV-2 antibodies (Abs). The aim of this study is to develop a new rapid and cost-effective method for the detection of anti-SARS-CoV-2 IgG/IgM Abs. A new top-loading detection device was developed and composed of a small piece of plastic (25 × 25 × 0.5 mm) with an opening in the center, a piece of nitrocellulose (NC) membrane enough to block the opening from one side and adhesive tape to affix the NC to the plastic piece. The NC is blotted with anti-human IgG/IgM and rabbit serum. The device was evaluated against a commercially available IgG/IgM ELISA detection kit using normal, Covid-19-positive, HCV, HBV, and Cytomegalovirus-positive sera. Outcomes demonstrated simplicity, reproducibility, and accuracy of the new device and results can be obtained in less than 5 min. We anticipate our developed assay method to be used widely in point of care before deciding on the use of expensive nucleic acid assays.Essam H. IbrahimHamed A. GhramhMona KilanyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Essam H. Ibrahim Hamed A. Ghramh Mona Kilany Development of rapid and cost-effective top-loading device for the detection of anti-SARS-CoV-2 IgG/IgM antibodies
description	Abstract Infection with SARS-CoV-2, the Betacoronavirus, caused a pandemic that affected the globe negatively. The gold method, RT-PCR, can detect SARS-CoV-2 but it is time-consuming and needs sophisticated equipment and professional personnel. On the other hand, rapid tests offer fast results and can detect anti-SARS-CoV-2 antibodies (Abs). The aim of this study is to develop a new rapid and cost-effective method for the detection of anti-SARS-CoV-2 IgG/IgM Abs. A new top-loading detection device was developed and composed of a small piece of plastic (25 × 25 × 0.5 mm) with an opening in the center, a piece of nitrocellulose (NC) membrane enough to block the opening from one side and adhesive tape to affix the NC to the plastic piece. The NC is blotted with anti-human IgG/IgM and rabbit serum. The device was evaluated against a commercially available IgG/IgM ELISA detection kit using normal, Covid-19-positive, HCV, HBV, and Cytomegalovirus-positive sera. Outcomes demonstrated simplicity, reproducibility, and accuracy of the new device and results can be obtained in less than 5 min. We anticipate our developed assay method to be used widely in point of care before deciding on the use of expensive nucleic acid assays.
format	article
author	Essam H. Ibrahim Hamed A. Ghramh Mona Kilany
author_facet	Essam H. Ibrahim Hamed A. Ghramh Mona Kilany
author_sort	Essam H. Ibrahim
title	Development of rapid and cost-effective top-loading device for the detection of anti-SARS-CoV-2 IgG/IgM antibodies
title_short	Development of rapid and cost-effective top-loading device for the detection of anti-SARS-CoV-2 IgG/IgM antibodies
title_full	Development of rapid and cost-effective top-loading device for the detection of anti-SARS-CoV-2 IgG/IgM antibodies
title_fullStr	Development of rapid and cost-effective top-loading device for the detection of anti-SARS-CoV-2 IgG/IgM antibodies
title_full_unstemmed	Development of rapid and cost-effective top-loading device for the detection of anti-SARS-CoV-2 IgG/IgM antibodies
title_sort	development of rapid and cost-effective top-loading device for the detection of anti-sars-cov-2 igg/igm antibodies
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/a790bf70a0a0417f9d4ceb195360cc95
work_keys_str_mv	AT essamhibrahim developmentofrapidandcosteffectivetoploadingdeviceforthedetectionofantisarscov2iggigmantibodies AT hamedaghramh developmentofrapidandcosteffectivetoploadingdeviceforthedetectionofantisarscov2iggigmantibodies AT monakilany developmentofrapidandcosteffectivetoploadingdeviceforthedetectionofantisarscov2iggigmantibodies
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Development of rapid and cost-effective top-loading device for the detection of anti-SARS-CoV-2 IgG/IgM antibodies

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