Transcriptome analysis unveils survival strategies of Streptococcus parauberis against fish serum.

Streptococcus parauberis is an important bacterial fish pathogen that causes streptococcosis in a variety of fish species including the olive flounder. Despite its importance in the aquaculture industry, little is known about the survival strategy of S. parauberis in the host. Therefore, the objecti...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yoonhang Lee, Nameun Kim, HyeongJin Roh, Ahran Kim, Hyun-Ja Han, Miyoung Cho, Do-Hyung Kim
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a79cca96164b4c93ad3f0e9f8fef2596
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a79cca96164b4c93ad3f0e9f8fef2596
record_format	dspace
spelling	oai:doaj.org-article:a79cca96164b4c93ad3f0e9f8fef25962021-12-02T20:05:28ZTranscriptome analysis unveils survival strategies of Streptococcus parauberis against fish serum.1932-620310.1371/journal.pone.0252200https://doaj.org/article/a79cca96164b4c93ad3f0e9f8fef25962021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0252200https://doaj.org/toc/1932-6203Streptococcus parauberis is an important bacterial fish pathogen that causes streptococcosis in a variety of fish species including the olive flounder. Despite its importance in the aquaculture industry, little is known about the survival strategy of S. parauberis in the host. Therefore, the objective of this study was to produce genome-wide transcriptome data and identify key factors for the survival of S. parauberis SPOF3K in its host. To this end, S. parauberis SPOF3K was incubated in olive flounder serum and nutrient-enriched media as a control. Although S. parauberis SPOF3K proliferated in both culture conditions, the transcriptomic patterns of the two groups were very different. Interestingly, the expression levels of genes responsible for the replication of an S. parauberis plasmid in the presence of olive flounder serum were higher than those in the absence of olive flounder serum, indicating that this plasmid may play an important role in the survival and proliferation of S. parauberis in the host. Several ATP-binding cassette transporters known to transport organic substrates (e.g., biotin and osmoprotectants) that are vital for bacterial survival in the host were significantly up-regulated in S. parauberis cultured in serum. In addition, groEL, dnaK operon, and members of the clp protease family, which are known to play important roles in response to various stressors, were up-regulated in S. parauberis incubated in serum, thus limiting damage and facilitating cellular recovery. Moreover, important virulence factors including the hyaluronic acid capsule (has operon), sortase A (srtA), C5a peptidase (scp), and peptidoglycan O-acetyltransferase (oatA) were significantly upregulated in S. paraubers in serum. These results indicate that S. paraubers can resist and evade the humoral immune responses of fish. The transcriptomic data obtained in this study provide a better understanding of the mode of action of S. parauberis in fish.Yoonhang LeeNameun KimHyeongJin RohAhran KimHyun-Ja HanMiyoung ChoDo-Hyung KimPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 5, p e0252200 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yoonhang Lee Nameun Kim HyeongJin Roh Ahran Kim Hyun-Ja Han Miyoung Cho Do-Hyung Kim Transcriptome analysis unveils survival strategies of Streptococcus parauberis against fish serum.
description	Streptococcus parauberis is an important bacterial fish pathogen that causes streptococcosis in a variety of fish species including the olive flounder. Despite its importance in the aquaculture industry, little is known about the survival strategy of S. parauberis in the host. Therefore, the objective of this study was to produce genome-wide transcriptome data and identify key factors for the survival of S. parauberis SPOF3K in its host. To this end, S. parauberis SPOF3K was incubated in olive flounder serum and nutrient-enriched media as a control. Although S. parauberis SPOF3K proliferated in both culture conditions, the transcriptomic patterns of the two groups were very different. Interestingly, the expression levels of genes responsible for the replication of an S. parauberis plasmid in the presence of olive flounder serum were higher than those in the absence of olive flounder serum, indicating that this plasmid may play an important role in the survival and proliferation of S. parauberis in the host. Several ATP-binding cassette transporters known to transport organic substrates (e.g., biotin and osmoprotectants) that are vital for bacterial survival in the host were significantly up-regulated in S. parauberis cultured in serum. In addition, groEL, dnaK operon, and members of the clp protease family, which are known to play important roles in response to various stressors, were up-regulated in S. parauberis incubated in serum, thus limiting damage and facilitating cellular recovery. Moreover, important virulence factors including the hyaluronic acid capsule (has operon), sortase A (srtA), C5a peptidase (scp), and peptidoglycan O-acetyltransferase (oatA) were significantly upregulated in S. paraubers in serum. These results indicate that S. paraubers can resist and evade the humoral immune responses of fish. The transcriptomic data obtained in this study provide a better understanding of the mode of action of S. parauberis in fish.
format	article
author	Yoonhang Lee Nameun Kim HyeongJin Roh Ahran Kim Hyun-Ja Han Miyoung Cho Do-Hyung Kim
author_facet	Yoonhang Lee Nameun Kim HyeongJin Roh Ahran Kim Hyun-Ja Han Miyoung Cho Do-Hyung Kim
author_sort	Yoonhang Lee
title	Transcriptome analysis unveils survival strategies of Streptococcus parauberis against fish serum.
title_short	Transcriptome analysis unveils survival strategies of Streptococcus parauberis against fish serum.
title_full	Transcriptome analysis unveils survival strategies of Streptococcus parauberis against fish serum.
title_fullStr	Transcriptome analysis unveils survival strategies of Streptococcus parauberis against fish serum.
title_full_unstemmed	Transcriptome analysis unveils survival strategies of Streptococcus parauberis against fish serum.
title_sort	transcriptome analysis unveils survival strategies of streptococcus parauberis against fish serum.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/a79cca96164b4c93ad3f0e9f8fef2596
work_keys_str_mv	AT yoonhanglee transcriptomeanalysisunveilssurvivalstrategiesofstreptococcusparauberisagainstfishserum AT nameunkim transcriptomeanalysisunveilssurvivalstrategiesofstreptococcusparauberisagainstfishserum AT hyeongjinroh transcriptomeanalysisunveilssurvivalstrategiesofstreptococcusparauberisagainstfishserum AT ahrankim transcriptomeanalysisunveilssurvivalstrategiesofstreptococcusparauberisagainstfishserum AT hyunjahan transcriptomeanalysisunveilssurvivalstrategiesofstreptococcusparauberisagainstfishserum AT miyoungcho transcriptomeanalysisunveilssurvivalstrategiesofstreptococcusparauberisagainstfishserum AT dohyungkim transcriptomeanalysisunveilssurvivalstrategiesofstreptococcusparauberisagainstfishserum
_version_	1718375501205078016

Transcriptome analysis unveils survival strategies of Streptococcus parauberis against fish serum.

Ejemplares similares