ScreenSeed as a novel high throughput seed germination phenotyping method

Abstract A high throughput phenotyping tool for seed germination, the ScreenSeed technology, was developed with the aim of screening genotype responsiveness and chemical drugs. This technology was presently used with Arabidopsis thaliana seeds to allow characterizing seed samples germination behavio...

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Autores principales:	Nicolas Merieux, Pierre Cordier, Marie-Hélène Wagner, Sylvie Ducournau, Sophie Aligon, Dominique Job, Philippe Grappin, Edwin Grappin
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a7959c52d65746699a5df85f6a0bf621
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spelling	oai:doaj.org-article:a7959c52d65746699a5df85f6a0bf6212021-12-02T14:12:41ZScreenSeed as a novel high throughput seed germination phenotyping method10.1038/s41598-020-79115-22045-2322https://doaj.org/article/a7959c52d65746699a5df85f6a0bf6212021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79115-2https://doaj.org/toc/2045-2322Abstract A high throughput phenotyping tool for seed germination, the ScreenSeed technology, was developed with the aim of screening genotype responsiveness and chemical drugs. This technology was presently used with Arabidopsis thaliana seeds to allow characterizing seed samples germination behavior by incubating seeds in 96-well microplates under defined conditions and detecting radicle protrusion through the seed coat by automated image analysis. This study shows that this technology provides a fast procedure allowing to handle thousands of seeds without compromising repeatability or accuracy of the germination measurements. Potential biases of the experimental protocol were assessed through statistical analyses of germination kinetics. Comparison of the ScreenSeed procedure with commonly used germination tests based upon visual scoring displayed very similar germination kinetics.Nicolas MerieuxPierre CordierMarie-Hélène WagnerSylvie DucournauSophie AligonDominique JobPhilippe GrappinEdwin GrappinNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nicolas Merieux Pierre Cordier Marie-Hélène Wagner Sylvie Ducournau Sophie Aligon Dominique Job Philippe Grappin Edwin Grappin ScreenSeed as a novel high throughput seed germination phenotyping method
description	Abstract A high throughput phenotyping tool for seed germination, the ScreenSeed technology, was developed with the aim of screening genotype responsiveness and chemical drugs. This technology was presently used with Arabidopsis thaliana seeds to allow characterizing seed samples germination behavior by incubating seeds in 96-well microplates under defined conditions and detecting radicle protrusion through the seed coat by automated image analysis. This study shows that this technology provides a fast procedure allowing to handle thousands of seeds without compromising repeatability or accuracy of the germination measurements. Potential biases of the experimental protocol were assessed through statistical analyses of germination kinetics. Comparison of the ScreenSeed procedure with commonly used germination tests based upon visual scoring displayed very similar germination kinetics.
format	article
author	Nicolas Merieux Pierre Cordier Marie-Hélène Wagner Sylvie Ducournau Sophie Aligon Dominique Job Philippe Grappin Edwin Grappin
author_facet	Nicolas Merieux Pierre Cordier Marie-Hélène Wagner Sylvie Ducournau Sophie Aligon Dominique Job Philippe Grappin Edwin Grappin
author_sort	Nicolas Merieux
title	ScreenSeed as a novel high throughput seed germination phenotyping method
title_short	ScreenSeed as a novel high throughput seed germination phenotyping method
title_full	ScreenSeed as a novel high throughput seed germination phenotyping method
title_fullStr	ScreenSeed as a novel high throughput seed germination phenotyping method
title_full_unstemmed	ScreenSeed as a novel high throughput seed germination phenotyping method
title_sort	screenseed as a novel high throughput seed germination phenotyping method
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/a7959c52d65746699a5df85f6a0bf621
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ScreenSeed as a novel high throughput seed germination phenotyping method

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