INTERACTION OF TRANSCRIPTION FACTORS PAX-5 AND STAT6 IN PATHOGENESIS OF ALLERGIC BRONCHIAL ASTHMA

The aim of this work was to study an opportunity of coordinated expression of PAX-5 and STAT6 in bronchial asthma (BA) development. 35 healthy controls, 83 patients with allergic bronchial asthma (ABA) and 63 patients with nonallergic bronchial asthma (NABA) o different severity were observed. To ev...

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Autores principales:	V. N. Mineev, L. N. Sorokina, M. A. Nyoma, A. V. Eremeeva
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2014
Materias:	bronchial asthma pax-5 stat6 pstat6 lymphocytes Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/a79224dc065a4648b2f92d191af2273f
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spelling	oai:doaj.org-article:a79224dc065a4648b2f92d191af2273f2021-11-18T08:03:37ZINTERACTION OF TRANSCRIPTION FACTORS PAX-5 AND STAT6 IN PATHOGENESIS OF ALLERGIC BRONCHIAL ASTHMA1563-06252313-741X10.15789/1563-0625-2014-1-35-42https://doaj.org/article/a79224dc065a4648b2f92d191af2273f2014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/20https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XThe aim of this work was to study an opportunity of coordinated expression of PAX-5 and STAT6 in bronchial asthma (BA) development. 35 healthy controls, 83 patients with allergic bronchial asthma (ABA) and 63 patients with nonallergic bronchial asthma (NABA) o different severity were observed. To evaluate PAX- 5 and β-actin mRNA levels, a reverse transcription-PCR with endpoint detection was applied. To determine STAT6 and pSTAT6 protein levels, ECL Western blotting protocols (Amersham) was used. A negative correlation was revealed between РАХ-5 and STAT6 mRNA expression levels in control group and in ABA. In NABA patients, the appropriate correlations were nonsignificant. Expression of РАХ-5 mRNA in ABA with normal IgE levels showed a significant negative correlation with that of STAT6. Meanwhile, expression levels of active pSTAT6 form in patients with high IgE and eosinophilia did negatively correlate with РАХ-5 mRNA expression. In ABA, effects of IL-4 caused a more significant decrease of РАХ-5 mRNA as compared with those in control group. The strongest negative correlation between РАХ-5 mRNA expression and STAT6 levels was revealed in groups with higher levels of mRNA РАХ-5 and lower levels of STAT6. The revealed negative correlations between STAT6 levels and РАХ-5 mRNA expression suggest that STAT6 may inhibit PAX-5 transcription in ABA group, thus, probably, promoting differentiation of B cells into plasma cells.V. N. MineevL. N. SorokinaM. A. NyomaA. V. EremeevaSPb RAACIarticlebronchial asthmapax-5stat6pstat6lymphocytesImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 16, Iss 1, Pp 35-42 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	bronchial asthma pax-5 stat6 pstat6 lymphocytes Immunologic diseases. Allergy RC581-607
spellingShingle	bronchial asthma pax-5 stat6 pstat6 lymphocytes Immunologic diseases. Allergy RC581-607 V. N. Mineev L. N. Sorokina M. A. Nyoma A. V. Eremeeva INTERACTION OF TRANSCRIPTION FACTORS PAX-5 AND STAT6 IN PATHOGENESIS OF ALLERGIC BRONCHIAL ASTHMA
description	The aim of this work was to study an opportunity of coordinated expression of PAX-5 and STAT6 in bronchial asthma (BA) development. 35 healthy controls, 83 patients with allergic bronchial asthma (ABA) and 63 patients with nonallergic bronchial asthma (NABA) o different severity were observed. To evaluate PAX- 5 and β-actin mRNA levels, a reverse transcription-PCR with endpoint detection was applied. To determine STAT6 and pSTAT6 protein levels, ECL Western blotting protocols (Amersham) was used. A negative correlation was revealed between РАХ-5 and STAT6 mRNA expression levels in control group and in ABA. In NABA patients, the appropriate correlations were nonsignificant. Expression of РАХ-5 mRNA in ABA with normal IgE levels showed a significant negative correlation with that of STAT6. Meanwhile, expression levels of active pSTAT6 form in patients with high IgE and eosinophilia did negatively correlate with РАХ-5 mRNA expression. In ABA, effects of IL-4 caused a more significant decrease of РАХ-5 mRNA as compared with those in control group. The strongest negative correlation between РАХ-5 mRNA expression and STAT6 levels was revealed in groups with higher levels of mRNA РАХ-5 and lower levels of STAT6. The revealed negative correlations between STAT6 levels and РАХ-5 mRNA expression suggest that STAT6 may inhibit PAX-5 transcription in ABA group, thus, probably, promoting differentiation of B cells into plasma cells.
format	article
author	V. N. Mineev L. N. Sorokina M. A. Nyoma A. V. Eremeeva
author_facet	V. N. Mineev L. N. Sorokina M. A. Nyoma A. V. Eremeeva
author_sort	V. N. Mineev
title	INTERACTION OF TRANSCRIPTION FACTORS PAX-5 AND STAT6 IN PATHOGENESIS OF ALLERGIC BRONCHIAL ASTHMA
title_short	INTERACTION OF TRANSCRIPTION FACTORS PAX-5 AND STAT6 IN PATHOGENESIS OF ALLERGIC BRONCHIAL ASTHMA
title_full	INTERACTION OF TRANSCRIPTION FACTORS PAX-5 AND STAT6 IN PATHOGENESIS OF ALLERGIC BRONCHIAL ASTHMA
title_fullStr	INTERACTION OF TRANSCRIPTION FACTORS PAX-5 AND STAT6 IN PATHOGENESIS OF ALLERGIC BRONCHIAL ASTHMA
title_full_unstemmed	INTERACTION OF TRANSCRIPTION FACTORS PAX-5 AND STAT6 IN PATHOGENESIS OF ALLERGIC BRONCHIAL ASTHMA
title_sort	interaction of transcription factors pax-5 and stat6 in pathogenesis of allergic bronchial asthma
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/a79224dc065a4648b2f92d191af2273f
work_keys_str_mv	AT vnmineev interactionoftranscriptionfactorspax5andstat6inpathogenesisofallergicbronchialasthma AT lnsorokina interactionoftranscriptionfactorspax5andstat6inpathogenesisofallergicbronchialasthma AT manyoma interactionoftranscriptionfactorspax5andstat6inpathogenesisofallergicbronchialasthma AT averemeeva interactionoftranscriptionfactorspax5andstat6inpathogenesisofallergicbronchialasthma
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INTERACTION OF TRANSCRIPTION FACTORS PAX-5 AND STAT6 IN PATHOGENESIS OF ALLERGIC BRONCHIAL ASTHMA

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